LOCUS       sequence-32340-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC14-10XUAS-IVS-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..740)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          770..836
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 taatccagag gttgatttct agattacttg tagagctcat ccatgccgtg tgtaatacca
       61 gctgcggtca caaactccag caacaccatg tggtcccgct tttcattggg gtccttgctc
      121 aacgcggact gtgtggaaag gtagtgattg tctggcaaga gaactgggcc atctccaatg
      181 ggtgtgttct gttggtaatg atctgcaagt tgcacggaac cgtcctctat gttgtggcga
      241 atcttgaagt taacctttat tccgtttttc tgcttatctg ccattatata cacattatgc
      301 gagttgtagt tgtattccaa cttatggccc agaatgttgc catcctcctt aaaatcaatg
      361 cccttcaact caatacggtt aacgagtgtg tcgccttcaa atttcacctc cgctcgcgtc
      421 ttatagttgc cgtcatcctt gaagaaaatg gtcctctctt gaacgtatcc ctccggcatc
      481 gccgacttga aaaagtcatg ctgcttcata tgatcggggt aacggctgaa acactggaca
      541 ccatacgtca gcgtcgtgac caacgtcggc catggcacgg gcagcttgcc cgtggtgcat
      601 atgaacttca acgtcagctt accgtatgtg gcgtcgccct caccctcgcc ggacacactg
      661 aatttatgac cgttgacatc gccgtcgagt tcaaccaata tcgggactac tccggtaaac
      721 agttcttcac ctttggacat tttgattggt accctcgagc cgcggccgcc tgaagtaaag
      781 gataagaatt agggttagtc gtttcggtgt gcctaggcat cagtggttga acctaccttt
      841 tagatctatt cagagttctc ttct
//
