LOCUS       sequence-323581-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of R72L CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 R72L-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             185..229
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    496..613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     948..972
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4503..4556
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4557..4559,4560..4562,4563..5276)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4557..4559,4560..4562,4563..5273)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4602..4623)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4863..4882)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5210..5231
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5290..5878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5343..5363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5761..5772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5965..6198
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6270..6391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6307..6326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6361..6380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6431..6566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6586..6605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6587..6605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6615..6632)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6615..6631)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6624..6646)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6773..7228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6860..6879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7070..7091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7254..7358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7359..7427,7428..8219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7577..7596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8390..8978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8879..8898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9132..9149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9266..9287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9302..9307,9308..9325,9326..9332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9340..9356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9345..9367
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9364..9380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9364..9380
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9399..9419
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9401..9419
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9447..9673
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9674..9854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9901..10026
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10518..10751
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
       61 ttggagtaat gaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
      121 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
      181 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
      241 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
      301 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
      361 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
      421 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
      481 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      541 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      601 aattcaaaat tttatcgacg cgtaggaaca gagaaacagg agaatatggg ccaaacagga
      661 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat
      721 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
      781 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
      841 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
      901 ctcgcttctg ttcgcgcgct tctgctcccc gagctctata taagcagagc tcgtttagtg
      961 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acttccatag aaggatctcg
     1021 acgccgccac catgccagga ggagggccag agatggatga ctacatggag acgctgaagg
     1081 atgaagagga cgccttgtgg gagaatgtgg agtgtaaccg gcacatgctc agccgctata
     1141 tcaaccctgc caagctcacg ccctacctgc gtcagtgtaa ggtcattgat gagcaggatg
     1201 aagatgaagt gcttaatgcc cctatgctgc catccaagat caacctggca ggccggctgt
     1261 tggacattct acataccaag gggcaaaggg gctatgtggt cttcttggag agcctagaat
     1321 tttattaccc agaactgtac aaactggtga ctgggaaaga gcccactcgg agattctcca
     1381 ccattgtggt ggaggaaggc cacgagggcc tcacgcactt cctgatgaac gaggtcatca
     1441 agctgcagca gcagatgaag gccaaggacc tgcaacgctg cgagctgctg gccaggttgc
     1501 ggcagctgga ggatgagaag aagcagatga cgctgacgcg cgtggagctg ctaaccttcc
     1561 aggagcggta ctacaagatg aaggaagagc gggacagcta caatgacgag ctggtcaagg
     1621 tgaaggacga caactacaac ttagccatgc gctacgcaca gctcagtgag gagaagaaca
     1681 tggcggtcat gaggagccga gacctccaac tcgagatcga tcagctaaag caccggttga
     1741 ataagatgga ggaggaatgt aagctggaga gaaatcagtc tctaaaactg aagaatgaca
     1801 ttgaaaatcg gcccaagaag gagcaggttc tggaactgga gcgggagaat gaaatgctga
     1861 agaccaaaaa ccaggagctg cagtccatca tccaggccgg gaagcgcagc ctgccagact
     1921 cagacaaggc catcctggac atcttggaac acgaccgcaa ggaggccctg gaggacaggc
     1981 aggagctggt caacaggatc tacaacctgc aggaggaggc ccgccaggca gaggagctgc
     2041 gagacaagta cctggaggag aaggaggacc tggagctcaa gtgctcgacc ctgggaaagg
     2101 actgtgaaat gtacaagcac cgcatgaaca cggtcatgct gcagctggag gaggtggagc
     2161 gggagcggga ccaggccttc cactcccgag atgaagctca gacacagtac tcgcagtgct
     2221 taatcgaaaa ggacaagtac aggaagcaga tccgcgagct ggaggagaag aacgatgaga
     2281 tgaggatcga gatggtgcgg cgggaggcct gcatcgtcaa cctggagagc aagctgcggc
     2341 gcctctccaa ggacagcaac aacctggacc agagtctgcc caggaacctg ccagtaacca
     2401 tcatctctca ggactttggg gatgccagcc ccaggaccaa tggtcaagaa gctgacgatt
     2461 cttccacctc ggaggagtca cctgaagaca gcaagtactt cctgccctac catccgcccc
     2521 agcgcaggat gaacctgaag ggcatccagc tgcagagagc caaatccccc atcagcctga
     2581 agcgaacatc agattttcaa gccaaggggc acgaggaaga aggcacggat gccagcccta
