LOCUS       sequence-323599-        8768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LbCpf1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, 
            Huang X, Yang L, Chen J
  TITLE     Base editing with a Cpf1-cytidine deaminase fusion.
  JOURNAL   Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
  PUBMED    29553573
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102."
COMMENT     Sequence Label: pST1374-Lb-Cpf1-NLS
FEATURES             Location/Qualifiers
     source          1..8768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..4590
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4597..4617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4633..4674
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4684..4701
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4724..4741)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4730..4954
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5000..5428
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5087..5106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5297..5318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5437..5457)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5442..5771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5622..5757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5684..5703
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5819..5866
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5885..6283
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6441..6562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6478..6497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6532..6551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6611..6627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6611..6627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6624..6646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6635..6651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6659..6665,6666..6683,6684..6689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6704..6725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6842..6859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7013..7601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7093..7112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7772..8563,8564..8632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8395..8414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8633..8737)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgt caaagctgga gaaattcacc aactgttata gcctgtctaa gaccctgcgc
      961 ttcaaggcaa tcccagtggg caagacacaa gagaacattg acaacaaacg gctcctggtg
     1021 gaggatgaga agagggctga agattacaag ggcgttaaga agctgctgga taggtactat
     1081 ctgtcattca tcaacgatgt cctccacagt atcaagctga agaatctgaa caattacatt
     1141 tctctgttcc ggaagaagac acggaccgag aaggagaaca aagagctgga gaatctggag
     1201 atcaacctga ggaaagaaat agctaaggct ttcaaaggga acgagggtta caagtccctg
     1261 ttcaagaaag acattatcga gactattctg cctgagttcc tggacgataa agatgagatc
     1321 gccctcgtca attccttcaa tgggtttacc acagccttta ccggcttctt cgacaataga
     1381 gagaatatgt tctctgaaga ggccaaatcc actagcatcg cctttcgctg cataaacgag
     1441 aacctgacta ggtacatcag caatatggac atctttgaga aagtcgatgc catattcgac
     1501 aaacatgagg tgcaggagat taaggagaag atcctgaact cagattacga tgtcgaagat
     1561 ttcttcgagg gagagttctt caacttcgtg ctcacacaag agggcattga tgtgtacaat
     1621 gcaatcattg gagggttcgt gacagagagt ggcgagaaga taaagggcct gaacgagtat
     1681 atcaacctct acaaccagaa aaccaagcag aaactgccta agttcaagcc actgtacaaa
     1741 caagtgctct cagataggga aagcctgagc ttctacggtg aagggtatac atcagatgaa
     1801 gaagtgctcg aagtgttccg caacaccctc aataagaaca gtgaaatctt ctcttcaatc
     1861 aagaagctgg agaaactgtt caagaatttc gatgagtact cctctgccgg aatctttgtg
     1921 aagaatggcc ctgcaatatc cactattagc aaagacatct ttggcgagtg gaacgttatc
     1981 agggataagt ggaatgccga gtacgatgat attcatctca agaagaaagc cgtggttaca
     2041 gagaaatacg aggatgatag acgcaagagc tttaagaaga ttggtagctt ctctctcgaa
     2101 cagctgcagg agtacgccga cgctgacctg tcagtcgtgg agaaactcaa ggagatcata
     2161 atccagaagg tggatgaaat ctacaaagtg tatggaagct ctgagaaact cttcgatgca
     2221 gactttgttc tggagaagag tctgaagaag aacgacgcag tggttgctat catgaaggac
     2281 ctgctggatt ctgttaagtc tttcgagaat tacattaagg cattctttgg tgaagggaag
     2341 gagacaaata gggacgagag cttctatggc gactttgttc tggcctacga catcctcctc
     2401 aaggttgacc acatctatga cgctatacgg aattacgtta cccagaagcc ctatagcaaa
     2461 gacaagttca agctgtattt ccagaatcca cagtttatgg gtgggtggga taaagacaaa
