LOCUS       sequence-323574-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of R35L CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 R35L-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    845..1078
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1263..1307
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1574..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2026..2050
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5635..5637,5638..5640,5641..6354)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5635..5637,5638..5640,5641..6351)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5680..5701)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5941..5960)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6288..6309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6368..6956
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6421..6441)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6839..6850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7043..7276
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7348..7469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7385..7404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7439..7458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7509..7644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7664..7683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7665..7683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7693..7710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7693..7709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7702..7724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7851..8306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7938..7957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8148..8169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8332..8436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8437..8505,8506..9297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8655..8674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9468..10056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9957..9976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10210..10227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10344..10365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10380..10385,10386..10403,10404..10410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10418..10434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10423..10445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10442..10458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10442..10458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10477..10497
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10479..10497
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10525..10751
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccctgat cttcagacct ggaggaggag
      721 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
      781 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
      841 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
      901 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
      961 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1021 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1081 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1141 ggagtaatga atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1201 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1261 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1321 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1381 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1441 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1501 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     1561 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1621 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     1681 ttcaaaattt tatcgacgcg taggaacaga gaaacaggag aatatgggcc aaacaggata
     1741 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagttggaac agcagaatat
     1801 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     1861 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
     1921 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     1981 cgcttctgtt cgcgcgcttc tgctccccga gctctatata agcagagctc gtttagtgaa
     2041 ccgtcagatc gcctggagac gccatccacg ctgttttgac ttccatagaa ggatctcgac
     2101 gccgccacca tgccaggagg agggccagag atggatgact acatggagac gctgaaggat
     2161 gaagaggacg ccttgtggga gaatgtggag tgtaaccggc acatgctcag cctgtatatc
     2221 aaccctgcca agctcacgcc ctacctgcgt cagtgtaagg tcattgatga gcaggatgaa
     2281 gatgaagtgc ttaatgcccc tatgctgcca tccaagatca accgagcagg ccggctgttg
     2341 gacattctac ataccaaggg gcaaaggggc tatgtggtct tcttggagag cctagaattt
     2401 tattacccag aactgtacaa actggtgact gggaaagagc ccactcggag attctccacc
     2461 attgtggtgg aggaaggcca cgagggcctc acgcacttcc tgatgaacga ggtcatcaag
     2521 ctgcagcagc agatgaaggc caaggacctg caacgctgcg agctgctggc caggttgcgg
     2581 cagctggagg atgagaagaa gcagatgacg ctgacgcgcg tggagctgct aaccttccag
     2641 gagcggtact acaagatgaa ggaagagcgg gacagctaca atgacgagct ggtcaaggtg
     2701 aaggacgaca actacaactt agccatgcgc tacgcacagc tcagtgagga gaagaacatg
     2761 gcggtcatga ggagccgaga cctccaactc gagatcgatc agctaaagca ccggttgaat
     2821 aagatggagg aggaatgtaa gctggagaga aatcagtctc taaaactgaa gaatgacatt
     2881 gaaaatcggc ccaagaagga gcaggttctg gaactggagc gggagaatga aatgctgaag
     2941 accaaaaacc aggagctgca gtccatcatc caggccggga agcgcagcct gccagactca
     3001 gacaaggcca tcctggacat cttggaacac gaccgcaagg aggccctgga ggacaggcag
     3061 gagctggtca acaggatcta caacctgcag gaggaggccc gccaggcaga ggagctgcga
     3121 gacaagtacc tggaggagaa ggaggacctg gagctcaagt gctcgaccct gggaaaggac
     3181 tgtgaaatgt acaagcaccg catgaacacg gtcatgctgc agctggagga ggtggagcgg
     3241 gagcgggacc aggccttcca ctcccgagat gaagctcaga cacagtactc gcagtgctta
     3301 atcgaaaagg acaagtacag gaagcagatc cgcgagctgg aggagaagaa cgatgagatg
     3361 aggatcgaga tggtgcggcg ggaggcctgc atcgtcaacc tggagagcaa gctgcggcgc
     3421 ctctccaagg acagcaacaa cctggaccag agtctgccca ggaacctgcc agtaaccatc
