LOCUS       sequence-323586-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of K41Q CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 K41Q-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3544..3597
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3598..3600,3601..3603,3604..4317)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3598..3600,3601..3603,3604..4314)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3643..3664)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3904..3923)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4251..4272
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4331..4919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4384..4404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4802..4813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5006..5239
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5311..5432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5348..5367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5402..5421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5472..5607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5627..5646)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5628..5646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5656..5673)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5656..5672)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5665..5687)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5814..6269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5901..5920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6111..6132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6295..6399
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6400..6468,6469..7260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6618..6637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7431..8019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7920..7939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8173..8190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8307..8328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8343..8348,8349..8366,8367..8373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8381..8397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8386..8408
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8405..8421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8405..8421
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8440..8460
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8442..8460
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8488..8714
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8715..8895
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8942..9067
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9559..9792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9977..10021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10288..10405
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     join(10740..10751,1..13)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacttccata gaaggatctc
       61 gacgccgcca ccatgccagg aggagggcca gagatggatg actacatgga gacgctgaag
      121 gatgaagagg acgccttgtg ggagaatgtg gagtgtaacc ggcacatgct cagccgctat
      181 atcaaccctg cccagctcac gccctacctg cgtcagtgta aggtcattga tgagcaggat
      241 gaagatgaag tgcttaatgc ccctatgctg ccatccaaga tcaaccgagc aggccggctg
      301 ttggacattc tacataccaa ggggcaaagg ggctatgtgg tcttcttgga gagcctagaa
      361 ttttattacc cagaactgta caaactggtg actgggaaag agcccactcg gagattctcc
      421 accattgtgg tggaggaagg ccacgagggc ctcacgcact tcctgatgaa cgaggtcatc
      481 aagctgcagc agcagatgaa ggccaaggac ctgcaacgct gcgagctgct ggccaggttg
      541 cggcagctgg aggatgagaa gaagcagatg acgctgacgc gcgtggagct gctaaccttc
      601 caggagcggt actacaagat gaaggaagag cgggacagct acaatgacga gctggtcaag
      661 gtgaaggacg acaactacaa cttagccatg cgctacgcac agctcagtga ggagaagaac
      721 atggcggtca tgaggagccg agacctccaa ctcgagatcg atcagctaaa gcaccggttg
      781 aataagatgg aggaggaatg taagctggag agaaatcagt ctctaaaact gaagaatgac
      841 attgaaaatc ggcccaagaa ggagcaggtt ctggaactgg agcgggagaa tgaaatgctg
      901 aagaccaaaa accaggagct gcagtccatc atccaggccg ggaagcgcag cctgccagac
      961 tcagacaagg ccatcctgga catcttggaa cacgaccgca aggaggccct ggaggacagg
     1021 caggagctgg tcaacaggat ctacaacctg caggaggagg cccgccaggc agaggagctg
     1081 cgagacaagt acctggagga gaaggaggac ctggagctca agtgctcgac cctgggaaag
     1141 gactgtgaaa tgtacaagca ccgcatgaac acggtcatgc tgcagctgga ggaggtggag
     1201 cgggagcggg accaggcctt ccactcccga gatgaagctc agacacagta ctcgcagtgc
     1261 ttaatcgaaa aggacaagta caggaagcag atccgcgagc tggaggagaa gaacgatgag
     1321 atgaggatcg agatggtgcg gcgggaggcc tgcatcgtca acctggagag caagctgcgg
     1381 cgcctctcca aggacagcaa caacctggac cagagtctgc ccaggaacct gccagtaacc
     1441 atcatctctc aggactttgg ggatgccagc cccaggacca atggtcaaga agctgacgat
     1501 tcttccacct cggaggagtc acctgaagac agcaagtact tcctgcccta ccatccgccc
     1561 cagcgcagga tgaacctgaa gggcatccag ctgcagagag ccaaatcccc catcagcctg
     1621 aagcgaacat cagattttca agccaagggg cacgaggaag aaggcacgga tgccagccct
     1681 agctcctgcg gatctctgcc catcaccaac tccttcacca agatgcagcc cccccggagc
     1741 cgcagcagca tcatgtcaat caccgccgag cccccgggaa acgactccat cgtcagacgc
     1801 tacaaggagg acgcgcccca tcgcagcaca gtcgaagaag acaatgacag cggcgggttt
     1861 gacgccttag atctggatga tgacagtcac gaacgctact ccttcggacc ctcctccatc
     1921 cactcctcct cctcctccca ccaatccgag ggcctggatg cctacgacct ggagcaggtc
     1981 aacctcatgt tcaggaagtt ctctctggaa agacccttcc ggccttcggt cacctctgtg
     2041 gggcacgtgc ggggcccagg gccctcggtg cagcacacga cgctgaatgg cgacagcctc
     2101 acctcccagc tcaccctgct ggggggcaac gcgcgaggga gcttcgtgca ctcggtcaag
     2161 cctggctctc tggccgagaa agccggcctc cgtgagggcc accagctgct gctgctagaa
     2221 ggctgcatcc gaggcgagag gcagagtgtc ccgttggaca catgcaccaa agaggaagcc
     2281 cactggacca tccagaggtg cagcggcccc gtcacgctgc actacaaggt caaccacgaa
     2341 gggtaccgga agctggtgaa ggacatggag gacggcctga tcacatcggg ggactcgttc
     2401 tacatccggc tgaacctgaa catctccagc cagctggacg cctgcaccat gtccctgaag
     2461 tgtgacgatg ttgtgcacgt ccgtgacacc atgtaccagg acaggcacga gtggctgtgc
     2521 gcgcgggtcg accctttcac agaccatgac ctggatatgg gcaccatacc cagctacagc
     2581 cgagcccagc agctcctcct ggtgaaactg cagcgcctga tgcaccgagg cagccgggag
     2641 gaggtagacg gcacccacca caccctgcgg gcactccgga acaccctgca gccagaagaa
     2701 gcgctttcaa caagcgaccc ccgggtcagc ccccgtctct cgcgagcaag cttccttttt
     2761 ggccagctcc ttcagttcgt cagcaggtcc gagaacaagt ataagcggat gaacagcaac
     2821 gagcgggtcc gcatcatctc ggggagtccg ctagggagcc tggcccggtc ctcgctggac
     2881 gccaccaagc tcttgactga gaagcaggaa gagctggacc ctgagagcga gctgggcaag
     2941 aacctcagcc tcatccccta cagcctggta cgcgccttct actgcgagcg ccgccggccc
     3001 gtgctcttca cacccaccgt gctggccaag acgctggtgc agaggctgct caactcggga
     3061 ggtgccatgg agttcaccat ctgcaagtca gatatcgtca caagagatga gttcctcaga
     3121 aggcagaaga cggagaccat catctactcc cgagagaaga accccaacgc gttcgaatgc
     3181 atcgcccctg ccaacattga agctgtggcc gccaagaaca agcactgcct gctggaggct
