LOCUS       sequence-32333-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC7-20XUAS-IVS-mCD8::GFP
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..667)
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     intron          688..754
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 cgcggctgtg gtagcagatg agagtgatga tcaaggacag cagaagggcc acgcagattc
       61 cggccaaggg tgcccagatg taaatatcac aggcgaagtc caatccggtc cccttcactg
      121 agccacgggg ccgacaatct tctggtctct ggggctgaga tgtcccggta gggtgcacag
      181 gtgagggagt tcgcagcact ggcttggtag tagtagagtt cactttctga aggactggca
      241 cgacagaact gaagtacatc accgagttgc tgatgactga gcagaaatag tagccttcgt
      301 tttccttgct gaacttgttc agggtgagaa cgtacttatt attcgtgtcc ctcatggcag
      361 aaaacagttt cgacgaattc agcttctcgt cccacgttat cttgttgtgg gatgaagcca
      421 tatagacaac gaaggtgggc tgggggagtt tggagctgga gttctggaag agccaagagc
      481 atccttgcga aacggacccc aacacttcac ataccaggtc caccttctga ccaagttcgg
      541 cgtccatttt ctttggaaag attcggagtt cgggtgcctg tggcttagct tctccactcc
      601 ccaggataat cgactcaccc agcagcagca ggttcagcga cagaaagcgg gtcaacggtg
      661 aggccatttt gctcgagccg cggccgcctg aagtaaagga taagaattag ggttagtcgt
      721 ttcggtgtgc ctaggcatca gtggttgaac ctacctttta gatctattca gagttctctt
      781 cttgtattca ataattactt cttggcagat ttcagtag
//
