LOCUS       sequence-323566-        9584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses OsTIR1 under the control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 9584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK232 (CMV-OsTIR1-PURO)
FEATURES             Location/Qualifiers
     source          1..9584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        1155..1458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1459..1662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1613..1633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1700..3427
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     polyA_signal    3601..3722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3638..3657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3692..3711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3731..4231
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4150..4168
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4253..4852
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4253..4272)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4749..4769
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4859..5083
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5086..5922
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5956..5975)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5957..5975)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5982..5999)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5982..5998)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5991..6013)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6136..6438
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6317..6336
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6787..7581
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6787..7581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6841..6860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7451..7470
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(7831..8622,8623..8691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8454..8473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8815..9403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9304..9323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9557..9574
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1141 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1201 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1261 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1321 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1381 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1441 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1501 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1561 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1621 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     1681 atccgctagc gctgccacca tgacatactt tcctgaagag gtcgtcgaac acatttttag
     1741 cttcctgcct gcacagagag atagaaacac agtgagcctg gtctgcaaag tgtggtacga
     1801 gatcgaacgc ctgagccgga gaggagtgtt cgtcggcaac tgctatgctg tgagagcagg
     1861 cagggtcgcc gctaggtttc caaatgtgcg cgcactgacc gtcaagggga aaccccactt
     1921 cgccgacttt aacctggtgc cccctgattg gggaggatac gccggccctt ggatcgaggc
     1981 agccgctcgc ggctgtcatg gactggagga actgcgcatg aagcgaatgg tggtctctga
     2041 cgaaagtctg gagctgctgg ctcggagctt ccctaggttt cgcgcactgg tgctgatttc
     2101 ttgcgaaggc ttcagcaccg atggactggc agccgtggcc tcccactgta agctgctgcg
     2161 ggagctggac ctccaggaga atgaagtgga ggatagaggc cccagatggc tgtcttgctt
     2221 cccagactca tgtaccagcc tggtgtccct gaactttgcc tgcatcaaag gcgaagtgaa
     2281 tgctgggtcc ctggagcggc tggtctcaag aagccccaac ctgaggtctc tgcggctgaa
     2341 ccggagcgtg agcgtggaca ctctggctaa gattctgctg agaaccccta acctggagga
     2401 tctgggaacc ggcaatctga cagacgattt ccagacagaa tcctacttta aactgacttc
     2461 tgccctggag aagtgtaaaa tgctgaggag tctgtcagga ttctgggatg cttcacccgt
     2521 gtgcctgagc tttatctacc ctctgtgtgc acagctgaca ggcctgaacc tgagctatgc
     2581 accaaccctg gacgccagtg atctgacaaa gatgatctca cgctgcgtga aactccagcg
     2641 actgtgggtg ctggactgta tttccgataa ggggctccag gtggtcgcca gctcctgcaa
     2701 ggacctccag gagctgagag tgttcccatc tgatttttac gtggccggat atagtgctgt
     2761 cactgaggaa ggcctggtgg cagtctcact gggatgccca aagctgaaca gcctgctgta
     2821 tttctgtcat cagatgacta atgctgcact ggtgaccgtc gccaagaact gccctaattt
     2881 cacccgattt cggctgtgta ttctggaacc aggcaaaccc gacgtggtca catcccagcc
     2941 actggatgaa gggtttggag ctatcgtgag agagtgcaag ggactccaga ggctgagcat
     3001 ttccggcctg ctgacagaca aagtgttcat gtacatcggc aagtatgcta agcagctgga
     3061 gatgctgagc attgcatttg ccggagactc cgataagggc atgatgcacg tgatgaacgg
     3121 gtgtaagaat ctgcgaaaac tggaaatccg ggacagccct ttcggggatg ccgctctgct
     3181 gggaaacttt gccagatacg agacaatgag gagcctgtgg atgtctagtt gcaatgtgac
     3241 tctgaagggc tgtcaggtcc tggctagtaa aatgcctatg ctgaacgtgg aagtcattaa
     3301 tgagcgggac gggtctaacg aaatggagga aaatcatggc gacctgccaa aggtggagaa
     3361 actgtatgtg tatcggacca ccgcaggggc aagagatgat gctcccaact ttgtgaagat
     3421 tctgtgagga tccatcggat cccgggcccg tcgacggtac cgcgggcccg ggatccaccg
     3481 gggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     3541 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3721 taagttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     3781 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     3841 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     3901 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     3961 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     4021 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     4081 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     4141 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     4201 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
     4261 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     4321 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     4381 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     4441 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     4501 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     4561 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     4621 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     4681 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     4741 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     4801 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaggtaccct
     4861 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4921 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4981 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5041 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggttactag ggacaggatt
     5101 ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat attgggtcta
     5161 acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt tcctggagcc
     5221 atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag aggatcctgg
     5281 gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg cccggttctc
     5341 agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg gctgtctggt
     5401 gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag aagcagtttg
     5461 gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc acctttctag
     5521 tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag gccagtagcc
     5581 agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa actccctttg
     5641 tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc ctttctcttt
     5701 ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc ctccgcccag
     5761 agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt ccttggcaag
     5821 cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct catgcccctg
     5881 gctctcctgc cccttcccta caggggttcc tggctctgct ctaagggcga attcgcggcc
     5941 gctaaattca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     6001 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6061 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6121 agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     6181 tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt gatccccctg
     6241 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     6301 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     6361 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     6421 atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt cacctagatc
     6481 cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     6541 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     6601 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     6661 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     6721 ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg
     6781 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6841 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6901 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6961 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7021 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7081 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7141 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7201 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7261 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     7321 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7381 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7441 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7501 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7561 cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg gtattttctc
     7621 cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg
     7681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagatt
     7741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgaccaaaa tcccttaacg
     8761 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8941 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