     2521 gaaacagatt acagggccac tatcctgcgg tacggcagca aatactatct ggctatcatg
     2581 gataagaagt acgccaaatg cctccagaag atcgacaagg acgacgtgaa cggtaactac
     2641 gagaagatca attacaagct cctgccagga cctaacaaga tgctgcccaa ggtgttcttc
     2701 tccaagaaat ggatggccta ctataaccca agcgaggaca ttcagaagat atacaagaat
     2761 gggacattca agaagggcga tatgttcaac ctcaacgact gccacaagct gattgatttc
     2821 ttcaaggata gcatttctcg ctatcccaag tggtctaatg catacgattt caacttcagc
     2881 gagactgaga agtacaaaga catcgctggc ttctaccggg aggtggaaga gcaaggctat
     2941 aaggtgtcat tcgaatccgc ttctaagaag gaagtggata agctcgtgga agagggtaag
     3001 ctgtacatgt tccagatata caacaaagac ttcagcgata agagccacgg cactccaaac
     3061 ctccatacta tgtatttcaa gctgctgttt gacgagaaca accacggaca gattaggctg
     3121 tcaggaggcg cagaactctt catgcgcaga gcttcactga agaaggagga actcgttgtc
     3181 cacccagcca atagccctat agccaataag aatccagaca atcctaagaa aaccactact
     3241 ctgtcttacg atgtgtataa ggataagaga ttctctgaag atcagtacga actgcacata
     3301 cccattgcca ttaacaagtg ccctaagaac atcttcaaga ttaacacaga ggttagagtg
     3361 ctcctgaaac acgacgataa cccttatgtt ataggcattg atcgcggaga gagaaacctg
     3421 ctgtacatcg tcgtggtgga cggcaaaggc aacatcgtgg aacagtacag tctcaatgaa
     3481 atcattaaca atttcaacgg aatccgcatt aagaccgact accattctct cctcgacaag
     3541 aaggagaaag aaaggttcga agcaagacag aattggacaa gtatagagaa tatcaaagaa
     3601 ctgaaggctg ggtacatctc tcaggttgtg cacaagatat gtgagctggt ggagaagtac
     3661 gacgctgtta tcgccctcga ggacctgaat agcggcttca agaactccag ggtgaaggtg
     3721 gagaagcagg tgtatcagaa gttcgagaag atgctgatcg acaagctcaa ctatatggtg
     3781 gacaagaaat ccaatccttg cgctactggt ggagccctga agggctatca aatcaccaat
     3841 aagttcgaat ctttcaagtc tatgagcacc cagaatggct tcatcttcta catacccgca
     3901 tggctgacat ccaagattga tccctctacc ggatttgtta atctgctcaa gactaagtac
     3961 acctctattg ctgactcaaa gaagttcata tcatcatttg accgcatcat gtacgtgcca
     4021 gaagaggacc tgttcgagtt tgccctggat tacaagaatt tctctcggac tgacgccgac
     4081 tacatcaaga agtggaagct ctactcttat ggtaatcgga ttcgcatatt ccgcaatccc
     4141 aagaagaata acgtgttcga ttgggaggaa gtttgcctca ccagcgctta caaggagctg
     4201 ttcaataagt atgggattaa ctaccagcag ggcgacataa gagccctgct gtgcgaacaa
     4261 tctgataagg cattctattc ctctttcatg gcactgatgt cactgatgct gcaaatgcgc
     4321 aattccatca ccggaagaac agacgtggac tttctgatct ctcctgtcaa gaactcagat
     4381 ggcatcttct acgattcccg caactatgaa gcacaggaga atgctatcct gcctaagaat
     4441 gccgatgcaa atggagccta taacatcgcc agaaaggtcc tctgggccat aggacaattc
     4501 aagaaagctg aagatgagaa gctggacaag gtgaagatcg ccatttcaaa caaagagtgg
     4561 ctcgaatatg ctcagacctc agtgaagcat ggatcaccca agaagaaacg gaaagtgtga
     4621 gggcccttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     4681 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     4741 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4801 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4861 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4921 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4981 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5041 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5101 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5161 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5221 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5281 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5341 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5401 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5521 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5581 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5641 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5701 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5761 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     5821 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     5881 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     5941 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     6001 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     6061 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     6121 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     6181 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     6241 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg
     6301 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6361 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6421 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6541 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8761 ctgacgtc
//
