LOCUS       sequence-323584-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of K69Q CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 K69Q-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3552..3605
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3606..3608,3609..3611,3612..4325)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3606..3608,3609..3611,3612..4322)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3651..3672)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3912..3931)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4259..4280
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4339..4927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4392..4412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4810..4821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5014..5247
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5319..5440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5356..5375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5410..5429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5480..5615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5635..5654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5636..5654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5664..5681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5664..5680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5673..5695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5822..6277
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5909..5928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6119..6140
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6303..6407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6408..6476,6477..7268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6626..6645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7439..8027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7928..7947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8181..8198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8315..8336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8351..8356,8357..8374,8375..8381)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8389..8405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8394..8416
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8413..8429
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8413..8429
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8448..8468
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8450..8468
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8496..8722
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8723..8903
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8950..9075
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9567..9800
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9985..10029
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10296..10413
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     join(10748..10751,1..21)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cttccataga
       61 aggatctcga cgccgccacc atgccaggag gagggccaga gatggatgac tacatggaga
      121 cgctgaagga tgaagaggac gccttgtggg agaatgtgga gtgtaaccgg cacatgctca
      181 gccgctatat caaccctgcc aagctcacgc cctacctgcg tcagtgtaag gtcattgatg
      241 agcaggatga agatgaagtg cttaatgccc ctatgctgcc atcccagatc aaccgagcag
      301 gccggctgtt ggacattcta cataccaagg ggcaaagggg ctatgtggtc ttcttggaga
      361 gcctagaatt ttattaccca gaactgtaca aactggtgac tgggaaagag cccactcgga
      421 gattctccac cattgtggtg gaggaaggcc acgagggcct cacgcacttc ctgatgaacg
      481 aggtcatcaa gctgcagcag cagatgaagg ccaaggacct gcaacgctgc gagctgctgg
      541 ccaggttgcg gcagctggag gatgagaaga agcagatgac gctgacgcgc gtggagctgc
      601 taaccttcca ggagcggtac tacaagatga aggaagagcg ggacagctac aatgacgagc
      661 tggtcaaggt gaaggacgac aactacaact tagccatgcg ctacgcacag ctcagtgagg
      721 agaagaacat ggcggtcatg aggagccgag acctccaact cgagatcgat cagctaaagc
      781 accggttgaa taagatggag gaggaatgta agctggagag aaatcagtct ctaaaactga
      841 agaatgacat tgaaaatcgg cccaagaagg agcaggttct ggaactggag cgggagaatg
      901 aaatgctgaa gaccaaaaac caggagctgc agtccatcat ccaggccggg aagcgcagcc
      961 tgccagactc agacaaggcc atcctggaca tcttggaaca cgaccgcaag gaggccctgg
     1021 aggacaggca ggagctggtc aacaggatct acaacctgca ggaggaggcc cgccaggcag
     1081 aggagctgcg agacaagtac ctggaggaga aggaggacct ggagctcaag tgctcgaccc
     1141 tgggaaagga ctgtgaaatg tacaagcacc gcatgaacac ggtcatgctg cagctggagg
     1201 aggtggagcg ggagcgggac caggccttcc actcccgaga tgaagctcag acacagtact
     1261 cgcagtgctt aatcgaaaag gacaagtaca ggaagcagat ccgcgagctg gaggagaaga
     1321 acgatgagat gaggatcgag atggtgcggc gggaggcctg catcgtcaac ctggagagca
     1381 agctgcggcg cctctccaag gacagcaaca acctggacca gagtctgccc aggaacctgc
     1441 cagtaaccat catctctcag gactttgggg atgccagccc caggaccaat ggtcaagaag
     1501 ctgacgattc ttccacctcg gaggagtcac ctgaagacag caagtacttc ctgccctacc
     1561 atccgcccca gcgcaggatg aacctgaagg gcatccagct gcagagagcc aaatccccca
     1621 tcagcctgaa gcgaacatca gattttcaag ccaaggggca cgaggaagaa ggcacggatg
     1681 ccagccctag ctcctgcgga tctctgccca tcaccaactc cttcaccaag atgcagcccc
     1741 cccggagccg cagcagcatc atgtcaatca ccgccgagcc cccgggaaac gactccatcg
     1801 tcagacgcta caaggaggac gcgccccatc gcagcacagt cgaagaagac aatgacagcg
     1861 gcgggtttga cgccttagat ctggatgatg acagtcacga acgctactcc ttcggaccct
     1921 cctccatcca ctcctcctcc tcctcccacc aatccgaggg cctggatgcc tacgacctgg
     1981 agcaggtcaa cctcatgttc aggaagttct ctctggaaag acccttccgg ccttcggtca
     2041 cctctgtggg gcacgtgcgg ggcccagggc cctcggtgca gcacacgacg ctgaatggcg
     2101 acagcctcac ctcccagctc accctgctgg ggggcaacgc gcgagggagc ttcgtgcact
     2161 cggtcaagcc tggctctctg gccgagaaag ccggcctccg tgagggccac cagctgctgc
     2221 tgctagaagg ctgcatccga ggcgagaggc agagtgtccc gttggacaca tgcaccaaag
     2281 aggaagccca ctggactatc cagaggtgca gcggccccgt cacgctgcac tacaaggtca
     2341 accacgaagg gtaccggaag ctggtgaagg acatggagga cggcctgatc acatcggggg
     2401 actcgttcta catccggctg aacctgaaca tctccagcca gctggacgcc tgcaccatgt
     2461 ccctgaagtg tgacgatgtt gtgcacgtcc gtgacaccat gtaccaggac aggcacgagt
     2521 ggctgtgcgc gcgggtcgac cctttcacag accatgacct ggatatgggc accataccca
     2581 gctacagccg agcccagcag ctcctcctgg tgaaactgca gcgcctgatg caccgaggca
     2641 gccgggagga ggtagacggc acccaccaca ccctgcgggc actccggaac accctgcagc
     2701 cagaagaagc gctttcaaca agcgaccccc gggtcagccc ccgtctctcg cgagcaagct
     2761 tcctttttgg ccagctcctt cagttcgtca gcaggtccga gaacaagtat aagcggatga
     2821 acagcaacga gcgggtccgc atcatctcgg ggagtccgct agggagcctg gcccggtcct
     2881 cgctggacgc caccaagctc ttgactgaga agcaggaaga gctggaccct gagagcgagc
     2941 tgggcaagaa cctcagcctc atcccctaca gcctggtacg cgccttctac tgcgagcgcc
     3001 gccggcccgt gctcttcaca cccaccgtgc tggccaagac gctggtgcag aggctgctca
