LOCUS       sequence-32354-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPp65ADZpUw
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(112..236)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             448..468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
       61 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
      121 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      181 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgata
      241 aacggccggc cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc
      301 tgagagcatc agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa
      361 tatcctttga ataagccaac tttgaatcac aagacgcata ccaaacggta ccaaaacatg
      421 gataaagcgg aattaattcc cgagcctcca aaaaagaaga gaaaggtcga attgggatcc
      481 acgccgatgg agttccagta cctgcccgat acggatgacc gtcaccgtat cgaagaaaag
      541 cggaagcgaa cctatgaaac cttcaagtcc atcatgaaaa agtccccctt ctcgggcccc
      601 acggacccgc gccccccgcc ccgtcgtatt gcggttcctt cgcgcagcag tgccagcgtc
      661 cccaaacccg caccgcagcc ctacccgttc acttcctccc ttagcacgat taactatgat
      721 gagttcccca cgatggtgtt ccccagtgga caaatttccc aggcatcggc actggctccg
      781 gccccaccgc aagtcctccc ccaggcgccc gctccggcac cggc
//
