LOCUS       sequence-323297-        9705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Background-free mRNA labeling tripartite superfolder GFP system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Park SY, Moon HC, Park HY
  TITLE     Live-cell imaging of single mRNA dynamics using split superfolder 
            green fluorescent proteins with minimal background.
  JOURNAL   RNA. 2020 Jan;26(1):101-109. doi: 10.1261/rna.067835.118. Epub 2019 
            Oct 22.
  PUBMED    31641028
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.067835.118"; journalName: "RNA"; date: "2020-01"; 
            volume: "26"; issue: "1"; pages: "101-109"
COMMENT     Sequence Label: GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
FEATURES             Location/Qualifiers
     source          1..9705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1246..1255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1258..1278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1282..1308
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1282..1308
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1309..1320
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1918..1974
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1984..2004
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2008..2034
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2008..2034
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2035..2046
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2503..2559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2569..2589
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2593..2619
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2593..2619
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2620..2631
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3130..3718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3183..3203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3601..3612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3805..4038
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4110..4231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4147..4166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4201..4220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4271..4406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4426..4445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4427..4445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4455..4472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4455..4471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4464..4486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4613..5068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4700..4719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4910..4931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5094..5198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5199..5267,5268..6059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5417..5436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6230..6818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6719..6738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6972..6989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7106..7127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7142..7147,7148..7165,7166..7172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7180..7196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7185..7207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7204..7220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7204..7220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7239..7259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7241..7259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7360..7739
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7740..7943
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7894..7914
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7958..8138
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8185..8310
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8803..9036
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9221..9265
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9532..9649
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgagatccac cggtcgccac catgggccca
     1261 aaaaagaaaa gaaaagttgg ctacccctac gacgtgcccg actacgccat cgaaggccgc
     1321 catatgcgca aaggagaaga actgtttacc ggtgttgtgc caattttgat cgaactcgat
     1381 ggtgatgtca acggacataa gttcttcgtg agaggcgaag gagaaggtga cgccaccatt
     1441 ggaaaattgt cccttaaatt catctgtact actggtaaac ttcctgtacc atggccgact
     1501 ctcgtaacaa cgcttacgta cggagttcag tgcttttcga gatacccaga ccatatgaaa
     1561 agacatgact tttttaagtc ggctatgcct gaaggttacg tgcaagaaag aacaatttat
     1621 ttcaaagatg atggaaccta taaaactaga gcagaggtta aatttgaagg agatactttg
     1681 gttaaccgca ttgaactgaa aggaattgat tttaaagaag atggtaatat tcttggacac
     1741 aaactcgaat acaattttaa tagtcataag gtatacatca ctgctgataa gcaaaacaac
     1801 ggaattaaag cgaatttcac aatccgccat aatgtagaag atggcagtgt tcaacttgcc
     1861 gaccattacc aacaaaacac ccctattgga gacggtccgg tacttcttcc tggatccgct
     1921 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctggaatg
     1981 ggcccaaaaa agaaaagaaa agttggctac ccctacgacg tgcccgacta cgccatcgaa
     2041 ggccgccata tgctagccgt taaaatggct tctaacttta ctcagttcgt tctcgtcgac
     2101 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg gatcgctgaa
     2161 tggatcagct ctaactcgcg ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc
     2221 tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc ctaaaggcgc ctggcgttcg
     2281 tacttaaata tggaactaac cattccaatt ttcgccacga attccgactg cgagcttatt
     2341 gttaaggcaa tgcaaggtct cctaaaagat ggaaacccga ttccctcagc aatcgcagca
     2401 aactccggca tctacgcgga ttctagagga tccggttcta tggatcttcc tgatgaccat
     2461 tatctgagca cacagactat tttgtctaaa gatttgagcg gagctactaa cttcagcctg
     2521 ctgaagcagg ctggagacgt ggaggagaac cctggacctg caatgggccc aaaaaagaaa
     2581 agaaaagttg gctaccccta cgacgtgccc gactacgcca tcgaaggccg ccatctagcc
     2641 tccaaaacca tcgttctttc ggtcggcgag gctactcgca ctctgactga gatccagtcc
     2701 accgcagacc gtcagatctt cgaagagaag gtcgggcctc tggtgggtcg gctgcgcctc
     2761 acggcttcgc tccgtcaaaa cggagccaag accgcgtatc gcgtcaacct aaaactggat
     2821 caggcggacg tcgttgattc cggacttccg aaagtgcgct acactcaggt atggtcgcac
     2881 gacgtgacaa tcgttgcgaa tagcaccgag gcctcgcgca aatcgttgta cgatttgacc
     2941 aagtccctcg tcgcgacctc gcaggtcgaa gatcttgtcg tcaaccttgt gccgctgggc
     3001 cgtgcggggg gcgggagcgg tggctctggt tcttctgcta gtggcggctc aacatctgag
     3061 aagagagacc acatggtgct gctggagtac gtgaccgctg ccggtattac tgacgcttca
     3121 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3181 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3241 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3301 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3361 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3421 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3481 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     3541 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3601 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3661 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     3721 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3781 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3841 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3901 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3961 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4021 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4081 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4141 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4321 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4381 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4441 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4501 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4561 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5041 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7021 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7081 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7141 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7201 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7261 aacaaaagct ggagctgcaa gcttggccat tgcatacgtt gtatccatat cataatatgt
     7321 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
     7381 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     7441 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7501 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     7561 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     7621 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7681 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7741 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7801 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     7861 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     7921 gggaggtcta tataagcaga gctcgtttag tgaaccgggg tctctctggt tagaccagat
     7981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8161 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8221 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8281 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8341 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8401 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8461 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8521 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8581 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8641 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8701 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8761 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8821 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8881 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8941 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9001 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9061 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9121 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9181 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9241 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9301 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9361 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9421 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     9481 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     9541 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9601 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
     9661 gactagcctc gagaagcttg attaacccgt gtcggctcca gatct
//
