LOCUS       sequence-323510-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expressing PRG(RH)-Nluc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pcDNA3.1-mas-PRGRH-Nluc
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             670..1185
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 atggggagta gcaagagcaa gacgtcgaat tccggaatca agcttctgat tattggccca
       61 gaggaagatt atgacccagg ttatttcaac aatgagagtg acatcatctt ccaagatctt
      121 gaaaaactga agtcacatcc agcttacttg gtagtttttc tacgttacat cctctctcag
      181 gcagaccctg gccccctgct tttttatttg tgttcagaag tttatcaaca gacaaatccc
      241 aaagattccc gaagtctggg gaaagacatc tggaacattt tcctggagaa aaatgcgcct
      301 ctcagagtga agatccctga gatgttgcag gctgaaattg acctacgcct gcggaacaat
      361 gaggaccctc gcaatgtgct ctgtgaagct caggaggcag tcatgctgga aatccaggag
      421 cagatcaacg actacagatc caagcgtact ctgggcctgg gcagcctcta tggtgaaaat
      481 gacctgctag gcctggatgg ggaccctctt cgagaacgcc aaatggctga gaagcagctg
      541 gctgcccttg gagatatctt gtccaaatat gaggaagatc ggagtgcccc catggacttt
      601 gctgttaata cctttatgag ccacgctggg atccgtcttc gggagtctcg aggcggatca
      661 ggcggatcca tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc
      721 tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg
      781 gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac
      841 atccatgtca tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa
      901 atttttaagg tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc
      961 acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa
     1021 ggcatcgccg tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac
     1081 aaaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc
     1141 aacggagtga ccggctggcg gctgtgcgaa cgcattctgg cgtaa
//
