LOCUS       sequence-32336-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC8-40XUAS-IVS-mCD8::GFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..670)
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     intron          691..757
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(804..825)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 atccgcggct gtggtagcag atgagagtga tgatcaagga cagcagaagg gccacgcaga
       61 ttccggccaa gggtgcccag atgtaaatat cacaggcgaa gtccaatccg gtccccttca
      121 ctgagccacg gggccgacaa tcttctggtc tctggggctg agatgtcccg gtagggtgca
      181 caggtgaggg agttcgcagc actggcttgg tagtagtaga gttcactttc tgaaggactg
      241 gcacgacaga actgaagtac atcaccgagt tgctgatgac tgagcagaaa tagtagcctt
      301 cgttttcctt gctgaacttg ttcagggtga gaacgtactt attattcgtg tccctcatgg
      361 cagaaaacag tttcgacgaa ttcagcttct cgtcccacgt tatcttgttg tgggatgaag
      421 ccatatagac aacgaaggtg ggctggggga gtttggagct ggagttctgg aagagccaag
      481 agcatccttg cgaaacggac cccaacactt cacataccag gtccaccttc tgaccaagtt
      541 cggcgtccat tttctttgga aagattcgga gttcgggtgc ctgtggctta gcttctccac
      601 tccccaggat aatcgactca cccagcagca gcaggttcag cgacagaaag cgggtcaacg
      661 gtgaggccat tttgctcgag ccgcggccgc ctgaagtaaa ggataagaat tagggttagt
      721 cgtttcggtg tgcctaggca tcagtggttg aacctacctt ttagatctat tcagagttct
      781 cttcttgtat tcaataatta cttcttggca gatttcagta gttgcagttg atttacttgg
      841 ttgctggtta cttttaattg attcacttta acttgca
//
