LOCUS       sequence-323444-        9315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector.
            Contains an Alpha level GB2.0 entry point for addition of custom 
            assemblies utilizing blue-white bacterial colony screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-5xQUAS-mCherry-MiniWhite
FEATURES             Location/Qualifiers
     source          1..9315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(511..1302)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1770..2358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2259..2278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3125..3739,3810..3941,4145..4460,4522..5176,
                     5251..5524,5926..5997))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6449..6467
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     polyA_signal    6555..6689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6592..6611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6646..6665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(8233..8684)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(8264..8285)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    8712..8781
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     8838..8857
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9051..9073
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9065..9082
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9066..9082
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9089..9108
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9089..9107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    9110..9166
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(9173..9191)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9174..9191)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(9245..9292)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgctggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaca tgaagtttta aatcaatcta
      481 aagtatatat gtgtaacatt ggtctagtga ttatttgccg actaccttgg tgatctcgcc
      541 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaaac gatcttcttc
      601 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
      661 ctccattgcc cagtcggcag ctacatcctt cggcgcgatt ttgccggtta ctgcgctgta
      721 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
      781 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
      841 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
      901 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
      961 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     1021 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     1081 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     1141 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     1201 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     1261 tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta actttgtttt
     1321 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     1381 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
     1441 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     1501 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
     1561 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
     1621 tagcagcgaa gtcgaggcat ttctgtcctg gctggtcaca aaaggatcta ggtgaagatc
     1681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgtc
     2461 aagcgatgca tctacacaag gaacaaacac tggatgtcac tttcagttca aattgtaacg
     2521 ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat taaattagaa
     2581 taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat tgtacataca
     2641 aatatttatt actaatttct attgagccga aatgaaccac tcggaaccat ttgagcgaac
     2701 cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt
     2761 gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga
     2821 attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt
     2881 atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga
