LOCUS       sequence-323465-        7134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For light-inducible Cre recombinase (LiCre) expression under the 
            Met17 promoter in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Duplus-Bottin H, Spichty M, Triqueneaux G, Place C, Mangeot PE, 
            Ohlmann T, Vittoz F, Yvert G
  TITLE     A single-chain and fast-responding light-inducible Cre recombinase 
            as a novel optogenetic switch.
  JOURNAL   Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268.
  PUBMED    33620312
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-23"; volume: "10. pii: 61268. doi"; pages: " 
            10.7554/eLife.61268"
COMMENT     Sequence Label: pGY466
FEATURES             Location/Qualifiers
     source          1..7134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2826..3175
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     3176..3192
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3176..3192
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3226..3242)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3227..3243)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             3257..3274
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4984..5231
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5000..5018)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5249..5268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5250..5268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5278..5295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5278..5294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5287..5309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5439..5894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5526..5545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5736..5757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5993..6667)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6668..6948)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(6965..6984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7084..7106
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctccggatgc aagggttcga atcccttagc
     2821 tctcattatt ttttgctttt tctcttgagg tcacatgatc gcaaaatggc aaatggcacg
     2881 tgaagctgtc gatattgggg aactgtggtg gttggcaaat gactaattaa gttagtcaag
     2941 gcgccatcct catgaaaact gtgtaacata ataaccgaag tgtcgaaaag gtggcacctt
     3001 gtccaattga acacgctcga tgaaaaaaat aagatatata taaggttaag taaagcgtct
     3061 gttagaaagg aagtttttcc tttttcttgc tctcttgtct tttcatctac tatttccttc
     3121 gtgtaataca gggtcgtcag atacatagat acaattctat tacccccatc catactctag
     3181 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     3241 cgacatgaga ggttcacatc atcaccacca tcacggttct ttagcaacaa ctctggaaag
     3301 aatcgagaaa aacttcgtca tcacggaccc gaggctaccc gacaacccta tcattttcgc
     3361 atccgactct tttttacaat taactgaata ttctagagaa gaaattctgg gccgtaattg
     3421 taggttttta cagggtccag aaacagatag agcaacagtt agaaagatta gagatgctat
     3481 tgataatcag actgaggtaa cagttcaact aataaactat actaaatccg ggaagaaatt
     3541 ttggaatgtc ttccacttac agccaatgag agattacaaa ggtgatgtcc agtattttat
     3601 cggagttcag ttagacggca cagaacgtct gcacggtgcc gcggagaggg aagctgtttg
     3661 tcttattaag aaaaccgcat ttcaaatcga cagtgatgag gttcgcaaga acctgatgga
     3721 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
     3781 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga
     3841 agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca
     3901 gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga
     3961 cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga
     4021 acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat
     4081 ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa
     4141 caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga
     4201 cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt
     4261 agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg
     4321 tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc
     4381 gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca
     4441 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
     4501 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
     4561 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
     4621 tagtgaaaca ggggcaatgg tgcgcctgct ggcagctggc gattagccat taacgcgtaa
     4681 atgattgcta taattatttg atatttatgg tgacatatga gaaaggattt caacatcgac
     4741 ggaaaatatg tagtgctgtc tgtaagcact aatattcagt cgccagccgt cattgtcact
     4801 gtaaagctga gcgatagaat gcctgatatt gactcaatat ccgttgcgtt tcctgtcaaa
     4861 agtatgcgta gtgctgaaca tttcgtgatg aatgccaccg aggaagaagc acggcgcggt
     4921 tttgctaaag tgatgtctga gtttggcgaa ctcttgggta aggttggaat tgtcgacctc
     4981 gagtcatgta attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa
     5041 ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta
     5101 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg
     5161 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg
     5221 ctttaatttg cggccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5281 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5341 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5401 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt
     5461 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5521 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5581 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5641 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5701 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5761 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5821 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5881 aacgtttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5941 cgcataggca agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct
     6001 tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac
     6061 ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg
     6121 gcgtcagtcc accagctaac ataaaatgta agctttcggg gctctcttgc cttccaaccc
     6181 agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca
     6241 agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg
     6301 gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact
     6361 catctccatg cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct
     6421 ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat
     6481 atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat
     6541 tgggcacaca tataataccc agcaagtcag catcggaatc tagagcacat tctgcggcct
     6601 ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa
     6661 cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc
     6721 accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttaatc
     6781 ggcaaaaaaa gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct atttttcaat
     6841 aaagaatatc ttccactact gccatctggc gtcataactg caaagtacac atatattacg
     6901 atgctgtcta ttaaatgctt cctatattat atatatagta atgtcgttta tggtgcactc
     6961 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga
//
