LOCUS       sequence-323521-        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expressing p115(RH)-Nluc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pcDNA3.1-mas-p115RH-Nluc
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             676..1191
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 atggggagta gcaagagcaa gacgtcgaat tccggaatca agcttaacag ccagttccag
       61 agcctggagc aggtgaagcg gcgcccagcc cacctcatgg ccctcctgca gcacgtggcc
      121 ctgcagtttg agccaggacc cctgctttgc tgtctgcatg ccgacatgct gggctcactg
      181 ggccccaagg aggccaagaa ggccttcctg gacttctacc acagcttcct ggagaagaca
      241 gcggttctcc gggtgccggt ccctcccaac gtcgcctttg aacttgaccg cactagggct
      301 gacctcatct ccgaggatgt ccagcggcgg ttcgtgcagg aggtggtgca aagccagcag
      361 gtagccgtgg gccggcagct ggaggacttc cgttccaagc ggctcatggg catgacgccc
      421 tgggagcagg agctggccca gctggaggct tgggttgggc gggaccgagc cagctacgag
      481 gcccgggagc ggcacgtggc ggagcggctg ctcatgcacc tggaggagat gcaacatacc
      541 atctctaccg acgaagaaaa gagtgctgcc gtggtcaacg ccattggcct gtacatgcgc
      601 caccttgggg tgcggaccaa gagtggagac aagaagtcgg ggaggaactt cttccggaaa
      661 aaggtgatgg gatccatggt cttcacactc gaagatttcg ttggggactg gcgacagaca
      721 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat
      781 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag
      841 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc
      901 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac
      961 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg
     1021 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac
     1081 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta
     1141 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgta a
//