     2641 gctcctgcgg atctctgccc atcaccaact ccttcaccaa gatgcagccc ccccggagcc
     2701 gcagcagcat catgtcaatc accgccgagc ccccgggaaa cgactccatc gtcagacgct
     2761 acaaggagga cgcgccccat cgcagcacag tcgaagaaga caatgacagc ggcgggtttg
     2821 acgccttaga tctggatgat gacagtcacg aacgctactc cttcggaccc tcctccatcc
     2881 actcctcctc ctcctcccac caatccgagg gcctggatgc ctacgacctg gagcaggtca
     2941 acctcatgtt caggaagttc tctctggaaa gacccttccg gccttcggtc acctctgtgg
     3001 ggcacgtgcg gggcccaggg ccctcggtgc agcacacgac gctgaatggc gacagcctca
     3061 cctcccagct caccctgctg gggggcaacg cgcgagggag cttcgtgcac tcggtcaagc
     3121 ctggctctct ggccgagaaa gccggcctcc gtgagggcca ccagctgctg ctgctagaag
     3181 gctgcatccg aggcgagagg cagagtgtcc cgttggacac atgcaccaaa gaggaagccc
     3241 actggaccat ccagaggtgc agcggccccg tcacgctgca ctacaaggtc aaccacgaag
     3301 ggtaccggaa gctggtgaag gacatggagg acggcctgat cacatcgggg gactcgttct
     3361 acatccggct gaacctgaac atctccagcc agctggacgc ctgcaccatg tccctgaagt
     3421 gtgacgatgt tgtgcacgtc cgtgacacca tgtaccagga caggcacgag tggctgtgcg
     3481 cgcgggtcga ccctttcaca gaccatgacc tggatatggg caccataccc agctacagcc
     3541 gagcccagca gctcctcctg gtgaaactgc agcgcctgat gcaccgaggc agccgggagg
     3601 aggtagacgg cacccaccac accctgcggg cactccggaa caccctgcag ccagaagaag
     3661 cgctttcaac aagcgacccc cgggtcagcc cccgtctctc gcgagcaagc ttcctttttg
     3721 gccagctcct tcagttcgtc agcaggtccg agaacaagta taagcggatg aacagcaacg
     3781 agcgggtccg catcatctcg gggagtccgc tagggagcct ggcccggtcc tcgctggacg
     3841 ccaccaagct cttgactgag aagcaggaag agctggaccc tgagagcgag ctgggcaaga
     3901 acctcagcct catcccctac agcctggtac gcgccttcta ctgcgagcgc cgccggcccg
     3961 tgctcttcac acccaccgtg ctggccaaga cgctggtgca gaggctgctc aactcgggag
     4021 gtgccatgga gttcaccatc tgcaagtcag atatcgtcac aagagatgag ttcctcagaa
     4081 ggcagaagac ggagaccatc atctactccc gagagaagaa ccccaacgcg ttcgaatgca
     4141 tcgcccctgc caacattgaa gctgtggccg ccaagaacaa gcactgcctg ctggaggctg
     4201 ggatcggctg cacaagagac ttgatcaagt ccaacatcta ccccatcgtg ctcttcatcc
     4261 gggtgtgtga gaagaacatc aagaggttca gaaagctgct gccccgacct gagacggagg
     4321 aggagttcct gcgcgtgtgc cggctgaagg agaaggagct ggaggccctg ccgtgcctgt
     4381 acgccacggt ggaacctgac atgtggggca gcgtagagga gctgctccgc gttgtcaagg
     4441 acaagatcgg cgaggagcag cgcaagacca tctgggtgga cgaggaccag ctgggatccg
     4501 gtgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccg ggccccatgg
     4561 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4621 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4681 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4741 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4801 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4861 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4921 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4981 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5041 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5101 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5161 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5221 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgaatct
     5281 agagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5341 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5401 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5461 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5521 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5581 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5641 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5701 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5761 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5821 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5881 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5941 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6001 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6061 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6121 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6181 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6241 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6421 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6481 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6541 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6601 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6661 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6721 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6841 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6961 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7081 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7141 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7201 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9181 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9241 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9301 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9361 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9421 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9481 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9541 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9601 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9661 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9841 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9901 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9961 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10021 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10081 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10141 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10201 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10261 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10321 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccct gatcttcaga
    10381 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10441 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10501 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10561 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10621 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10681 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10741 caacagctcc t
//
