LOCUS       sequence-32360-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Hardin JW, Hu YX, McKay DB
  TITLE     Structure of the RNA binding domain of a DEAD-box helicase bound to 
            its ribosomal RNA target reveals a novel mode of recognition by an 
            RNA recognition motif.
  JOURNAL   J Mol Biol. 2010 Sep 17;402(2):412-27. doi: 
            10.1016/j.jmb.2010.07.040. Epub 2010 Jul 29.
  PUBMED    20673833
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.07.040"; journalName: "J Mol Biol"; date: 
            "2010-09-17- 17"; volume: "402"; issue: "2"; pages: "412-27"
COMMENT     Sequence Label: 23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      217..264
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(283..299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(283..299)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(296..318)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    307..323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(331..337,338..355,356..361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    376..397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(514..531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(765..784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtccaaggt atggcttttc gccatttaaa gtggtacgcg agctagccgg ccatggtccc
       61 agcctcctcg ctggcggccg gtgggcaaca tgcttcggca tggcgaatgg gacctctaga
      121 ctgtgcatcg ggtcaggact gaaaggtagc agccctgggc agttttttcc tgtgcatcgg
      181 gtcaggacct tcgggtagca gccctgggca ggatccctag cataacccct tggggcctct
      241 aaacgggtct tgaggggttt tttgaagctt ggcgtaatca tggtcatagc tgtttcctgt
      301 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      361 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      421 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg
//
