LOCUS       sequence-32349-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPnlsLexA::p65Uw
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(158..409,410..763)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 agcatcagtt gtgaatgaat gttcgagccg agcagacgtg ccgctgcctt cgttaatatc
       61 ctttgaataa gccaactttg aatcacaaga cgcataccaa acggtaccgc tagcaatcaa
      121 aatgccaccc aagaagaagc gaaaagtaga agatccaatg aaggctctca cggcccgaca
      181 acaggaagtt tttgatttga tacgggatca tatatcccaa acgggtatgc ctccgacccg
      241 cgcagagata gcacagcgac tgggctttcg atcgcctaac gccgcggagg agcacttgaa
      301 ggcactggcc cgcaagggtg tcattgaaat cgtgtccggt gcgagccgcg gaatccggct
      361 gttgcaggag gaggaagagg gcctgccact ggtgggacgc gtggccgctg gcgagccgct
      421 gctggcccag caacacatag agggacacta tcaggtggac ccctccttgt ttaagccaaa
      481 tgctgatttc ctgttgcggg tgtcgggaat gtccatgaag gacatcggta ttatggatgg
      541 tgacctcctc gccgtccata agacacagga tgtcaggaac ggccaggtag tcgttgccag
      601 gatagacgat gaggtcactg tgaaacgtct caagaagcaa ggcaataagg tcgagctgct
      661 gccggagaat agcgagttca agccgatcgt ggtggatctg cgacagcagt cctttactat
      721 cgagggcttg gccgtgggtg tgatccgcaa cggagattgg ctgggatcca cgccgatgga
      781 gttccagtac ctgcccgata cggatgaccg tcaccgtatc gaagaaaagc ggaagcgaac
      841 ctatgaaacc ttcaagtcca tcatgaaaaa gtcccccttc tcgggcccca cggacccgcg
      901 ccccccgcc
//
