LOCUS       sequence-323589-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of R35Q CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 R35Q-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    332..449
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(716..760)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    945..1178
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1670..1795
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1842..2022
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2023..2249)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2277..2297)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2277..2295)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2316..2332)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2316..2332)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2329..2351)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2340..2356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2364..2370,2371..2388,2389..2394))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2409..2430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2547..2564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2718..3306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2798..2817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3477..4268,4269..4337))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4100..4119
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4338..4442)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4468..4923)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4605..4626)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4817..4836
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5050..5072
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5064..5081
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5065..5081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5091..5110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5091..5109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5130..5265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5305..5426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5316..5335)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5370..5389
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5498..5731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5818..6406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5924..5935
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6333..6353
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6420..7133,7134..7136,7137..7139))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6423..7133,7134..7136,7137..7139))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6465..6486)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6814..6833
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7073..7094
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(7140..7193)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(10724..10748)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cttatataga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
       61 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
      121 gttctctaga aactgctgag ggcgggaccg catctgggga ccatctgttc ttggccctga
      181 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattctg ctgttccaac
      241 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
      301 ttctcctgtt tctctgttcc tacgcgtcga taaaattttg aatttttgta atttgttttt
      361 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
      421 accccccaat cccccctttt cttttaaaag ttaaccgata ccgtcgagat ccgttcacta
      481 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
      541 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
      601 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
      661 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
      721 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
      781 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc
      841 aggtcgtgtg attccaaatc tgttccagag attcattact ccaactagca ttccaaggca
      901 cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct
      961 ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga
     1021 gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta
     1081 taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc
     1141 ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc
     1201 tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta
     1261 ctttatattt atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga
     1321 agatcagggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     1381 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     1441 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     1501 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     1561 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     1621 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     1681 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     1741 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     1801 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     1861 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     1921 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     1981 agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag
     2041 gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag
     2101 acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca
     2161 tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca
     2221 agactacaag agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt
     2281 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2341 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2401 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2461 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2521 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2581 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2641 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2701 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2761 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2821 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2881 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2941 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3001 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3061 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3421 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3481 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3541 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3601 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3661 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3721 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3781 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3841 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3901 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3961 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4021 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4081 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4141 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4201 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4261 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4321 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4381 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4441 