     3241 gggatcggct gcacaagaga cttgatcaag tccaacatct accccatcgt gctcttcatc
     3301 cgggtgtgtg agaagaacat caagaggttc agaaagctgc tgccccgacc tgagacggag
     3361 gaggagttcc tgcgcgtgtg ccggctgaag gagaaggagc tggaggccct gccgtgcctg
     3421 tacgccacgg tggaacctga catgtggggc agcgtagagg agctgctccg cgttgtcaag
     3481 gacaagatcg gcgaggagca gcgcaagacc atctgggtgg acgaggacca gctgggatcc
     3541 ggtgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc gggccccatg
     3601 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3661 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3721 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3781 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     3841 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3901 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3961 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4021 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4081 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4141 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4201 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4261 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgaatc
     4321 tagagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4741 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4921 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     4981 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5041 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5101 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5161 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5221 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     5281 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     5341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     5461 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5521 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5581 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     5641 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5701 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5761 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     5821 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5881 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5941 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6001 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6061 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6121 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6181 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6241 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6301 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6361 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6421 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6481 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6541 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6601 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6661 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6721 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6781 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6841 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6901 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6961 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7021 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7081 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7141 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7201 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7261 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7321 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7381 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7441 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7501 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7561 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7621 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7681 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7741 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7801 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7861 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7921 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7981 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8041 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8101 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8161 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8221 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8281 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8341 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8401 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     8461 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     8521 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     8581 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     8641 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     8701 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8761 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8821 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8881 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8941 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     9001 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     9061 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     9121 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     9181 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     9241 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     9301 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     9361 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccc tgatcttcag
     9421 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9481 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9541 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9601 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9661 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9721 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9781 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9841 ttggaatgct agttggagta atgaatctct ggaacagatt tggaatcaca cgacctggat
     9901 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9961 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10021 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10081 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10141 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    10201 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    10261 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
    10321 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    10381 acaaattaca aaaattcaaa attttatcga cgcgtaggaa cagagaaaca ggagaatatg
    10441 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagttg
    10501 gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
    10561 ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc
    10621 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
    10681 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcta tataagcaga
    10741 gctcgtttag t
//