     2941 agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg
     3001 tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa
     3061 cagtaaacac gatggtaata atggtaaaaa aaaaaaacaa gcagttattt cggatatatg
     3121 tcggctactc cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc acccggaagc
     3181 tcacgatgag aatggccaga cccacgtagt ccagcggcag atcggcggcg gagaagttaa
     3241 gtgtctccag gatgaccttg cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa
     3301 tttcgcccgg ctccacgtcc gcccattggt taatcagcag accctcgttg gcgtaacgga
     3361 accatgagag gtacgacaac catttgaggt atactggcac cgagcccgag ttcaagaaga
     3421 agccgccaaa gagcaggaat ggtatgataa ccggcggacc cacagacagc gccatcgagg
     3481 tcgaggagct ggcgcaggat attagatatc cgaaggacgt tgacacattg gccaccagag
     3541 tgaccagcgc caggcagttg aagaagtgca gcactccggc ccgcagtccg atcatcggat
     3601 aggcaatcgc cgtgaagacc agtggcactg tgagaaaaag cggcaattcg gcaatcgttt
     3661 tgcccagaaa gtatgtgtca cagcgataaa gtcgacttcg ggcctccctc ataaaaactg
     3721 gcagctctga ggtgaacacc taaatcgaat cgattcatta gaaagttagt aaattattga
     3781 aatgcaaatg tattctaaac atgacttaca tttatcgtgg caaagacgtt ttgaaaggtc
     3841 atgttggtca ggaagaggaa gatggctccg ttgatattca tcacacccac ttgcgtgagt
     3901 tgttggccca aaaagatgag gccaatcaag atggcaacca tctgcaaatt aaaatgttac
     3961 tcgcatctca ttaatattcg cgagttaaat gaaatttatt tatcttctgc aaaactataa
     4021 actatacatc tcattgaaaa aaactaagaa gggtgtggaa tcaggcaatt ctatctaaaa
     4081 tctagcgaat ttgtttccaa gaattgtaag cgttatatca tttgtttcca ctggaaccac
     4141 tcaccgttgt ctgaataagt cgcactttta cgaggagtgg ttccttgagc accgacagcc
     4201 aggatcgcca caggaccgcc cggaactgca tgaaccaggt ggccttgtag gtgtacccat
     4261 tctccggctg ctccagtggc ttctccagat ttttggtggc caacaactgc tccatatccc
     4321 gggctacttt gctaatggca aaattgtcgc atatcttggc gatccgatca cgggactcga
     4381 tctcccgtcc gggcacaacg gccaacacct gtacgtaaaa gtccgccgga ttgtagttgg
     4441 taggacactg ggcacccacg ctggatagga gttgagatgt tatgtaatac tagataccct
     4501 taataaacac atcgaactca ctaggaaaag aagtcgacgg cttcgctggg agtgcccaag
     4561 aaagctaccc tgccctcggc catcagaagg atcttgtcaa agagctcaaa cagctcggaa
     4621 gacggctgat gaatggtcag gatgacggtc ttgcccttct gcgacagctt cttcagcacc
     4681 tggacgacgc tgtgggcggt aaaggagtcc agtccggagg tgggctcatc gcagatcaga
     4741 agcggcggat cggttagagc ctcggaggcg aatgccagac gcttcctttc tccgccggac
     4801 agacctttca ccctgccggg cacaccgatg atcgtgtgct gacatttgct gagcgaaagc
     4861 tcctggatca cctgatccac gcgggccact cgctgccgat aggtcagatg tcgtggcatc
     4921 cgcaccatgg cttggaaaat caggtgttcc ctggccgtta gggagccgat aaagaggtca
     4981 tcctgctgga cataggcgca cctggcctgc atctccttgg cgtccacagg ttggccattg
     5041 agcagtcgca tcccggatgg cgatacttgg atgccctgcg gcgatcgaaa ggcaagggca
     5101 ttcagcaggg tcgtctttcc ggcaccggaa ctgcccatca cggccaaaag ttcgcccgga
     5161 taggccacgc cgcaaactga gtttcaaatt ggtaattgga ccctttatta agatttcaca
     5221 cagatcagcc gactgcgaat agaaactcac cgttcttgag caaatgtttc ctgggcgccg
     5281 gtatgtgtcg ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc cgccatccgg
     5341 agcccggctg attgaccgcc ccaaagatgt ccatattgtg ccaggcatag gtgaggttct
     5401 cggctagttg gccgctccct gaaccggagt cctccggcgg actgggtggc cggagcgtgc
     5461 cgtagttttt ggcctgcccg aagccctggt taatgcagct ctgcgaagcc gctccgctgt
     5521 caccctgcaa tgatagggga tctcaaatat caactacaag cgttatgctc atctaacccc
     5581 gaacaaaaag taccccgaag tatcctacga agtaggttta tacttttatt tattttttgt
     5641 gcatctagga tcagcttaaa atatctggtt gttatatttt ttgtaaaaaa gaatatagtc
     5701 gaaaatgaat gcctttagat gtcttgatca tgatatgatc tcaaaaattg tcttatatag
     5761 cgagaacagc taccagaata atctgtttcg tgtcactatt tgtttgtgca attgcggttt
     5821 gggatttttg tgggtcgcag ttctcacgcc gcagacaatt tgatgttgca atcgcagttc
     5881 ctatagatca agtgaactta agatgtatgc acatgtacta ctcacattgt tcagatgctc
     5941 ggcagatggg tgtttgctgc ctccgcgaat taatagctcc tgatcctctt ggcccattgc
     6001 cgggattttt cacactttcc cctgcttacc cacccaaaac caatcaccac cccaatcact
     6061 caaaaaacaa acaaaaataa gaagcgagag gagttttggc acagcacttt gtgtttaatt
