LOCUS       sequence-323582-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of R72K CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 R72K-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3558..3611
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3612..3614,3615..3617,3618..4331)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3612..3614,3615..3617,3618..4328)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3657..3678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3918..3937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4265..4286
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4345..4933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4398..4418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4816..4827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5020..5253
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5325..5446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5362..5381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5416..5435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5486..5621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5641..5660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5642..5660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5670..5687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5670..5686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5679..5701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5828..6283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5915..5934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6125..6146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6309..6413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6414..6482,6483..7274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6632..6651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7445..8033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7934..7953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8187..8204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8321..8342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8357..8362,8363..8380,8381..8387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8395..8411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8400..8422
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8419..8435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8419..8435
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8454..8474
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8456..8474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8502..8728
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8729..8909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8956..9081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9573..9806
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9991..10035
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10302..10419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacttc
       61 catagaagga tctcgacgcc gccaccatgc caggaggagg gccagagatg gatgactaca
      121 tggagacgct gaaggatgaa gaggacgcct tgtgggagaa tgtggagtgt aaccggcaca
      181 tgctcagccg ctatatcaac cctgccaagc tcacgcccta cctgcgtcag tgtaaggtca
      241 ttgatgagca ggatgaagat gaagtgctta atgcccctat gctgccatcc aagatcaaca
      301 aggcaggccg gctgttggac attctacata ccaaggggca aaggggctat gtggtcttct
      361 tggagagcct agaattttat tacccagaac tgtacaaact ggtgactggg aaagagccca
      421 ctcggagatt ctccaccatt gtggtggagg aaggccacga gggcctcacg cacttcctga
      481 tgaacgaggt catcaagctg cagcagcaga tgaaggccaa ggacctgcaa cgctgcgagc
      541 tgctggccag gttgcggcag ctggaggatg agaagaagca gatgacgctg acgcgcgtgg
      601 agctgctaac cttccaggag cggtactaca agatgaagga agagcgggac agctacaatg
      661 acgagctggt caaggtgaag gacgacaact acaacttagc catgcgctac gcacagctca
      721 gtgaggagaa gaacatggcg gtcatgagga gccgagacct ccaactcgag atcgatcagc
      781 taaagcaccg gttgaataag atggaggagg aatgtaagct ggagagaaat cagtctctaa
      841 aactgaagaa tgacattgaa aatcggccca agaaggagca ggttctggaa ctggagcggg
      901 agaatgaaat gctgaagacc aaaaaccagg agctgcagtc catcatccag gccgggaagc
      961 gcagcctgcc agactcagac aaggccatcc tggacatctt ggaacacgac cgcaaggagg
     1021 ccctggagga caggcaggag ctggtcaaca ggatctacaa cctgcaggag gaggcccgcc
     1081 aggcagagga gctgcgagac aagtacctgg aggagaagga ggacctggag ctcaagtgct
     1141 cgaccctggg aaaggactgt gaaatgtaca agcaccgcat gaacacggtc atgctgcagc
     1201 tggaggaggt ggagcgggag cgggaccagg ccttccactc ccgagatgaa gctcagacac
     1261 agtactcgca gtgcttaatc gaaaaggaca agtacaggaa gcagatccgc gagctggagg
     1321 agaagaacga tgagatgagg atcgagatgg tgcggcggga ggcctgcatc gtcaacctgg
     1381 agagcaagct gcggcgcctc tccaaggaca gcaacaacct ggaccagagt ctgcccagga
     1441 acctgccagt aaccatcatc tctcaggact ttggggatgc cagccccagg accaatggtc
     1501 aagaagctga cgattcttcc acctcggagg agtcacctga agacagcaag tacttcctgc
     1561 cctaccatcc gccccagcgc aggatgaacc tgaagggcat ccagctgcag agagccaaat
     1621 cccccatcag cctgaagcga acatcagatt ttcaagccaa ggggcacgag gaagaaggca
     1681 cggatgccag ccctagctcc tgcggatctc tgcccatcac caactccttc accaagatgc
     1741 agcccccccg gagccgcagc agcatcatgt caatcaccgc cgagcccccg ggaaacgact
     1801 ccatcgtcag acgctacaag gaggacgcgc cccatcgcag cacagtcgaa gaagacaatg
     1861 acagcggcgg gtttgacgcc ttagatctgg atgatgacag tcacgaacgc tactccttcg
     1921 gaccctcctc catccactcc tcctcctcct cccaccaatc cgagggcctg gatgcctacg
     1981 acctggagca ggtcaacctc atgttcagga agttctctct ggaaagaccc ttccggcctt
     2041 cggtcacctc tgtggggcac gtgcggggcc cagggccctc ggtgcagcac acgacgctga
     2101 atggcgacag cctcacctcc cagctcaccc tgctgggggg caacgcgcga gggagcttcg
     2161 tgcactcggt caagcctggc tctctggccg agaaagccgg cctccgtgag ggccaccagc
     2221 tgctgctgct agaaggctgc atccgaggcg agaggcagag tgtcccgttg gacacatgca
     2281 ccaaagagga agcccactgg accatccaga ggtgcagcgg ccccgtcacg ctgcactaca
     2341 aggtcaacca cgaagggtac cggaagctgg tgaaggacat ggaggacggc ctgatcacat
