LOCUS       sequence-323596-       10400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CARD domain variant with a deletion of exons 3 and 4. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 delta e3-4-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    845..1078
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1263..1307
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1574..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2026..2050
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5230..5283
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5284..5286,5287..5289,5290..6003)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5284..5286,5287..5289,5290..6000)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5329..5350)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5590..5609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5937..5958
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6017..6605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6070..6090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6488..6499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6692..6925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6997..7118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7034..7053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7088..7107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7158..7293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7313..7332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7314..7332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7342..7359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7342..7358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7351..7373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7500..7955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7587..7606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7797..7818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7981..8085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8086..8154,8155..8946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8304..8323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9117..9705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9606..9625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9859..9876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9993..10014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10029..10034,10035..10052,10053..10059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10067..10083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10072..10094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10091..10107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10091..10107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10126..10146
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10128..10146
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10174..10400
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccctgat cttcagacct ggaggaggag
      721 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
      781 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
      841 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
      901 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
      961 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1021 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1081 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1141 ggagtaatga atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1201 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1261 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1321 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1381 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1441 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1501 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     1561 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1621 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     1681 ttcaaaattt tatcgacgcg taggaacaga gaaacaggag aatatgggcc aaacaggata
     1741 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagttggaac agcagaatat
     1801 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     1861 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
     1921 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     1981 cgcttctgtt cgcgcgcttc tgctccccga gctctatata agcagagctc gtttagtgaa
     2041 ccgtcagatc gcctggagac gccatccacg ctgttttgac ttccatagaa ggatctcgac
     2101 gccgccacca tgccagtgga ggaaggccac gagggcctca cgcacttcct gatgaacgag
     2161 gtcatcaagc tgcagcagca gatgaaggcc aaggacctgc aacgctgcga gctgctggcc
     2221 aggttgcggc agctggagga tgagaagaag cagatgacgc tgacgcgcgt ggagctgcta
     2281 accttccagg agcggtacta caagatgaag gaagagcggg acagctacaa tgacgagctg
     2341 gtcaaggtga aggacgacaa ctacaactta gccatgcgct acgcacagct cagtgaggag
     2401 aagaacatgg cggtcatgag gagccgagac ctccaactcg agatcgatca gctaaagcac
     2461 cggttgaata agatggagga ggaatgtaag ctggagagaa atcagtctct aaaactgaag
     2521 aatgacattg aaaatcggcc caagaaggag caggttctgg aactggagcg ggagaatgaa
     2581 atgctgaaga ccaaaaacca ggagctgcag tccatcatcc aggccgggaa gcgcagcctg
     2641 ccagactcag acaaggccat cctggacatc ttggaacacg accgcaagga ggccctggag
     2701 gacaggcagg agctggtcaa caggatctac aacctgcagg aggaggcccg ccaggcagag
     2761 gagctgcgag acaagtacct ggaggagaag gaggacctgg agctcaagtg ctcgaccctg
     2821 ggaaaggact gtgaaatgta caagcaccgc atgaacacgg tcatgctgca gctggaggag
     2881 gtggagcggg agcgggacca ggccttccac tcccgagatg aagctcagac acagtactcg
     2941 cagtgcttaa tcgaaaagga caagtacagg aagcagatcc gcgagctgga ggagaagaac
     3001 gatgagatga ggatcgagat ggtgcggcgg gaggcctgca tcgtcaacct ggagagcaag
     3061 ctgcggcgcc tctccaagga cagcaacaac ctggaccaga gtctgcccag gaacctgcca
     3121 gtaaccatca tctctcagga ctttggggat gccagcccca ggaccaatgg tcaagaagct
     3181 gacgattctt ccacctcgga ggagtcacct gaagacagca agtacttcct gccctaccat
