LOCUS       sequence-32332-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC7-20XUAS-IVS-mCD8::GFP
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..149
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    183..277
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    311..405
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    439..533
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 gtagacgaag cgcctctatt tatactccgg cgctcgacgt cgctagagtc tccgctcgga
       61 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga
      121 cagtactccg ctcggaggac agtactccga cctgcaggca tgcactagtg ctagagtctc
      181 cgctcggagg acagtactcc gctcggagga cagtactccg ctcggaggac agtactccgc
      241 tcggaggaca gtactccgct cggaggacag tactccgacc tgcaggcatg cgctagcgct
      301 agagtctccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
      361 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgacctg caggcatgcc
      421 ctagggctag agtctccgct cggaggacag tactccgctc ggaggacagt actccgctcg
      481 gaggacagta ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgacctgca
      541 ggcatgcaag cttgcggccc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
      601 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
      661 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
      721 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
      781 tccgga
//
