LOCUS       sequence-323391-        9261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic transgenesis 5xGMR white CDS physical eye marker cassette in 
            Alpha2, lacks attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pGMR-WhiteA2
FEATURES             Location/Qualifiers
     source          1..9261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    145..618
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(691..1662)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1662..2837)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3163..3413
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3416..4171)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4262..4481
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4568..5203
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5317..5419
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5420..6235
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5420..6235
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5493..5512)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     polyA_signal    8830..8964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8854..8873)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8908..8927
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    9177..9246
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cttctaagca ggacacagca gcaatccaca gcaggcatac aaccgcacac cgaggttact
       61 ccgttctaca ggttacgacg acatgtcaat acttgccctt gacaggcatt gatggaatcg
      121 tagtctcacg ctgatagtct gatcgacaat acaagtggga ccgtggtccc agaccgataa
      181 tcagaccgac aacacgagtg ggatcgtggt cccagactaa taatcagacc gacgatacga
      241 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggttcca
      301 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg
      361 acgatacgag tgggaccatg gtcccagact aataatcaga ccgacgatac gagtgggacc
      421 gtggtcccag tctgattatc agaccgacga tacgagtggg accgtggtcc cagactaata
      481 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg
      541 agtgggaccg tggtcccagt ctgattatca gaccgacgat acaagtggaa cagtgggccc
      601 agagagaata ttcaggccag ttatgctttc tggcctgtaa caaaggacat taagtaaaga
      661 cagataaacg tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa gttccttaag
      721 aatggcctca attttctcta tacactcagt tggaacacgc gacctgtcca ggttaagcac
      781 cattttatcg cccttataca atactgtcgc tccaggagca aactgatgtc gtgagcttaa
      841 actagttctt gatgcagatg acgttttaag cacagaagtt aaaagagtga taacttcttc
      901 agcttcaaat atcaccccag cttttttctg ctcatgaagg ttagatgcct gctgcttaag
      961 taattcctct ttatctgtaa aggctttttg aagtgcatca cctgaccggg cagatagttc
     1021 accggggtga gaaaaaagag caacaactga tttaggcaat ttggcggtgt tgatacagcg
     1081 ggtaataatc ttacgtgaaa tattttccgc atcagccagc gcagaaatat ttccagcaaa
     1141 ttcattctgc aatcggcttg cataacgctg accacgttca taagcacttg ttgggcgata
     1201 atcgttaccc aatctggata atgcagccat ctgctcatca tccagctcgc caaccagaac
     1261 acgataatca ctttcggtaa gtgcagcagc tttacgacgg cgactcccat cggcaatttc
     1321 tatgacacca gatactcttc gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga
     1381 agggatgaga tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt cattacctga
     1441 ccatacccga gaggtctttt caacactatc accccggagc acttcaagag taaacttcac
     1501 atcccgacca catacaggca aagtaatggc attaccgcga gccattactc ctacgcgcgc
     1561 aattaacgaa tccaccatcg gggcagctgg tgtcgataac gaagtatctt caaccggttg
     1621 agtattgagc gtatgttttg gaataacagg cgcacgcttc attatctaat ctcccagcgt
     1681 ggtttaatca gacgatcgaa aatttcattg cagacaggtt cccaaataga aagagcattt
     1741 ctccaggcac cagttgaaga gcgttgatca atggcctgtt caaaaacagt tctcatccgg
     1801 atctgacctt taccaacttc atccgtttca cgtacaacat tttttagaac catgcttccc
     1861 caggcatccc gaatttgctc ctccatccac ggggactgag agccattact attgctgtat
     1921 ttggtaagca aaatacgtac atcaggctcg aaccctttaa gatcaacgtt cttgagcaga
     1981 tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg
     2041 ggaacaatca gcacatcagc agcacatacg acattaatcg tgccgatacc caggttaggc
     2101 gcgctgtcaa taactatgac atcatagtca tgagcaacag tttcaatggc cagtcggagc
     2161 atcaggtgtg gatcggtggg cagtttacct tcatcaaatt tgcccattaa ctcagtttca
     2221 atacggtgca gagccagaca ggaaggaata atgtcaagcc ccggccagca agtgggcttt
     2281 attgcataag tgacatcgtc cttttcccca agatagaaag gcaggagagt atcttctgca