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     4501 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4561 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     4621 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     4681 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     4741 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     4801 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4861 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4921 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4981 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     5041 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     5101 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     5161 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5221 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5281 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     5341 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5401 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     5461 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     5521 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     5581 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     5641 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     5701 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     5761 agatctacag ctgccttgta agtcattggt cttaaaggta ccgagctcga attccaggcg
     5821 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     5881 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     5941 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccatggaa
     6001 aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     6061 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     6121 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     6181 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     6241 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     6301 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     6361 gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg actctagatt
     6421 cacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     6481 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     6541 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     6601 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     6661 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     6721 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     6781 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     6841 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     6901 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     6961 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     7021 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     7081 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     7141 gggcccggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctcaccggat
     7201 cccagctggt cctcgtccac ccagatggtc ttgcgctgct cctcgccgat cttgtccttg
     7261 acaacgcgga gcagctcctc tacgctgccc cacatgtcag gttccaccgt ggcgtacagg
     7321 cacggcaggg cctccagctc cttctccttc agccggcaca cgcgcaggaa ctcctcctcc
     7381 gtctcaggtc ggggcagcag ctttctgaac ctcttgatgt tcttctcaca cacccggatg
     7441 aagagcacga tggggtagat gttggacttg atcaagtctc ttgtgcagcc gatcccagcc
     7501 tccagcaggc agtgcttgtt cttggcggcc acagcttcaa tgttggcagg ggcgatgcat
     7561 tcgaacgcgt tggggttctt ctctcgggag tagatgatgg tctccgtctt ctgccttctg
     7621 aggaactcat ctcttgtgac gatatctgac ttgcagatgg tgaactccat ggcacctccc
     7681 gagttgagca gcctctgcac cagcgtcttg gccagcacgg tgggtgtgaa gagcacgggc
     7741 cggcggcgct cgcagtagaa ggcgcgtacc aggctgtagg ggatgaggct gaggttcttg
     7801 cccagctcgc tctcagggtc cagctcttcc tgcttctcag tcaagagctt ggtggcgtcc
     7861 agcgaggacc gggccaggct cactagcgga ctccccgaga tgatgcggac ccgctcgttg
     7921 ctgttcatcc gcttatactt gttctcggac ctgctgacga actgaaggag ctggccaaaa
     7981 aggaagcttg ctcgcgagag acgggggctg acccgggggt cgcttgttga aagcgcttct
     8041 tctggctgca gggtgttccg gagtgcccgc agggtgtggt gggtgccgtc tacctcctcc
     8101 cggctgcctc ggtgcatcag gcgctgcagt ttcaccagga ggagctgctg ggctcggctg
     8161 tagctgggta tggtgcccat atccaggtca tggtctgtga aagggtcgac ccgcgcgcac
     8221 agccactcgt gcctgtcctg gtacatggtg tcacggacgt gcacaacatc gtcacacttc
     8281 agggacatgg tgcaggcgtc cagctggctg gagatgttca ggttcagccg gatgtagaac
     8341 gagtcccccg atgtgatcag gccgtcctcc atgtccttca ccagcttccg gtacccttcg
     8401 tggttgacct tgtagtgcag cgtgacgggg ccgctgcacc tctggatggt ccagtgggct
     8461 tcctctttgg tgcatgtgtc caacgggaca ctctgcctct cgcctcggat gcagccttct
     8521 agcagcagca gctggtggcc ctcacggagg ccggctttct cggccagaga gccaggcttg
     8581 accgagtgca cgaagctccc tcgcgcgttg ccccccagca gggtgagctg ggaggtgagg
     8641 ctgtcgccat tcagcgtcgt gtgctgcacc gagggccctg ggccccgcac gtgccccaca
     8701 gaggtgaccg aaggccggaa gggtctttcc agagagaact tcctgaacat gaggttgacc
     8761 tgctccaggt cgtaggcatc caggccctcg gattggtggg aggaggagga ggagtggatg
     8821 gaggagggtc cgaaggagta gcgttcgtga ctgtcatcat ccagatctaa ggcgtcaaac
     8881 ccgccgctgt cattgtcttc ttcgactgtg ctgcgatggg gcgcgtcctc cttgtagcgt
     8941 ctgacgatgg agtcgtttcc cgggggctcg gcggtgattg acatgatgct gctgcggctc
     9001 cgggggggct gcatcttggt gaaggagttg gtgatgggca gagatccgca ggagctaggg
     9061 ctggcatccg tgccttcttc ctcgtgcccc ttggcttgaa aatctgatgt tcgcttcagg
     9121 ctgatggggg atttggctct ctgcagctgg atgcccttca ggttcatcct gcgctggggc
     9181 ggatggtagg gcaggaagta cttgctgtct tcaggtgact cctccgaggt ggaagaatcg
     9241 tcagcttctt gaccattggt cctggggctg gcatccccaa agtcctgaga gatgatggtt
     9301 actggcaggt tcctgggcag actctggtcc aggttgttgc tgtccttgga gaggcgccgc
     9361 agcttgctct ccaggttgac gatgcaggcc tcccgccgca ccatctcgat cctcatctca
     9421 tcgttcttct cctccagctc gcggatctgc ttcctgtact tgtccttttc gattaagcac
     9481 tgcgagtact gtgtctgagc ttcatctcgg gagtggaagg cctggtcccg ctcccgctcc
     9541 acctcctcca gctgcagcat gaccgtgttc atgcggtgct tgtacatttc acagtccttt
     9601 cccagggtcg agcacttgag ctccaggtcc tccttctcct ccaggtactt gtctcgcagc
     9661 tcctctgcct ggcgggcctc ctcctgcagg ttgtagatcc tgttgaccag ctcctgcctg
     9721 tcctccaggg cctccttgcg gtcgtgttcc aagatgtcca ggatggcctt gtctgagtct
     9781 ggcaggctgc gcttcccggc ctggatgatg gactgcagct cctggttttt ggtcttcagc
     9841 atttcattct cccgctccag ttccagaacc tgctccttct tgggccgatt ttcaatgtca
     9901 ttcttcagtt ttagagactg atttctctcc agcttacatt cctcctccat cttattcaac
     9961 cggtgcttta gctgatcgat ctcgagttgg aggtctcggc tcctcatgac cgccatgttc
    10021 ttctcctcac tgagctgtgc gtagcgcatg gctaagttgt agttgtcgtc cttcaccttg
    10081 accagctcgt cattgtagct gtcccgctct tccttcatct tgtagtaccg ctcctggaag
    10141 gttagcagct ccacgcgcgt cagcgtcatc tgcttcttct catcctccag ctgccgcaac
    10201 ctggccagca gctcgcagcg ttgcaggtcc ttggccttca tctgctgctg cagcttgatg
    10261 acctcgttca tcaggaagtg cgtgaggccc tcgtggcctt cctccaccac aatggtggag
    10321 aatctccgag tgggctcttt cccagtcacc agtttgtaca gttctgggta ataaaattct
    10381 aggctctcca agaagaccac atagcccctt tgccccttgg tatgtagaat gtccaacagc
    10441 cggcctgctc ggttgatctt ggatggcagc ataggggcat taagcacttc atcttcatcc
    10501 tgctcatcaa tgaccttaca ctgacgcagg tagggcgtga gcttggcagg gttgatatac
    10561 tggctgagca tgtgccggtt acactccaca ttctcccaca aggcgtcctc ttcatccttc
    10621 agcgtctcca tgtagtcatc catctctggc cctcctcctg gcatggtggc ggcgtcgaga
    10681 tccttctatg gaagtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
    10741 aacgagctct g
//
