LOCUS       sequence-32358-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Hardin JW, Hu YX, McKay DB
  TITLE     Structure of the RNA binding domain of a DEAD-box helicase bound to 
            its ribosomal RNA target reveals a novel mode of recognition by an 
            RNA recognition motif.
  JOURNAL   J Mol Biol. 2010 Sep 17;402(2):412-27. doi: 
            10.1016/j.jmb.2010.07.040. Epub 2010 Jul 29.
  PUBMED    20673833
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.07.040"; journalName: "J Mol Biol"; date: 
            "2010-09-17- 17"; volume: "402"; issue: "2"; pages: "412-27"
COMMENT     Sequence Label: 23SrRNA.RAV12(wt).2508-2580.G2530AAAC
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      205..252
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(271..287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(271..287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(284..306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    295..311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(319..325,326..343,344..349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(502..519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(753..772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctgttcgc catttaaagt ggtacgcgag ctagccggcc atggtcccag cctcctcgct
       61 ggcggccggt gggcaacatg cttcggcatg gcgaatggga cctctagact gtgcatcggg
      121 tcaggactga aaggtagcag ccctgggcag ttttttcctg tgcatcgggt caggaccttc
      181 gggtagcagc cctgggcagg atccctagca taaccccttg gggcctctaa acgggtcttg
      241 aggggttttt tgaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      901 gtcgttcgct ccaagctggg ctg
//