     2341 tgaatatgaa gatctggtac ccatccgtga tacattgagg ctgttccctg ggggtcgtta
     2401 ccttccacga gcaaaacacg tagccccttc agagccagat cctgagcaag atgaacagaa
     2461 actgaggttt tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg tggaaataca
     2521 tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca tatgattaat ttgttcaatt
     2581 gtataaccaa cacgttgctc aacccgtcct cgaatttcca tatccgggtg cggtagtcgc
     2641 cctgctttct cggcatctct gatagcctga gaagaaaccc caactaaatc cgctgcttca
     2701 cctattctcc agcgccgggt tattttcctc gcttccgggc tgtcatcatt aaactgtgca
     2761 atggcgatag ccttcgtcat ttcatgacca gcgtttatgc actggttaag tgtttccatg
     2821 agtttcattc tgaacatcct ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt
     2881 tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata
     2941 agagcaacac tacaaaagga gataagaaga gcacatacct cagtcactta ttatcactag
     3001 cgctcgccgc agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag caaagaagaa
     3061 ctgttctgtc agatagctct tacgctcagc gcaagaagaa atatccaccg tgggaaaaac
     3121 tccaggtaga ggtacacacg cggatagcca attcagagta ataaactgtg ataatcaacc
     3181 ctcatcaatg atgacgaact aacccccgat atcaggtcac atgacgaagg gaaagagaag
     3241 gaaatcaact gtgacaaact gccctcaaat ttggcttcct taaaaattac agttcaaaaa
     3301 gtatgagaaa atccatgcag gctgaaggaa acagcaaaac tgtgacaaat taccctcagt
     3361 aggtcagaac aaatgtgacg aaccaccctc aaatctgtga cagataaccc tcagactatc
     3421 ctgtcgtcat ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt
     3481 tctttttctc aatgtatgag aggcgcattg gagttctgct gttgatctca ttaacacaga
     3541 cctgcaggaa gcggcggcgg aagtcaggca tacgctggta actttgaggc agctggtaac
     3601 gctctatgat ccagtcgatt ttcagagaca cgatgcctga gccatccggc ttacgatact
     3661 gacacaggga ttcgtataaa cgcatggcat acggattggt gatttctttt gtttcactaa
     3721 gccgaaactg cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga tatgggttga
     3781 tatgtacact gtaaagccct ctggatggac tgtgcgcacg tttgataaac caaggaaaag
     3841 attcatagcc tttttcatcg ccggcatcct cttcagggcg ataaaaaacc acttccttcc
     3901 ccgcgaaact cttcaatgcc tgccgtatat ccttactggc ttccgcagag gtcaatccga
     3961 atatttcagc atatttagca acatggatct cgcagatacc gtcatgttcc tgtagggtgc
     4021 catcagattt tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga
     4081 gactatatgc cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt
     4141 tcttgtgatt gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt
     4201 ttagattgtc acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca
     4261 gcttcttctg agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc
     4321 atttgagggt gatttgtcac actgaaaggg caatttgtca caacaccttc tctagaacca
     4381 gcatggataa aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat
     4441 aattataaaa atatccccgt ggataagtgg ataaccccaa gggaagtttt ttcaggcatc
     4501 gtgtgtaagc agaatatata agtgctgttc cctggtgctt cctcgctcac tcgaaattcc
     4561 cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     4621 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cggccgccgg
     4681 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     4741 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     4801 ccggcgacgt ggagctggcc agcctcgcaa atctgcgaaa acgcctgatt ttacgcgagt
     4861 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     4921 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     4981 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     5041 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     5101 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     5161 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     5221 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg caggggatct
     5281 cgagggcttc gccctgtcgc tcgactgcgg cggtgatgat cggcacgtaa gaggttccaa
     5341 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     5401 aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct caaggccgcg
     5461 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     5521 gcaatcaggt gcgacaatct accgattgta tgggaagccc gatgcgccag agttgtttct
     5581 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     5641 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     5701 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
