LOCUS       sequence-323594-       10538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CARD domain variant with a deletion of exon 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 delta e3-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3327..3380
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3381..3383,3384..3386,3387..4100)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3381..3383,3384..3386,3387..4097)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3426..3447)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3687..3706)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4034..4055
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4114..4702
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4167..4187)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4585..4596)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4789..5022
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5094..5215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5131..5150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5185..5204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5255..5390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5410..5429)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5411..5429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5439..5456)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5439..5455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5448..5470)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5597..6052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5684..5703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5894..5915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6078..6182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6183..6251,6252..7043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6401..6420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7214..7802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7703..7722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7956..7973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8090..8111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8126..8131,8132..8149,8150..8156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8164..8180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8169..8191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8188..8204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8188..8204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8223..8243
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8225..8243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8271..8497
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8498..8678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8725..8850
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9342..9575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9760..9804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10071..10188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     join(10523..10538,1..9)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgtcagatcg cctggagacg ccatccacgc tgttttgact tccatagaag gatctcgacg
       61 ccgccaccat gccaggccgg ctgttggaca ttctacatac caaggggcaa aggggctatg
      121 tggtcttctt ggagagccta gaattttatt acccagaact gtacaaactg gtgactggga
      181 aagagcccac tcggagattc tccaccattg tggtggagga aggccacgag ggcctcacgc
      241 acttcctgat gaacgaggtc atcaagctgc agcagcagat gaaggccaag gacctgcaac
      301 gctgcgagct gctggccagg ttgcggcagc tggaggatga gaagaagcag atgacgctga
      361 cgcgcgtgga gctgctaacc ttccaggagc ggtactacaa gatgaaggaa gagcgggaca
      421 gctacaatga cgagctggtc aaggtgaagg acgacaacta caacttagcc atgcgctacg
      481 cacagctcag tgaggagaag aacatggcgg tcatgaggag ccgagacctc caactcgaga
      541 tcgatcagct aaagcaccgg ttgaataaga tggaggagga atgtaagctg gagagaaatc
      601 agtctctaaa actgaagaat gacattgaaa atcggcccaa gaaggagcag gttctggaac
      661 tggagcggga gaatgaaatg ctgaagacca aaaaccagga gctgcagtcc atcatccagg
      721 ccgggaagcg cagcctgcca gactcagaca aggccatcct ggacatcttg gaacacgacc
      781 gcaaggaggc cctggaggac aggcaggagc tggtcaacag gatctacaac ctgcaggagg
      841 aggcccgcca ggcagaggag ctgcgagaca agtacctgga ggagaaggag gacctggagc
      901 tcaagtgctc gaccctggga aaggactgtg aaatgtacaa gcaccgcatg aacacggtca
      961 tgctgcagct ggaggaggtg gagcgggagc gggaccaggc cttccactcc cgagatgaag
     1021 ctcagacaca gtactcgcag tgcttaatcg aaaaggacaa gtacaggaag cagatccgcg
     1081 agctggagga gaagaacgat gagatgagga tcgagatggt gcggcgggag gcctgcatcg
     1141 tcaacctgga gagcaagctg cggcgcctct ccaaggacag caacaacctg gaccagagtc
     1201 tgcccaggaa cctgccagta accatcatct ctcaggactt tggggatgcc agccccagga
     1261 ccaatggtca agaagctgac gattcttcca cctcggagga gtcacctgaa gacagcaagt
     1321 acttcctgcc ctaccatccg ccccagcgca ggatgaacct gaagggcatc cagctgcaga
     1381 gagccaaatc ccccatcagc ctgaagcgaa catcagattt tcaagccaag gggcacgagg
     1441 aagaaggcac ggatgccagc cctagctcct gcggatctct gcccatcacc aactccttca
     1501 ccaagatgca gcccccccgg agccgcagca gcatcatgtc aatcaccgcc gagcccccgg
     1561 gaaacgactc catcgtcaga cgctacaagg aggacgcgcc ccatcgcagc acagtcgaag
     1621 aagacaatga cagcggcggg tttgacgcct tagatctgga tgatgacagt cacgaacgct
     1681 actccttcgg accctcctcc atccactcct cctcctcctc ccaccaatcc gagggcctgg
     1741 atgcctacga cctggagcag gtcaacctca tgttcaggaa gttctctctg gaaagaccct
     1801 tccggccttc ggtcacctct gtggggcacg tgcggggccc agggccctcg gtgcagcaca
     1861 cgacgctgaa tggcgacagc ctcacctccc agctcaccct gctggggggc aacgcgcgag
     1921 ggagcttcgt gcactcggtc aagcctggct ctctggccga gaaagccggc ctccgtgagg
     1981 gccaccagct gctgctgcta gaaggctgca tccgaggcga gaggcagagt gtcccgttgg
     2041 acacatgcac caaagaggaa gcccactgga ccatccagag gtgcagcggc cccgtcacgc
     2101 tgcactacaa ggtcaaccac gaagggtacc ggaagctggt gaaggacatg gaggacggcc
     2161 tgatcacatc gggggactcg ttctacatcc ggctgaacct gaacatctcc agccagctgg
     2221 acgcctgcac catgtccctg aagtgtgacg atgttgtgca cgtccgtgac accatgtacc
     2281 aggacaggca cgagtggctg tgcgcgcggg tcgacccttt cacagaccat gacctggata
     2341 tgggcaccat acccagctac agccgagccc agcagctcct cctggtgaaa ctgcagcgcc
     2401 tgatgcaccg aggcagccgg gaggaggtag acggcaccca ccacaccctg cgggcactcc
     2461 ggaacaccct gcagccagaa gaagcgcttt caacaagcga cccccgggtc agcccccgtc
     2521 tctcgcgagc aagcttcctt tttggccagc tccttcagtt cgtcagcagg tccgagaaca
