LOCUS       sequence-323520-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pcDNA3.1-mas-GRK2RH-Nluc L118A
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             454..969
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 atggggagta gcaagagcaa gacgtcgaat tccggaatca agcttaagta cctggaggac
       61 cggggcgagg tgacttttga gaagatcttc tcccagaagc tggggtacct gcttttccga
      121 gacttctgcc tgaagcacct ggaggaggcc aagcccttgg tagagttcta cgaggagatc
      181 aagaaatacg agaagctgga gacagaggag gagcgcctgg tctgcagccg agagatcttc
      241 gacacgtaca tcatgaagga gctggcggcc tgctcacatc ctttctcgaa gagcgccatt
      301 gagcacgtcc agggccatct ggtgaagaag caggtgcctc cggatctctt ccagccatat
      361 attgaagaaa tttgccagaa cctccgagga gacgtgttcc agaaattcat cgagagcgat
      421 aaattcacac ggttttgcca gtggaaggga tccatggtct tcacactcga agatttcgtt
      481 ggggactggc gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg
      541 tccagtttgt ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc
      601 ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc
      661 gaccaaatgg gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac
      721 tttaaggtga tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc
      781 gactatttcg gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta
      841 acagggaccc tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc
      901 tccctgctgt tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt
      961 ctggcgtaa
//