     2401 cgggggactc gttctacatc cggctgaacc tgaacatctc cagccagctg gacgcctgca
     2461 ccatgtccct gaagtgtgac gatgttgtgc acgtccgtga caccatgtac caggacaggc
     2521 acgagtggct gtgcgcgcgg gtcgaccctt tcacagacca tgacctggat atgggcacca
     2581 tacccagcta cagccgagcc cagcagctcc tcctggtgaa actgcagcgc ctgatgcacc
     2641 gaggcagccg ggaggaggta gacggcaccc accacaccct gcgggcactc cggaacaccc
     2701 tgcagccaga agaagcgctt tcaacaagcg acccccgggt cagcccccgt ctctcgcgag
     2761 caagcttcct ttttggccag ctccttcagt tcgtcagcag gtccgagaac aagtataagc
     2821 ggatgaacag caacgagcgg gtccgcatca tctcggggag tccgctaggg agcctggccc
     2881 ggtcctcgct ggacgccacc aagctcttga ctgagaagca ggaagagctg gaccctgaga
     2941 gcgagctggg caagaacctc agcctcatcc cctacagcct ggtacgcgcc ttctactgcg
     3001 agcgccgccg gcccgtgctc ttcacaccca ccgtgctggc caagacgctg gtgcagaggc
     3061 tgctcaactc gggaggtgcc atggagttca ccatctgcaa gtcagatatc gtcacaagag
     3121 atgagttcct cagaaggcag aagacggaga ccatcatcta ctcccgagag aagaacccca
     3181 acgcgttcga atgcatcgcc cctgccaaca ttgaagctgt ggccgccaag aacaagcact
     3241 gcctgctgga ggctgggatc ggctgcacaa gagacttgat caagtccaac atctacccca
     3301 tcgtgctctt catccgggtg tgtgagaaga acatcaagag gttcagaaag ctgctgcccc
     3361 gacctgagac ggaggaggag ttcctgcgcg tgtgccggct gaaggagaag gagctggagg
     3421 ccctgccgtg cctgtacgcc acggtggaac ctgacatgtg gggcagcgta gaggagctgc
     3481 tccgcgttgt caaggacaag atcggcgagg agcagcgcaa gaccatctgg gtggacgagg
     3541 accagctggg atccggtgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     3601 atccgggccc catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3661 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3721 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3781 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3841 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3901 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3961 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4021 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4081 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4141 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4201 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4261 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4321 tgtacaagtg aatctagagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4381 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4441 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4501 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4561 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4621 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4681 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     4741 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4801 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4861 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4921 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5041 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5101 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5161 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5221 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5281 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5461 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5521 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5581 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5641 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5821 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6241 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7561 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8461 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     8521 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     8581 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     8641 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     8701 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     8761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8881 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8941 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9001 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9061 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9121 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     9181 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9241 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     9301 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9361 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9421 gccctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9481 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9541 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9601 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9661 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9721 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9781 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9841 accactgctg tgccttggaa tgctagttgg agtaatgaat ctctggaaca gatttggaat
     9901 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9961 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    10021 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    10081 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    10141 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    10201 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    10261 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
    10321 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
    10381 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgacgcgta ggaacagaga
    10441 aacaggagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
    10501 gccaagaaca gttggaacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
    10561 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
    10621 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
    10681 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
    10741 tctatataag c
//
