LOCUS       sequence-323578-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of R30W CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 R30W-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3557..3610
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3611..3613,3614..3616,3617..4330)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3611..3613,3614..3616,3617..4327)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3656..3677)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3917..3936)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4264..4285
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4344..4932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4397..4417)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4815..4826)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5019..5252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5324..5445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5361..5380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5415..5434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5485..5620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5640..5659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5641..5659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5669..5686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5669..5685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5678..5700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5827..6282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5914..5933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6124..6145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6308..6412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6413..6481,6482..7273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6631..6650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7444..8032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7933..7952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8186..8203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8320..8341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8356..8361,8362..8379,8380..8386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8394..8410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8399..8421
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8418..8434
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8418..8434
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8453..8473
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8455..8473
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8501..8727
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8728..8908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8955..9080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9572..9805
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9990..10034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10301..10418
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacttcc
       61 atagaaggat ctcgacgccg ccaccatgcc aggaggaggg ccagagatgg atgactacat
      121 ggagacgctg aaggatgaag aggacgcctt gtgggagaat gtggagtgta actggcacat
      181 gctcagccgc tatatcaacc ctgccaagct cacgccctac ctgcgtcagt gtaaggtcat
      241 tgatgagcag gatgaagatg aagtgcttaa tgcccctatg ctgccatcca agatcaaccg
      301 agcaggccgg ctgttggaca ttctacatac caaggggcaa aggggctatg tggtcttctt
      361 ggagagccta gaattttatt acccagaact gtacaaactg gtgactggga aagagcccac
      421 tcggagattc tccaccattg tggtggagga aggccacgag ggcctcacgc acttcctgat
      481 gaacgaggtc atcaagctgc agcagcagat gaaggccaag gacctgcaac gctgcgagct
      541 gctggccagg ttgcggcagc tggaggatga gaagaagcag atgacgctga cgcgcgtgga
      601 gctgctaacc ttccaggagc ggtactacaa gatgaaggaa gagcgggaca gctacaatga
      661 cgagctggtc aaggtgaagg acgacaacta caacttagcc atgcgctacg cacagctcag
      721 tgaggagaag aacatggcgg tcatgaggag ccgagacctc caactcgaga tcgatcagct
      781 aaagcaccgg ttgaataaga tggaggagga atgtaagctg gagagaaatc agtctctaaa
      841 actgaagaat gacattgaaa atcggcccaa gaaggagcag gttctggaac tggagcggga
      901 gaatgaaatg ctgaagacca aaaaccagga gctgcagtcc atcatccagg ccgggaagcg
      961 cagcctgcca gactcagaca aggccatcct ggacatcttg gaacacgacc gcaaggaggc
     1021 cctggaggac aggcaggagc tggtcaacag gatctacaac ctgcaggagg aggcccgcca
     1081 ggcagaggag ctgcgagaca agtacctgga ggagaaggag gacctggagc tcaagtgctc
     1141 gaccctggga aaggactgtg aaatgtacaa gcaccgcatg aacacggtca tgctgcagct
     1201 ggaggaggtg gagcgggagc gggaccaggc cttccactcc cgagatgaag ctcagacaca
     1261 gtactcgcag tgcttaatcg aaaaggacaa gtacaggaag cagatccgcg agctggagga
     1321 gaagaacgat gagatgagga tcgagatggt gcggcgggag gcctgcatcg tcaacctgga
     1381 gagcaagctg cggcgcctct ccaaggacag caacaacctg gaccagagtc tgcccaggaa
     1441 cctgccagta accatcatct ctcaggactt tggggatgcc agccccagga ccaatggtca
     1501 agaagctgac gattcttcca cctcggagga gtcacctgaa gacagcaagt acttcctgcc
     1561 ctaccatccg ccccagcgca ggatgaacct gaagggcatc cagctgcaga gagccaaatc
     1621 ccccatcagc ctgaagcgaa catcagattt tcaagccaag gggcacgagg aagaaggcac
     1681 ggatgccagc cctagctcct gcggatctct gcccatcacc aactccttca ccaagatgca
     1741 gcccccccgg agccgcagca gcatcatgtc aatcaccgcc gagcccccgg gaaacgactc
     1801 catcgtcaga cgctacaagg aggacgcgcc ccatcgcagc acagtcgaag aagacaatga
     1861 cagcggcggg tttgacgcct tagatctgga tgatgacagt cacgaacgct actccttcgg
     1921 accctcctcc atccactcct cctcctcctc ccaccaatcc gagggcctgg atgcctacga
     1981 cctggagcag gtcaacctca tgttcaggaa gttctctctg gaaagaccct tccggccttc
     2041 ggtcacctct gtggggcacg tgcggggccc agggccctcg gtgcagcaca cgacgctgaa
     2101 tggcgacagc ctcacctccc agctcaccct gctggggggc aacgcgcgag ggagcttcgt
     2161 gcactcggtc aagcctggct ctctggccga gaaagccggc ctccgtgagg gccaccagct
     2221 gctgctgcta gaaggctgca tccgaggcga gaggcagagt gtcccgttgg acacatgcac
     2281 caaagaggaa gcccactgga ccatccagag gtgcagcggc cccgtcacgc tgcactacaa
     2341 ggtcaaccac gaagggtacc ggaagctggt gaaggacatg gaggacggcc tgatcacatc
     2401 gggggactcg ttctacatcc ggctgaacct gaacatctcc agccagctgg acgcctgcac
     2461 catgtccctg aagtgtgacg atgttgtgca cgtccgtgac accatgtacc aggacaggca
     2521 cgagtggctg tgcgcgcggg tcgacccttt cacagaccat gacctggata tgggcaccat
     2581 acccagctac agccgagccc agcagctcct cctggtgaaa ctgcagcgcc tgatgcaccg
     2641 aggcagccgg gaggaggtag acggcaccca ccacaccctg cgggcactcc ggaacaccct
     2701 gcagccagaa gaagcgcttt caacaagcga cccccgggtc agcccccgtc tctcgcgagc
     2761 aagcttcctt tttggccagc tccttcagtt cgtcagcagg tccgagaaca agtataagcg
     2821 gatgaacagc aacgagcggg tccgcatcat ctcggggagt ccgctaggga gcctggcccg
     2881 gtcctcgctg gacgccacca agctcttgac tgagaagcag gaagagctgg accctgagag
     2941 cgagctgggc aagaacctca gcctcatccc ctacagcctg gtacgcgcct tctactgcga
     3001 gcgccgccgg cccgtgctct tcacacccac cgtgctggcc aagacgctgg tgcagaggct