     3241 ccgccccagc gcaggatgaa cctgaagggc atccagctgc agagagccaa atcccccatc
     3301 agcctgaagc gaacatcaga ttttcaagcc aaggggcacg aggaagaagg cacggatgcc
     3361 agccctagct cctgcggatc tctgcccatc accaactcct tcaccaagat gcagcccccc
     3421 cggagccgca gcagcatcat gtcaatcacc gccgagcccc cgggaaacga ctccatcgtc
     3481 agacgctaca aggaggacgc gccccatcgc agcacagtcg aagaagacaa tgacagcggc
     3541 gggtttgacg ccttagatct ggatgatgac agtcacgaac gctactcctt cggaccctcc
     3601 tccatccact cctcctcctc ctcccaccaa tccgagggcc tggatgccta cgacctggag
     3661 caggtcaacc tcatgttcag gaagttctct ctggaaagac ccttccggcc ttcggtcacc
     3721 tctgtggggc acgtgcgggg cccagggccc tcggtgcagc acacgacgct gaatggcgac
     3781 agcctcacct cccagctcac cctgctgggg ggcaacgcgc gagggagctt cgtgcactcg
     3841 gtcaagcctg gctctctggc cgagaaagcc ggcctccgtg agggccacca gctgctgctg
     3901 ctagaaggct gcatccgagg cgagaggcag agtgtcccgt tggacacatg caccaaagag
     3961 gaagcccact ggaccatcca gaggtgcagc ggccccgtca cgctgcacta caaggtcaac
     4021 cacgaagggt accggaagct ggtgaaggac atggaggacg gcctgatcac atcgggggac
     4081 tcgttctaca tccggctgaa cctgaacatc tccagccagc tggacgcctg caccatgtcc
     4141 ctgaagtgtg acgatgttgt gcacgtccgt gacaccatgt accaggacag gcacgagtgg
     4201 ctgtgcgcgc gggtcgaccc tttcacagac catgacctgg atatgggcac catacccagc
     4261 tacagccgag cccagcagct cctcctggtg aaactgcagc gcctgatgca ccgaggcagc
     4321 cgggaggagg tagacggcac ccaccacacc ctgcgggcac tccggaacac cctgcagcca
     4381 gaagaagcgc tttcaacaag cgacccccgg gtcagccccc gtctctcgcg agcaagcttc
     4441 ctttttggcc agctccttca gttcgtcagc aggtccgaga acaagtataa gcggatgaac
     4501 agcaacgagc gggtccgcat catctcgggg agtccgctag ggagcctggc ccggtcctcg
     4561 ctggacgcca ccaagctctt gactgagaag caggaagagc tggaccctga gagcgagctg
     4621 ggcaagaacc tcagcctcat cccctacagc ctggtacgcg ccttctactg cgagcgccgc
     4681 cggcccgtgc tcttcacacc caccgtgctg gccaagacgc tggtgcagag gctgctcaac
     4741 tcgggaggtg ccatggagtt caccatctgc aagtcagata tcgtcacaag agatgagttc
     4801 ctcagaaggc agaagacgga gaccatcatc tactcccgag agaagaaccc caacgcgttc
     4861 gaatgcatcg cccctgccaa cattgaagct gtggccgcca agaacaagca ctgcctgctg
     4921 gaggctggga tcggctgcac aagagacttg atcaagtcca acatctaccc catcgtgctc
     4981 ttcatccggg tgtgtgagaa gaacatcaag aggttcagaa agctgctgcc ccgacctgag
     5041 acggaggagg agttcctgcg cgtgtgccgg ctgaaggaga aggagctgga ggccctgccg
     5101 tgcctgtacg ccacggtgga acctgacatg tggggcagcg tagaggagct gctccgcgtt
     5161 gtcaaggaca agatcggcga ggagcagcgc aagaccatct gggtggacga ggaccagctg
     5221 ggatccggtg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatccgggc
     5281 cccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5341 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5401 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5461 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5521 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5581 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5641 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5701 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5761 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5821 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5881 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     5941 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6001 tgaatctaga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6061 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6121 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6181 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6241 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6301 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6361 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     6421 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6481 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6541 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6601 cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     6661 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6721 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6781 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6841 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6901 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6961 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7081 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7141 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7201 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7261 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     7321 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7381 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7441 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7501 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7561 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7621 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7681 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7741 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7801 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7861 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7921 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     7981 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9241 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9781 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9841 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     9901 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9961 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    10021 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    10081 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
    10141 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
    10201 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
    10261 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
    10321 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
    10381 gcctagctcg atacataaac
//