     2581 agtataagcg gatgaacagc aacgagcggg tccgcatcat ctcggggagt ccgctaggga
     2641 gcctggcccg gtcctcgctg gacgccacca agctcttgac tgagaagcag gaagagctgg
     2701 accctgagag cgagctgggc aagaacctca gcctcatccc ctacagcctg gtacgcgcct
     2761 tctactgcga gcgccgccgg cccgtgctct tcacacccac cgtgctggcc aagacgctgg
     2821 tgcagaggct gctcaactcg ggaggtgcca tggagttcac catctgcaag tcagatatcg
     2881 tcacaagaga tgagttcctc agaaggcaga agacggagac catcatctac tcccgagaga
     2941 agaaccccaa cgcgttcgaa tgcatcgccc ctgccaacat tgaagctgtg gccgccaaga
     3001 acaagcactg cctgctggag gctgggatcg gctgcacaag agacttgatc aagtccaaca
     3061 tctaccccat cgtgctcttc atccgggtgt gtgagaagaa catcaagagg ttcagaaagc
     3121 tgctgccccg acctgagacg gaggaggagt tcctgcgcgt gtgccggctg aaggagaagg
     3181 agctggaggc cctgccgtgc ctgtacgcca cggtggaacc tgacatgtgg ggcagcgtag
     3241 aggagctgct ccgcgttgtc aaggacaaga tcggcgagga gcagcgcaag accatctggg
     3301 tggacgagga ccagctggga tccggtgagg gcagaggaag tcttctaaca tgcggtgacg
     3361 tggaggagaa tccgggcccc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3421 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3481 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3541 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     3601 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3661 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3721 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3781 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3841 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     3901 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3961 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     4021 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4081 tggacgagct gtacaagtga atctagagtc gacaatcaac ctctggatta caaaatttgt
     4141 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4201 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4261 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4321 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4381 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4441 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4501 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4561 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4621 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4681 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt
     4741 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4801 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4861 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4921 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4981 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5041 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5221 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5281 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5341 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5401 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5461 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5521 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5581 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6001 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6061 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6421 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6721 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7321 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7561 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7621 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7681 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7741 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7801 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7861 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7921 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7981 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8041 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8101 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8161 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8221 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8281 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     8341 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     8401 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     8461 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8641 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8701 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8761 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8821 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8881 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8941 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9001 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9061 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9121 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9181 cagcaagcgg ccctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9241 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9301 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9361 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9421 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9481 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9541 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9601 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaatgaatc tctggaacag
     9661 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9721 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9781 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9841 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9901 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9961 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    10021 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
    10081 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    10141 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat cgacgcgtag
    10201 gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc
    10261 cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt
    10321 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
    10381 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
    10441 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
    10501 tccccgagct ctatataagc agagctcgtt tagtgaac
//
