LOCUS       sequence-323588-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of K69L CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 K69L-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3556..3609
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3610..3612,3613..3615,3616..4329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3610..3612,3613..3615,3616..4326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3655..3676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3916..3935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4263..4284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4343..4931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4396..4416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4814..4825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5018..5251
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5323..5444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5360..5379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5414..5433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5484..5619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5639..5658)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5640..5658)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5668..5685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5668..5684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5677..5699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5826..6281
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5913..5932)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6123..6144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6307..6411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6412..6480,6481..7272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6630..6649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7443..8031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7932..7951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8185..8202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8319..8340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8355..8360,8361..8378,8379..8385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8393..8409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8398..8420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8417..8433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8417..8433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8452..8472
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8454..8472
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8500..8726
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8727..8907
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8954..9079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9571..9804
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9989..10033
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10300..10417
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacttcca
       61 tagaaggatc tcgacgccgc caccatgcca ggaggagggc cagagatgga tgactacatg
      121 gagacgctga aggatgaaga ggacgccttg tgggagaatg tggagtgtaa ccggcacatg
      181 ctcagccgct atatcaaccc tgccaagctc acgccctacc tgcgtcagtg taaggtcatt
      241 gatgagcagg atgaagatga agtgcttaat gcccctatgc tgccatccct gatcaaccga
      301 gcaggccggc tgttggacat tctacatacc aaggggcaaa ggggctatgt ggtcttcttg
      361 gagagcctag aattttatta cccagaactg tacaaactgg tgactgggaa agagcccact
      421 cggagattct ccaccattgt ggtggaggaa ggccacgagg gcctcacgca cttcctgatg
      481 aacgaggtca tcaagctgca gcagcagatg aaggccaagg acctgcaacg ctgcgagctg
      541 ctggccaggt tgcggcagct ggaggatgag aagaagcaga tgacgctgac gcgcgtggag
      601 ctgctaacct tccaggagcg gtactacaag atgaaggaag agcgggacag ctacaatgac
      661 gagctggtca aggtgaagga cgacaactac aacttagcca tgcgctacgc acagctcagt
      721 gaggagaaga acatggcggt catgaggagc cgagacctcc aactcgagat cgatcagcta
      781 aagcaccggt tgaataagat ggaggaggaa tgtaagctgg agagaaatca gtctctaaaa
      841 ctgaagaatg acattgaaaa tcggcccaag aaggagcagg ttctggaact ggagcgggag
      901 aatgaaatgc tgaagaccaa aaaccaggag ctgcagtcca tcatccaggc cgggaagcgc
      961 agcctgccag actcagacaa ggccatcctg gacatcttgg aacacgaccg caaggaggcc
     1021 ctggaggaca ggcaggagct ggtcaacagg atctacaacc tgcaggagga ggcccgccag
     1081 gcagaggagc tgcgagacaa gtacctggag gagaaggagg acctggagct caagtgctcg
     1141 accctgggaa aggactgtga aatgtacaag caccgcatga acacggtcat gctgcagctg
     1201 gaggaggtgg agcgggagcg ggaccaggcc ttccactccc gagatgaagc tcagacacag
     1261 tactcgcagt gcttaatcga aaaggacaag tacaggaagc agatccgcga gctggaggag
     1321 aagaacgatg agatgaggat cgagatggtg cggcgggagg cctgcatcgt caacctggag
     1381 agcaagctgc ggcgcctctc caaggacagc aacaacctgg accagagtct gcccaggaac
     1441 ctgccagtaa ccatcatctc tcaggacttt ggggatgcca gccccaggac caatggtcaa
     1501 gaagctgacg attcttccac ctcggaggag tcacctgaag acagcaagta cttcctgccc
     1561 taccatccgc cccagcgcag gatgaacctg aagggcatcc agctgcagag agccaaatcc
     1621 cccatcagcc tgaagcgaac atcagatttt caagccaagg ggcacgagga agaaggcacg
     1681 gatgccagcc ctagctcctg cggatctctg cccatcacca actccttcac caagatgcag
     1741 cccccccgga gccgcagcag catcatgtca atcaccgccg agcccccggg aaacgactcc
     1801 atcgtcagac gctacaagga ggacgcgccc catcgcagca cagtcgaaga agacaatgac
     1861 agcggcgggt ttgacgcctt agatctggat gatgacagtc acgaacgcta ctccttcgga
     1921 ccctcctcca tccactcctc ctcctcctcc caccaatccg agggcctgga tgcctacgac
     1981 ctggagcagg tcaacctcat gttcaggaag ttctctctgg aaagaccctt ccggccttcg
     2041 gtcacctctg tggggcacgt gcggggccca gggccctcgg tgcagcacac gacgctgaat
     2101 ggcgacagcc tcacctccca gctcaccctg ctggggggca acgcgcgagg gagcttcgtg
     2161 cactcggtca agcctggctc tctggccgag aaagccggcc tccgtgaggg ccaccagctg
     2221 ctgctgctag aaggctgcat ccgaggcgag aggcagagtg tcccgttgga cacatgcacc
     2281 aaagaggaag cccactggac catccagagg tgcagcggcc ccgtcacgct gcactacaag
     2341 gtcaaccacg aagggtaccg gaagctggtg aaggacatgg aggacggcct gatcacatcg
     2401 ggggactcgt tctacatccg gctgaacctg aacatctcca gccagctgga cgcctgcacc
     2461 atgtccctga agtgtgacga tgttgtgcac gtccgtgaca ccatgtacca ggacaggcac
     2521 gagtggctgt gcgcgcgggt cgaccctttc acagaccatg acctggatat gggcaccata
     2581 cccagctaca gccgagccca gcagctcctc ctggtgaaac tgcagcgcct gatgcaccga
     2641 ggcagccggg aggaggtaga cggcacccac cacaccctgc gggcactccg gaacaccctg
     2701 cagccagaag aagcgctttc aacaagcgac ccccgggtca gcccccgtct ctcgcgagca
     2761 agcttccttt ttggccagct ccttcagttc gtcagcaggt ccgagaacaa gtataagcgg
     2821 atgaacagca acgagcgggt ccgcatcatc tcggggagtc cgctagggag cctggcccgg
     2881 tcctcgctgg acgccaccaa gctcttgact gagaagcagg aagagctgga ccctgagagc
     2941 gagctgggca agaacctcag cctcatcccc tacagcctgg tacgcgcctt ctactgcgag
