LOCUS       sequence-323575-       10613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CARD domain variant with a deletion of exon 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 delta e4-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    664..897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1082..1126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1393..1510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1845..1869
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5262..5315
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5316..5318,5319..5321,5322..6035)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5316..5318,5319..5321,5322..6032)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5361..5382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5622..5641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5969..5990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6049..6637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6102..6122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6520..6531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6724..6957
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7029..7150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7066..7085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7120..7139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7190..7325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7345..7364)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7346..7364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7374..7391)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7374..7390)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7383..7405)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7532..7987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7619..7638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7829..7850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8013..8117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8118..8186,8187..8978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8336..8355)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9149..9737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9638..9657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9891..9908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10025..10046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10061..10066,10067..10084,10085..10091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10099..10115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10104..10126
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10123..10139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10123..10139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10158..10178
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10160..10178
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10206..10432
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10433..10613
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccctgatc ttcagacctg gaggaggaga
      541 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
      601 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
      661 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
      721 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
      781 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
      841 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
      901 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
      961 gagtaatgaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1021 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1081 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1141 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1201 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1261 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1321 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     1381 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1441 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     1501 tcaaaatttt atcgacgcgt aggaacagag aaacaggaga atatgggcca aacaggatat
     1561 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agttggaaca gcagaatatg
     1621 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     1681 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     1741 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     1801 gcttctgttc gcgcgcttct gctccccgag ctctatataa gcagagctcg tttagtgaac
     1861 cgtcagatcg cctggagacg ccatccacgc tgttttgact tccatagaag gatctcgacg
     1921 ccgccaccat gccaggagga gggccagaga tggatgacta catggagacg ctgaaggatg
     1981 aagaggacgc cttgtgggag aatgtggagt gtaaccggca catgctcagc cgctatatca
     2041 accctgccaa gctcacgccc tacctgcgtc agtgtaaggt cattgatgag caggatgaag
     2101 atgaagtgct taatgcccct atgctgccat ccaagatcaa ccgagcagtg gaggaaggcc
     2161 acgagggcct cacgcacttc ctgatgaacg aggtcatcaa gctgcagcag cagatgaagg
     2221 ccaaggacct gcaacgctgc gagctgctgg ccaggttgcg gcagctggag gatgagaaga
     2281 agcagatgac gctgacgcgc gtggagctgc taaccttcca ggagcggtac tacaagatga
     2341 aggaagagcg ggacagctac aatgacgagc tggtcaaggt gaaggacgac aactacaact
     2401 tagccatgcg ctacgcacag ctcagtgagg agaagaacat ggcggtcatg aggagccgag
     2461 acctccaact cgagatcgat cagctaaagc accggttgaa taagatggag gaggaatgta
     2521 agctggagag aaatcagtct ctaaaactga agaatgacat tgaaaatcgg cccaagaagg
     2581 agcaggttct ggaactggag cgggagaatg aaatgctgaa gaccaaaaac caggagctgc
     2641 agtccatcat ccaggccggg aagcgcagcc tgccagactc agacaaggcc atcctggaca
     2701 tcttggaaca cgaccgcaag gaggccctgg aggacaggca ggagctggtc aacaggatct
     2761 acaacctgca ggaggaggcc cgccaggcag aggagctgcg agacaagtac ctggaggaga
     2821 aggaggacct ggagctcaag tgctcgaccc tgggaaagga ctgtgaaatg tacaagcacc
     2881 gcatgaacac ggtcatgctg cagctggagg aggtggagcg ggagcgggac caggccttcc
     2941 actcccgaga tgaagctcag acacagtact cgcagtgctt aatcgaaaag gacaagtaca
     3001 ggaagcagat ccgcgagctg gaggagaaga acgatgagat gaggatcgag atggtgcggc
     3061 gggaggcctg catcgtcaac ctggagagca agctgcggcg cctctccaag gacagcaaca
     3121 acctggacca gagtctgccc aggaacctgc cagtaaccat catctctcag gactttgggg
     3181 atgccagccc caggaccaat ggtcaagaag ctgacgattc ttccacctcg gaggagtcac
     3241 ctgaagacag caagtacttc ctgccctacc atccgcccca gcgcaggatg aacctgaagg
     3301 gcatccagct gcagagagcc aaatccccca tcagcctgaa gcgaacatca gattttcaag
     3361 ccaaggggca cgaggaagaa ggcacggatg ccagccctag ctcctgcgga tctctgccca
     3421 tcaccaactc cttcaccaag atgcagcccc cccggagccg cagcagcatc atgtcaatca
     3481 ccgccgagcc cccgggaaac gactccatcg tcagacgcta caaggaggac gcgccccatc