     3481 atctctcagg actttgggga tgccagcccc aggaccaatg gtcaagaagc tgacgattct
     3541 tccacctcgg aggagtcacc tgaagacagc aagtacttcc tgccctacca tccgccccag
     3601 cgcaggatga acctgaaggg catccagctg cagagagcca aatcccccat cagcctgaag
     3661 cgaacatcag attttcaagc caaggggcac gaggaagaag gcacggatgc cagccctagc
     3721 tcctgcggat ctctgcccat caccaactcc ttcaccaaga tgcagccccc ccggagccgc
     3781 agcagcatca tgtcaatcac cgccgagccc ccgggaaacg actccatcgt cagacgctac
     3841 aaggaggacg cgccccatcg cagcacagtc gaagaagaca atgacagcgg cgggtttgac
     3901 gccttagatc tggatgatga cagtcacgaa cgctactcct tcggaccctc ctccatccac
     3961 tcctcctcct cctcccacca atccgagggc ctggatgcct acgacctgga gcaggtcaac
     4021 ctcatgttca ggaagttctc tctggaaaga cccttccggc cttcggtcac ctctgtgggg
     4081 cacgtgcggg gcccagggcc ctcggtgcag cacacgacgc tgaatggcga cagcctcacc
     4141 tcccagctca ccctgctggg gggcaacgcg cgagggagct tcgtgcactc ggtcaagcct
     4201 ggctctctgg ccgagaaagc cggcctccgt gagggccacc agctgctgct gctagaaggc
     4261 tgcatccgag gcgagaggca gagtgtcccg ttggacacat gcaccaaaga ggaagcccac
     4321 tggaccatcc agaggtgcag cggccccgtc acgctgcact acaaggtcaa ccacgaaggg
     4381 taccggaagc tggtgaagga catggaggac ggcctgatca catcggggga ctcgttctac
     4441 atccggctga acctgaacat ctccagccag ctggacgcct gcaccatgtc cctgaagtgt
     4501 gacgatgttg tgcacgtccg tgacaccatg taccaggaca ggcacgagtg gctgtgcgcg
     4561 cgggtcgacc ctttcacaga ccatgacctg gatatgggca ccatacccag ctacagccga
     4621 gcccagcagc tcctcctggt gaaactgcag cgcctgatgc accgaggcag ccgggaggag
     4681 gtagacggca cccaccacac cctgcgggca ctccggaaca ccctgcagcc agaagaagcg
     4741 ctttcaacaa gcgacccccg ggtcagcccc cgtctctcgc gagcaagctt cctttttggc
     4801 cagctccttc agttcgtcag caggtccgag aacaagtata agcggatgaa cagcaacgag
     4861 cgggtccgca tcatctcggg gagtccgcta gggagcctgg cccggtcctc gctggacgcc
     4921 accaagctct tgactgagaa gcaggaagag ctggaccctg agagcgagct gggcaagaac
     4981 ctcagcctca tcccctacag cctggtacgc gccttctact gcgagcgccg ccggcccgtg
     5041 ctcttcacac ccaccgtgct ggccaagacg ctggtgcaga ggctgctcaa ctcgggaggt
     5101 gccatggagt tcaccatctg caagtcagat atcgtcacaa gagatgagtt cctcagaagg
     5161 cagaagacgg agaccatcat ctactcccga gagaagaacc ccaacgcgtt cgaatgcatc
     5221 gcccctgcca acattgaagc tgtggccgcc aagaacaagc actgcctgct ggaggctggg
     5281 atcggctgca caagagactt gatcaagtcc aacatctacc ccatcgtgct cttcatccgg
     5341 gtgtgtgaga agaacatcaa gaggttcaga aagctgctgc cccgacctga gacggaggag
     5401 gagttcctgc gcgtgtgccg gctgaaggag aaggagctgg aggccctgcc gtgcctgtac
     5461 gccacggtgg aacctgacat gtggggcagc gtagaggagc tgctccgcgt tgtcaaggac
     5521 aagatcggcg aggagcagcg caagaccatc tgggtggacg aggaccagct gggatccggt
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatccggg ccccatggtg
     5641 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5701 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5761 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5821 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5881 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5941 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     6001 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     6061 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     6121 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     6181 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     6241 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     6301 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtgaatctag
     6361 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6421 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6481 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6541 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6601 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6661 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6721 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6781 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6841 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6901 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6961 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7021 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7081 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7141 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7201 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7261 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7321 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7441 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7621 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     7681 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7741 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7801 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     7861 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7921 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7981 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8041 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8101 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8161 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8221 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8281 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8641 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8941 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10081 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10141 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10201 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10261 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10321 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10381 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10441 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    10501 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
    10561 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
    10621 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
    10681 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
    10741 gatacataaa c
//