     3001 cgccgccggc ccgtgctctt cacacccacc gtgctggcca agacgctggt gcagaggctg
     3061 ctcaactcgg gaggtgccat ggagttcacc atctgcaagt cagatatcgt cacaagagat
     3121 gagttcctca gaaggcagaa gacggagacc atcatctact cccgagagaa gaaccccaac
     3181 gcgttcgaat gcatcgcccc tgccaacatt gaagctgtgg ccgccaagaa caagcactgc
     3241 ctgctggagg ctgggatcgg ctgcacaaga gacttgatca agtccaacat ctaccccatc
     3301 gtgctcttca tccgggtgtg tgagaagaac atcaagaggt tcagaaagct gctgccccga
     3361 cctgagacgg aggaggagtt cctgcgcgtg tgccggctga aggagaagga gctggaggcc
     3421 ctgccgtgcc tgtacgccac ggtggaacct gacatgtggg gcagcgtaga ggagctgctc
     3481 cgcgttgtca aggacaagat cggcgaggag cagcgcaaga ccatctgggt ggacgaggac
     3541 cagctgggat ccggtgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     3601 ccgggcccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3781 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4021 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4081 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4201 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4321 tacaagtgaa tctagagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4921 cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta caaggcagct
     4981 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5041 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5221 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     5281 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5341 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5401 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5461 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5521 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5581 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5641 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     5701 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5761 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5821 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5881 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5941 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6001 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6061 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6121 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6181 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6241 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6301 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6361 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6421 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6481 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6541 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6601 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6661 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6721 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6781 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6841 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6901 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6961 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7021 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7081 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7141 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7201 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7261 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7321 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7381 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7441 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7501 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7561 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7621 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7681 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7801 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7861 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8041 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8101 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8161 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8221 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8281 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8341 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8401 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     8461 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     8521 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     8581 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     8641 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     8701 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     8761 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8821 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8881 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     8941 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9001 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9061 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9121 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9181 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9241 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9301 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9361 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9421 cctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     9481 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     9541 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     9601 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     9661 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9721 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     9781 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     9841 cactgctgtg ccttggaatg ctagttggag taatgaatct ctggaacaga tttggaatca
     9901 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     9961 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    10021 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    10081 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    10141 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    10201 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
    10261 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
    10321 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    10381 agaattacaa aaacaaatta caaaaattca aaattttatc gacgcgtagg aacagagaaa
    10441 caggagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
    10501 caagaacagt tggaacagca gaatatgggc caaacaggat atctgtggta agcagttcct
    10561 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
    10621 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
    10681 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
    10741 tatataagca g
//