     3541 gcagcacagt cgaagaagac aatgacagcg gcgggtttga cgccttagat ctggatgatg
     3601 acagtcacga acgctactcc ttcggaccct cctccatcca ctcctcctcc tcctcccacc
     3661 aatccgaggg cctggatgcc tacgacctgg agcaggtcaa cctcatgttc aggaagttct
     3721 ctctggaaag acccttccgg ccttcggtca cctctgtggg gcacgtgcgg ggcccagggc
     3781 cctcggtgca gcacacgacg ctgaatggcg acagcctcac ctcccagctc accctgctgg
     3841 ggggcaacgc gcgagggagc ttcgtgcact cggtcaagcc tggctctctg gccgagaaag
     3901 ccggcctccg tgagggccac cagctgctgc tgctagaagg ctgcatccga ggcgagaggc
     3961 agagtgtccc gttggacaca tgcaccaaag aggaagccca ctggaccatc cagaggtgca
     4021 gcggccccgt cacgctgcac tacaaggtca accacgaagg gtaccggaag ctggtgaagg
     4081 acatggagga cggcctgatc acatcggggg actcgttcta catccggctg aacctgaaca
     4141 tctccagcca gctggacgcc tgcaccatgt ccctgaagtg tgacgatgtt gtgcacgtcc
     4201 gtgacaccat gtaccaggac aggcacgagt ggctgtgcgc gcgggtcgac cctttcacag
     4261 accatgacct ggatatgggc accataccca gctacagccg agcccagcag ctcctcctgg
     4321 tgaaactgca gcgcctgatg caccgaggca gccgggagga ggtagacggc acccaccaca
     4381 ccctgcgggc actccggaac accctgcagc cagaagaagc gctttcaaca agcgaccccc
     4441 gggtcagccc ccgtctctcg cgagcaagct tcctttttgg ccagctcctt cagttcgtca
     4501 gcaggtccga gaacaagtat aagcggatga acagcaacga gcgggtccgc atcatctcgg
     4561 ggagtccgct agggagcctg gcccggtcct cgctggacgc caccaagctc ttgactgaga
     4621 agcaggaaga gctggaccct gagagcgagc tgggcaagaa cctcagcctc atcccctaca
     4681 gcctggtacg cgccttctac tgcgagcgcc gccggcccgt gctcttcaca cccaccgtgc
     4741 tggccaagac gctggtgcag aggctgctca actcgggagg tgccatggag ttcaccatct
     4801 gcaagtcaga tatcgtcaca agagatgagt tcctcagaag gcagaagacg gagaccatca
     4861 tctactcccg agagaagaac cccaacgcgt tcgaatgcat cgcccctgcc aacattgaag
     4921 ctgtggccgc caagaacaag cactgcctgc tggaggctgg gatcggctgc acaagagact
     4981 tgatcaagtc caacatctac cccatcgtgc tcttcatccg ggtgtgtgag aagaacatca
     5041 agaggttcag aaagctgctg ccccgacctg agacggagga ggagttcctg cgcgtgtgcc
     5101 ggctgaagga gaaggagctg gaggccctgc cgtgcctgta cgccacggtg gaacctgaca
     5161 tgtggggcag cgtagaggag ctgctccgcg ttgtcaagga caagatcggc gaggagcagc
     5221 gcaagaccat ctgggtggac gaggaccagc tgggatccgg tgagggcaga ggaagtcttc
     5281 taacatgcgg tgacgtggag gagaatccgg gccccatggt gagcaagggc gaggagctgt
     5341 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     5401 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     5461 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     5521 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     5581 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     5641 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     5701 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5761 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     5821 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5881 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5941 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     6001 ggatcactct cggcatggac gagctgtaca agtgaatcta gagtcgacaa tcaacctctg
     6061 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6121 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6181 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6241 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6301 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6361 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6421 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6481 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6541 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6601 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     6661 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6721 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6781 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6841 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6901 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6961 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7021 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7141 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     7201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7261 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7321 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7381 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7441 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7501 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7741 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7861 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7981 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8041 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8101 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8161 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8221 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8281 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8341 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8401 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8461 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8521 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8581 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8641 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8701 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8761 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8821 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8881 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8941 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9001 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9061 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9541 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9781 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9841 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9901 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9961 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10021 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10081 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10141 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    10201 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
    10261 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
    10321 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
    10381 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
    10441 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10501 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10561 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gca
//
