LOCUS       sequence-32335-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC8-40XUAS-IVS-mCD8::GFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..157
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    191..285
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    319..413
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    447..541
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    577..671
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    705..799
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 gtcgctccgt agacgaagcg cctctattta tactccggcg ctcgacgtcg ctagagtctc
       61 cgctcggagg acagtactcc gctcggagga cagtactccg ctcggaggac agtactccgc
      121 tcggaggaca gtactccgct cggaggacag tactccgacc tgcaggcatg cactagtgct
      181 agagtctccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
      241 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgacctg caggcatgcg
      301 ctagcgctag agtctccgct cggaggacag tactccgctc ggaggacagt actccgctcg
      361 gaggacagta ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgacctgca
      421 ggcatgccct agggctagag tctccgctcg gaggacagta ctccgctcgg aggacagtac
      481 tccgctcgga ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc
      541 gacctgcagg catgcaagct gtcgctagag tctccgctcg gaggacagta ctccgctcgg
      601 aggacagtac tccgctcgga ggacagtact ccgctcggag gacagtactc cgctcggagg
      661 acagtactcc gacctgcagg catgcactag tgctagagtc tccgctcgga ggacagtact
      721 ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga cagtactccg
      781 ctcggaggac agtactccga cctgcaggca tgcgctagcg ctagagtctc cgctcgga
//
