LOCUS       sequence-32329-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC5-5XUAS-IVS-mCD8::GFP
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..671)
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     intron          692..758
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(805..826)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gatccgcggc tgtggtagca gatgagagtg atgatcaagg acagcagaag ggccacgcag
       61 attccggcca agggtgccca gatgtaaata tcacaggcga agtccaatcc ggtccccttc
      121 actgagccac ggggccgaca atcttctggt ctctggggct gagatgtccc ggtagggtgc
      181 acaggtgagg gagttcgcag cactggcttg gtagtagtag agttcacttt ctgaaggact
      241 ggcacgacag aactgaagta catcaccgag ttgctgatga ctgagcagaa atagtagcct
      301 tcgttttcct tgctgaactt gttcagggtg agaacgtact tattattcgt gtccctcatg
      361 gcagaaaaca gtttcgacga attcagcttc tcgtcccacg ttatcttgtt gtgggatgaa
      421 gccatataga caacgaaggt gggctggggg agtttggagc tggagttctg gaagagccaa
      481 gagcatcctt gcgaaacgga ccccaacact tcacatacca ggtccacctt ctgaccaagt
      541 tcggcgtcca ttttctttgg aaagattcgg agttcgggtg cctgtggctt agcttctcca
      601 ctccccagga taatcgactc acccagcagc agcaggttca gcgacagaaa gcgggtcaac
      661 ggtgaggcca ttttgctcga gccgcggccg cctgaagtaa aggataagaa ttagggttag
      721 tcgtttcggt gtgcctaggc atcagtggtt gaacctacct tttagatcta ttcagagttc
      781 tcttcttgta ttcaataatt acttcttggc agatttcagt agttgcagtt gatttacttg
      841 gttgctggtt acttttaatt gattcacttt aactt
//
