LOCUS       sequence-323466-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-inducible Cre recombinase (LiCre) lentiviral expression 
            plasmid for light-induced Cre/Lox recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Duplus-Bottin H, Spichty M, Triqueneaux G, Place C, Mangeot PE, 
            Ohlmann T, Vittoz F, Yvert G
  TITLE     A single-chain and fast-responding light-inducible Cre recombinase 
            as a novel optogenetic switch.
  JOURNAL   Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268.
  PUBMED    33620312
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-23"; volume: "10. pii: 61268. doi"; pages: " 
            10.7554/eLife.61268"
COMMENT     Sequence Label: pGY577
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        760..1139
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1140..1343
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2995..3374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3375..3578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3624..3644
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3648..3668
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3672..3692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3705..3722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5125..5713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5178..5198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5596..5607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(6514..6532)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6553..6569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6553..6569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6566..6588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6577..6593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6601..6607,6608..6625,6626..6631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6646..6667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6784..6801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6955..7543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7035..7054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7714..8505,8506..8574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8337..8356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8575..8679)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaattcccat tgcatacgtt gtatccatat cataatatgt
      721 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
      781 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      841 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      901 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      961 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1021 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1081 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1141 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1201 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1261 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1321 gggaggtcta tataagcaga gctcgtttag tgaaccgcag tcgctctgcg gagaggctgg
     1381 cagattgagc cctgggaggt tctctccagc actagcaggt agagcctggg tgttccctgc
     1441 tagactctca ccagcacttg gccagtgctg ggcagagtgg ctccacgctt gcttgcttaa
     1501 agacctcttc aataaagctg ccattttaga agtaagccag tgtgtgttcc catctctcct
     1561 agtcgccgcc tggtcaactc ggtactcggt aataagaaga ccctggtctg ttaggaccct
     1621 ttctgctttg agaaaccgaa gcaggaaaat ccctagcaga ttggcgcccg aacaggactt
     1681 gaaggagagt gagagactcc tgagtacggc tgagtgaagg cagtaagggc ggcaggaacc
     1741 aaccacgacg gagtgctcct ataaaggcgc gggtcggtac cagacggcgt gaggagcggg
     1801 agaggaggag gcctccggtt gcaggtaagt gcaacacaaa aaagaaatag ctgtcttgtt
     1861 atccaggaag ggataataag atagagtggg agatgggcgc gagaaactcc gtcttgtcag
     1921 ggaagaaagc agatgaattg aaaaaattat taatcgcatg aattttaaaa gaaggggagg
     1981 aataggggat atgactccag cagaaagatt aattaacatg atcactacag aacaagaaat
     2041 acaatttcaa caatcaaaaa actcaaaatt taaaaatttt cgggtctatt acagagctca
     2101 cgcgtgattg gagttgggag attataaatt agtagagatc actccgattg gcttggcccc
     2161 cacagatgtg aagaggtaca ctactggtgg cacctcaaga aataaaagag gggtctttgt
     2221 gctagggttc ttgggttttc tcgcaacggc aggttctgca atgggcgcgg cgtcgttgac
     2281 gctgaccgct cagtcccgga ctttattggc tgggatagtg cagcaacagc aacagctgtt
     2341 ggacgtggtc aagagacaac aagaattgtt gcgactgacc gtctggggaa caaagaacct