     6121 gatggcgtaa accgcttgga gcttcgtcac gaaaccgctg acaaaatgca actgaaggcg
     6181 gacattgacg ctacgtaacg ctacaaacgg tggcgaaaga gatagcggac gcagcggcga
     6241 aagtgacggc gatatttctg tggacagaga aggaggcaaa cagcgctgac tttgagtgga
     6301 atgtcatttt gagtgagagg taatcgaaag aacctggtac atcaaatacc cttggatcga
     6361 agtaaattta aaactgatca gataagttca atgatatcca gtgcagtaaa aaaaaaaaat
     6421 gtttttttta tctactttcc gcaaaaatgg gttttattaa cttacataca tactagaatt
     6481 cctcctgaca gcgaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6541 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6661 tcatcaatgt atcttatcat gtctggatca agcttacttg tacagctcat ccatgccgcc
     6721 cgtgctatga cgtccttcgg cacgctcgta ctgctccacg atggtgtagt cctcgttgtg
     6781 gctggtgatg tccagcttga tgttcacgtt gtaggcgccg ggcaattgca cgggcttctt
     6841 ggccttgtag gtggtcttca cctcggcgtc gtagtggcct ccatccttca gcttcaggcg
     6901 ctgcttgatc tcgcccttca gagcgccgtc ctcgggatac atgcgctcgc tggaagcctc
     6961 ccagcccatg gtcttcttct gcatcaccgg gccatcggag ggaaagttcg tgccgcgcag
     7021 cttgaccttg tagatgaact cgccgtcctg caagctggag tcctgggtca cggtcaccac
     7081 tccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccttcgggga agctcagctt
     7141 caggtagtcg ggaatgtcgg ccgggtgctt cacgtaggcc ttggagccgt acatgaactg
     7201 ggggctcaag atgtcccagg caaagggcaa cgggcctccc ttggtcacct tcagtttggc
     7261 ggtctgggtg ccttcgtagg gacgtccctc gccttcgccc tcgatctcga actcgtggcc
     7321 attcacgctg ccctccatgt ggaccttgaa gcgcatgaac tccttgatga tggccatgtt
     7381 atcctcttct cccttggaca ccatttggtt tggtatgcgt cttgtgattc aaagttggct
     7441 tattcaaagg atattaacga aggcagcggc acgtctgctc ggctcgaaca ttcattcaca
     7501 actgatgctc tcagccaccc cgcgcccttt tataccgctg cgctcgatcc ccgggcgagc
     7561 tcgggaaagg ataagcgatt acccgaggat aagcgattac ccggataagc gattacccgt
     7621 gaggataatt gtttatccga ggataagcga ttacccctcc tgacagcgga acaaacgacc
     7681 caacacccgt gcgttttatt ctgtcttttt attgccaagc ttagttacgg gtatacttca
     7741 ggggaatcag ttcctcaatg gtggtcttga ccagcttgcc gttcatctca atcagcacga
     7801 agcaatcggg ggcgtaatcg ctaatcagct cacggcacat tccacacggg gacacgacac
     7861 ggatgctacg gtccacctca tcggagtatg ggtgacgcac ggccacaatg gtatcaaaat
     7921 ccttctggcc gttgctcacg gcactgccaa tggcaatggc ctcggcacac acggtcacgc
     7981 gtccgatgta ggcctcaatg tgcacggcac tgataatctc gcccgtcttg gtgcgaatgg
     8041 cagctcccac gtggtgcttg ttatcctcgt agagcatcgt aatcttctcg gtagccacct
     8101 ccaccagttc caggtcctgc tggctgatgt tgaaggtttt cattttgttt tcctccttat
     8161 gttaagctta ctcagttatt atatcataaa tatctgtgtc aagaataaac tcccacatgg
     8221 attcgagtat tgagagttct cttcttgtat tcaataatta cttcttggca gatttcagta
     8281 gttgcagttg atttacttgg ttgctggtta cttttaattg attcacttta acttgcactt
     8341 tactgcagat tgtttagctt gttcagctgc gcttgtttat ttgcttagct ttcgcttagc
     8401 gacgtgttca ctttgcttgt ttgaattgaa ttgtcgctcc gtagacgaag cgcctctatt
     8461 tatactccgg cgctcttttc gcgaacattc gaggcgcgct ctctcgaacc aacgagagca
     8521 gtatgccgtt tactgtgtga cagagtgaga gagcattagt gcagagaggg agcgacccaa
     8581 aaagaaaaga gagaataacg aataacggcc agagaaattt ctcgagtttt ctttctgcca
     8641 aacaaatgac ctaccacaat aaccagtttg ttttgggatt ctagctcctg acagcgcatc
     8701 atgatggacc agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg
     8761 cacccgcacc gcggcttcga gacagtgacc tacatcgtcg accaattcct ccctgggatc
     8821 cggagagaga ccctatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     8881 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgtcatt cgccattcag
     8941 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     9001 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9061 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattcgagct
     9121 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttgagt attctatagt
     9181 gtcacctaaa tagcttggcg taatcatggt catgatctgc ctcctgggtt tagttcctca
     9241 ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca acaggggtct
     9301 ctcgctgatc cgtta
//