     3061 actcgggagg tgccatggag ttcaccatct gcaagtcaga tatcgtcaca agagatgagt
     3121 tcctcagaag gcagaagacg gagaccatca tctactcccg agagaagaac cccaacgcgt
     3181 tcgaatgcat cgcccctgcc aacattgaag ctgtggccgc caagaacaag cactgcctgc
     3241 tggaggctgg gatcggctgc acaagagact tgatcaagtc caacatctac cccatcgtgc
     3301 tcttcatccg ggtgtgtgag aagaacatca agaggttcag aaagctgctg ccccgacctg
     3361 agacggagga ggagttcctg cgcgtgtgcc ggctgaagga gaaggagctg gaggccctgc
     3421 cgtgcctgta cgccacggtg gaacctgaca tgtggggcag cgtagaggag ctgctccgcg
     3481 ttgtcaagga caagatcggc gaggagcagc gcaagaccat ctgggtggac gaggaccagc
     3541 tgggatccgg tgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatccgg
     3601 gccccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3661 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3721 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3781 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3841 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3901 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3961 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4021 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4081 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4141 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4201 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4261 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4321 agtgaatcta gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4381 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4441 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4501 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4561 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4621 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4681 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4741 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4801 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4861 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4921 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     4981 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5041 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5101 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5161 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5221 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5281 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5341 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5401 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5461 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5521 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5581 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5641 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5701 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5761 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5821 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5881 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5941 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6001 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6061 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6121 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6181 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6241 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6301 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6361 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6721 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6781 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6901 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6961 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7021 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7081 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7141 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7201 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7261 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7321 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7381 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7561 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8101 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8161 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8221 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8281 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8341 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8401 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8461 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     8521 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8581 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8641 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8701 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     8761 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8821 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8881 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8941 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9001 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9061 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9121 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9181 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9241 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9301 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9361 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccctg
     9421 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9481 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9541 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9601 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9661 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9721 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9781 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9841 gctgtgcctt ggaatgctag ttggagtaat gaatctctgg aacagatttg gaatcacacg
     9901 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9961 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    10021 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    10081 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    10141 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    10201 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    10261 cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
    10321 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    10381 ttacaaaaac aaattacaaa aattcaaaat tttatcgacg cgtaggaaca gagaaacagg
    10441 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
    10501 aacagttgga acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc
    10561 cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga
    10621 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
    10681 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctctata
    10741 taagcagagc t
//