     2401 ccagactagg gtcactgcca tcgagaagta cttaaaggac caggcgcagc taaatgcttg
     2461 gggatgtgcg tttagacaag tctgccacac tactgtacca tggccaaatg caagtctaac
     2521 accagactgg aacaatgata cttggcaaga gtgggagcga aaggttgact tcttggagga
     2581 aaatataaca gccctcctag aagaggcaca aattcaacaa gagaagaaca tgtatgaatt
     2641 acaaaagttg aatagctggg atgtgtttgg caattggttt gaccttgctt cttggataaa
     2701 gtatatacaa tatggaattt atgtagttgt aggagtaata ctgttaagaa tagtgatcta
     2761 tatagtacaa atgctagcta agttaaggca ggggtatagg ccagtgttct cttccccacc
     2821 ctcttatttc cagtagactc atacccaaca ggacccggca ctgccaacca gagaaggcaa
     2881 agaaggagac ggtggagaag gcggtggatc ctattatcga tccattgcat acgttgtatc
     2941 catatcataa tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt
     3001 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3061 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3361 tcgctattac catggtgatg cggttttggc agtacgtcaa tgggcgtgga tagcggtttg
     3421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     3481 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3541 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcc
     3601 agctggctac cggtaccatg ggtccaaaaa agaagagaaa ggtagatcca aaaaagaaga
     3661 gaaaggtaga tccaaaaaag aagagaaagg taatgagggg cagccaccac caccaccacc
     3721 acggcagcct ggccaccacc ctggagagga tcgagaagaa cttcgtgatc accgacccca
     3781 ggctgcccga caaccccatc atcttcgcca gcgacagctt cctgcagctg accgagtaca
     3841 gcagggagga gatcctgggc aggaactgca ggttcctgca gggccccgag accgacaggg
     3901 ccaccgtgag gaagatcagg gacgccatcg acaaccagac cgaggtgacc gtgcagctga
     3961 tcaactacac caagagcggc aagaagttct ggaacgtgtt ccacctgcag cccatgaggg
     4021 actacaaggg cgacgtgcag tacttcatcg gcgtgcagct ggacggcacc gagaggctgc
     4081 acggcgccgc cgagagggag gccgtgtgcc tgatcaagaa gaccgccttc cagatcgaca
     4141 gcgacgaggt gaggaagaac ctgatggaca tgttcaggga caggcaggcc ttcagcgagc
     4201 acacctggaa gatgctgctg agcgtgtgca ggagctgggc cgcctggtgc aagctgaaca
     4261 acaggaagtg gttccccgcc gagcccgagg acgtgaggga ctacctgctg tacctgcagg
     4321 ccaggggcct ggccgtgaag accatccagc agcacctggg ccagctgaac atgctgcaca
     4381 ggaggagcgg cctgcccagg cccagcgaca gcaacgccgt gagcctggtg atgaggagga
     4441 tcaggaagga gaacgtggac gccggcgaga gggccaagca ggccctggcc ttcgagagga
     4501 ccgacttcga ccaggtgagg agcctgatgg agaacagcga caggtgccag gacatcagga
     4561 acctggcctt cctgggcatc gcctacaaca ccctgctgag gatcgccgag atcgccagga
     4621 tcagggtgaa ggacatcagc aggaccgacg gcggcaggat gctgatccac atcggcagga
     4681 ccaagaccct ggtgagcacc gccggcgtgg agaaggccct gagcctgggc gtgaccaagc
     4741 tggtggagag gtggatcagc gtgagcggcg tggccgacga ccccaacaac tacctgttct
     4801 gcagggtgag gaagaacggc gtggccgccc ccagcgccac cagccagctg agcaccaggg
     4861 ccctggaggg catcttcgag gccacccaca ggctgatcta cggcgccaag gacgacagcg
     4921 gccagaggta cctggcctgg agcggccaca gcgccagggt gggcgccgcc agggacatgg
     4981 ccagggccgg cgtgagcatc cccgagatca tgcaggccgg cggctggacc aacgtgaaca
     5041 tcgtgatgaa ctacatcagg aacctggaca gcgagaccgg cgccatggtg aggctgctgg
     5101 ccgccggcga ctaagcttgg atccaatcaa cctctggatt acaaaatttg tgaaagattg
     5161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5521 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5701 gccgcctccc cgcctgtttc gcctcgcgac tcgagcaaac tcacactcaa gtttatttgc
     5761 aaactcacac tcaagtttat ttgcaaactc acactcaagt catgcatggt cgagttttat
     5821 aaaagaaaag gggggactgg aagggattta ttacagtgca agaagacata gaatcttaga
     5881 catgtactta gaaaaggaag aaggcatcat accagattgg caggattaca cctcaggacc
     5941 aggaattaga tacccaaaga catttggctg gctatggaaa ttagtccctg taaatgtatc
     6001 agatgaggca caggaggatg agaggcatta tttaatgcag ccagctcaaa cttccaagtg
     6061 ggatgaccct tggggagagg ttctagcgtg gaagtttgat ccaactctag cctacactta
     6121 tgaggcatat gctagatacc cagaagagtt ggaagcaagt caggcctgtc agaactgcat
     6181 ttcgctctgt attcagtcgc tctgcggaga ggctggcaga ttgagccctg ggaggttctc
     6241 tccagcacta gcaggtagag cctgggtgtt ccctgctaga ctctcaccag cacttggcca
     6301 gtgctgggca gagtggctcc acgcttgctt gcttaaagac ctcttcaata aagctgccat
     6361 tttagaagta agccagtgtg tgttcccatc tctcctagtc gccgcctggt caactcggta
     6421 ctcggtaata agaagaccct ggtctgttag gaccctttct gctttgagaa accgaagcag
     6481 gaaaatccct agcatggtac ccagcttttg ttccctttag tgagggttaa ttccgagctt
     6541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8701 acctg
//