     5761 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     5821 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgactcgctc aggcgcaatc
     5881 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     5941 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtagt
     6001 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     6061 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     6121 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     6181 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctgattttt
     6241 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     6301 agcggatgaa tggcagaaat tcgatgataa gctgtcaaac atgataacaa gcttcgtctc
     6361 agtcaggagc gtacgactag cacctgactg tcgagcccag tggaaaccct tgaaatgcct
     6421 ttaagtcgag cccagtggaa acccttgaaa tgcctttaag tcgagcccag tggaaaccct
     6481 tgaaatgcct ttaagtcgag cccagtggaa acccttgaaa tgcctttaag tcgagcccag
     6541 tggaaaccct tgaaatgcct ttaagtcgag tagcagtcga ggtaggtacg tcatgcccct
     6601 cccgagctcg cccggggatc gagcgcagcg gtataaaagg gcgcggggtg gctgagagca
     6661 tcagttgtga atgaatgttc gagccgagca gacgtgccgc tgccttcgtt aatatccttt
     6721 gaataagcca actttgaatc acaagacgca taccaaacca aatgggacag gaggaccagg
     6781 agctgctgat ccgcggaggc tccaagcacc caagcgccga gcacctgaac aacggcgata
     6841 gcggagccgc cagccagtcg tgcatcaatc agggatttgg ccaggccaag aattacggaa
     6901 ccctgctgcc ccccagcccc ccagaggata gcggaagcgg atccggacag ctggccgaga
     6961 atctgacgta cgcctggcat aacatggata tcttcggcgc cgtgaaccag ccaggcagtg
     7021 gctggcgtca gctggtgaac cgcacccgcg gactgttctg caatgagcgc catatcccag
     7081 ccccacgcaa gcacctgctg aagaatgtgt gcggagtggc ctaccccggc gagctgctgg
     7141 ccgtgatggg aagtagtggc gccggaaaga ccacgctgct gaacgccctg gccttccgca
     7201 gcccccaggg cattcaggtg tccccaagcg gcatgcgcct gctgaacggc cagcccgtgg
     7261 atgccaagga gatgcaggcc cgctgcgcct acgtgcagca ggacgatctg ttcatcggca
     7321 gcctgacagc ccgcgagcat ctgatctttc aggccatggt ccgcatgccc cgccacctga
     7381 cataccgtca gcgcgtggcc cgcgtggacc aggtcatcca ggagctgagc ctgagcaagt
     7441 gccagcacac cattattggc gtgccaggcc gcgtgaaggg cctgagtggc ggagagcgca
     7501 agcgcctggc ctttgccagc gaggccctga ccgatccccc cctgctgatt tgcgacgagc
     7561 cgaccagcgg cctggatagc ttcaccgccc actccgtggt gcaggtgctg aagaagctga
     7621 gccagaaggg aaagacggtc attctgacga tccaccagcc cagctcggag ctgttcgagc
     7681 tgtttgataa gattctgctg atggccgagg gacgcgtggc cttcctggga accccaagcg
     7741 aggccgtgga tttcttcagc tatgtgggcg cccagtgccc cacgaattac aaccccgccg
     7801 acttctatgt gcaagtgctg gccgtggtgc caggacgcga gattgagagc cgcgatcgca
     7861 ttgccaagat ctgcgataac ttcgccatct ccaaggtggc ccgcgacatg gagcagctgc
     7921 tggccacgaa gaacctggag aagcccctgg agcagcccga gaacggctat acgtataagg
     7981 ctacctggtt tatgcagttt cgcgccgtgc tgtggcgcag ctggctgagt gtgctgaagg
     8041 agcccctgct ggtcaaggtc cgcctgatcc agaccacgat ggtggccatt ctgatcggcc
     8101 tgatcttcct gggacagcag ctgacgcagg tcggagtgat gaacatcaat ggagccattt
     8161 ttctgtttct gacgaacatg acgttccaga acgtgttcgc caccatcaac gtgttcacca
     8221 gcgagctgcc cgtgttcatg cgcgaggccc gcagccgcct gtaccgctgc gatacctact
     8281 tcctgggaaa gaccattgcc gagctgccgc tgttcctgac cgtgcccctg gtgtttaccg
     8341 ccattgccta ccccatgatt ggcctgcgtg ccggagtgct gcatttcttt aattgcctgg
     8401 ccctggtgac cctggtggcc aacgtgtcga ccagcttcgg ctacctgatc agctgcgcct
     8461 ccagcagcac cagcatggcc ctgagcgtgg gaccccccgt gattatcccc ttcctgctgt
     8521 tcggcggatt ctttctgaac tccggcagcg tgcccgtgta cctgaagtgg ctgagctacc
     8581 tgagctggtt tcgctacgcc aatgagggcc tgctgatcaa tcagtgggcc gatgtggagc
     8641 ccggcgagat ctcctgcacc agcagcaaca cgacctgccc cagcagcgga aaggtcatcc
     8701 tggagacact gaatttcagt gccgccgacc tgccactgga ttatgtgggc ctggccatcc
     8761 tgattgtgtc cttccgcgtg ctggcctatc tggctctgcg cctgcgcgct cgtcgcaagg
     8821 agtgagcttg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     8881 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     8941 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     9001 cagggggagg tgtgggaggt tttttcagta tggcaagcgc tcgacgaatt aacaagcttc
     9061 gctctatctc gctctctatc tctctcgatc gctcgtgcta agcttaagac cgtggagaac
     9121 aaggagaacc gcttgagacg aagcttgacg aattggtcga cgatgtaggt cactgtctcg
     9181 aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca
     9241 cccatctggt ccatcatgat g
//