     3061 gctcaactcg ggaggtgcca tggagttcac catctgcaag tcagatatcg tcacaagaga
     3121 tgagttcctc agaaggcaga agacggagac catcatctac tcccgagaga agaaccccaa
     3181 cgcgttcgaa tgcatcgccc ctgccaacat tgaagctgtg gccgccaaga acaagcactg
     3241 cctgctggag gctgggatcg gctgcacaag agacttgatc aagtccaaca tctaccccat
     3301 cgtgctcttc atccgggtgt gtgagaagaa catcaagagg ttcagaaagc tgctgccccg
     3361 acctgagacg gaggaggagt tcctgcgcgt gtgccggctg aaggagaagg agctggaggc
     3421 cctgccgtgc ctgtacgcca cggtggaacc tgacatgtgg ggcagcgtag aggagctgct
     3481 ccgcgttgtc aaggacaaga tcggcgagga gcagtgcaag accatctggg tggacgagga
     3541 ccagctggga tccggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     3601 tccgggcccc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3661 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3721 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3781 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3841 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3901 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3961 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4021 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     4081 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     4141 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4201 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4261 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4321 gtacaagtga atctagagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     4381 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4441 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4501 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4561 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4621 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4681 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4741 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4801 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4861 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4921 ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc
     4981 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5041 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5101 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5161 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5221 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5281 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5461 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5581 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5641 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5701 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5761 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5821 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5881 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5941 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6001 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6061 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6121 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6181 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6241 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6421 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6481 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6541 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6601 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6661 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6721 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6781 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6841 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6901 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6961 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7021 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7081 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7141 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7201 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7261 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7321 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8101 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8161 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8221 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8281 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8341 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8401 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     8461 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     8521 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8581 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     8641 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     8701 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     8761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8881 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8941 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     9001 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     9061 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     9121 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     9181 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     9241 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     9301 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     9361 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     9421 ccctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9481 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9541 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9601 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9661 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9721 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     9781 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9841 ccactgctgt gccttggaat gctagttgga gtaatgaatc tctggaacag atttggaatc
     9901 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9961 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10021 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10081 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10141 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10201 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    10261 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
    10321 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10381 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgacgcgtag gaacagagaa
    10441 acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
    10501 ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
    10561 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
    10621 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
    10681 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
    10741 ctatataagc a
//
