LOCUS       sequence-323601-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of D58G CARD domain variant .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 D58G-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3545..3598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3599..3601,3602..3604,3605..4318)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3599..3601,3602..3604,3605..4315)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3644..3665)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3905..3924)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4252..4273
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4332..4920
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4385..4405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4803..4814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5007..5240
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5312..5433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5349..5368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5403..5422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5473..5608
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5628..5647)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5629..5647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5657..5674)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5657..5673)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5666..5688)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5815..6270
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5902..5921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6112..6133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6296..6400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6401..6469,6470..7261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6619..6638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7432..8020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7921..7940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8174..8191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8308..8329
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8344..8349,8350..8367,8368..8374)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8382..8398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8387..8409
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8406..8422
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8406..8422
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8441..8461
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8443..8461
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8489..8715
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8716..8896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8943..9068
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9560..9793
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9978..10022
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10289..10406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     join(10741..10751,1..14)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacttccat agaaggatct
       61 cgacgccgcc accatgccag gaggagggcc agagatggat gactacatgg agacgctgaa
      121 ggatgaagag gacgccttgt gggagaatgt ggagtgtaac cggcacatgc tcagccgcta
      181 tatcaaccct gccaagctca cgccctacct gcgtcagtgt aaggtcattg atgagcagga
      241 tgaaggtgaa gtgcttaatg cccctatgct gccatccaag atcaaccgag caggccggct
      301 gttggacatt ctacatacca aggggcaaag gggctatgtg gtcttcttgg agagcctaga
      361 attttattac ccagaactgt acaaactggt gactgggaaa gagcccactc ggagattctc
      421 caccattgtg gtggaggaag gccacgaggg cctcacgcac ttcctgatga acgaggtcat
      481 caagctgcag cagcagatga aggccaagga cctgcaacgc tgcgagctgc tggccaggtt
      541 gcggcagctg gaggatgaga agaagcagat gacgctgacg cgcgtggagc tgctaacctt
      601 ccaggagcgg tactacaaga tgaaggaaga gcgggacagc tacaatgacg agctggtcaa
      661 ggtgaaggac gacaactaca acttagccat gcgctacgca cagctcagtg aggagaagaa
      721 catggcggtc atgaggagcc gagacctcca actcgagatc gatcagctaa agcaccggtt
      781 gaataagatg gaggaggaat gtaagctgga gagaaatcag tctctaaaac tgaagaatga
      841 cattgaaaat cggcccaaga aggagcaggt tctggaactg gagcgggaga atgaaatgct
      901 gaagaccaaa aaccaggagc tgcagtccat catccaggcc gggaagcgca gcctgccaga
      961 ctcagacaag gccatcctgg acatcttgga acacgaccgc aaggaggccc tggaggacag
     1021 gcaggagctg gtcaacagga tctacaacct gcaggaggag gcccgccagg cagaggagct
     1081 gcgagacaag tacctggagg agaaggagga cctggagctc aagtgctcga ccctgggaaa
     1141 ggactgtgaa atgtacaagc accgcatgaa cacggtcatg ctgcagctgg aggaggtgga
     1201 gcgggagcgg gaccaggcct tccactcccg agatgaagct cagacacagt actcgcagtg
     1261 cttaatcgaa aaggacaagt acaggaagca gatccgcgag ctggaggaga agaacgatga
     1321 gatgaggatc gagatggtgc ggcgggaggc ctgcatcgtc aacctggaga gcaagctgcg
     1381 gcgcctctcc aaggacagca acaacctgga ccagagtctg cccaggaacc tgccagtaac
     1441 catcatctct caggactttg gggatgccag ccccaggacc aatggtcaag aagctgacga
     1501 ttcttccacc tcggaggagt cacctgaaga cagcaagtac ttcctgccct accatccgcc
     1561 ccagcgcagg atgaacctga agggcatcca gctgcagaga gccaaatccc ccatcagcct
     1621 gaagcgaaca tcagattttc aagccaaggg gcacgaggaa gaaggcacgg atgccagccc
     1681 tagctcctgc ggatctctgc ccatcaccaa ctccttcacc aagatgcagc ccccccggag
     1741 ccgcagcagc atcatgtcaa tcaccgccga gcccccggga aacgactcca tcgtcagacg
     1801 ctacaaggag gacgcgcccc atcgcagcac agtcgaagaa gacaatgaca gcggcgggtt
     1861 tgacgcctta gatctggatg atgacagtca cgaacgctac tccttcggac cctcctccat
     1921 ccactcctcc tcctcctccc accaatccga gggcctggat gcctacgacc tggagcaggt
     1981 caacctcatg ttcaggaagt tctctctgga aagacccttc cggccttcgg tcacctctgt
     2041 ggggcacgtg cggggcccag ggccctcggt gcagcacacg acgctgaatg gcgacagcct
     2101 cacctcccag ctcaccctgc tggggggcaa cgcgcgaggg agcttcgtgc actcggtcaa
     2161 gcctggctct ctggccgaga aagccggcct ccgtgagggc caccagctgc tgctgctaga
     2221 aggctgcatc cgaggcgaga ggcagagtgt cccgttggac acatgcacca aagaggaagc
     2281 ccactggacc atccagaggt gcagcggccc cgtcacgctg cactacaagg tcaaccacga
     2341 agggtaccgg aagctggtga aggacatgga ggacggcctg atcacatcgg gggactcgtt
     2401 ctacatccgg ctgaacctga acatctccag ccagctggac gcctgcacca tgtccctgaa
     2461 gtgtgacgat gttgtgcacg tccgtgacac catgtaccag gacaggcacg agtggctgtg
     2521 cgcgcgggtc gaccctttca cagaccatga cctggatatg ggcaccatac ccagctacag
     2581 ccgagcccag cagctcctcc tggtgaaact gcagcgcctg atgcaccgag gcagccggga
     2641 ggaggtagac ggcacccacc acaccctgcg ggcactccgg aacaccctgc agccagaaga
     2701 agcgctttca acaagcgacc cccgggtcag cccccgtctc tcgcgagcaa gcttcctttt
     2761 tggccagctc cttcagttcg tcagcaggtc cgagaacaag tataagcgga tgaacagcaa
     2821 cgagcgggtc cgcatcatct cggggagtcc gctagggagc ctggcccggt cctcgctgga
     2881 cgccaccaag ctcttgactg agaagcagga agagctggac cctgagagcg agctgggcaa
     2941 gaacctcagc ctcatcccct acagcctggt acgcgccttc tactgcgagc gccgccggcc
     3001 cgtgctcttc acacccaccg tgctggccaa gacgctggtg cagaggctgc tcaactcggg
     3061 aggtgccatg gagttcacca tctgcaagtc agatatcgtc acaagagatg agttcctcag
     3121 aaggcagaag acggagacca tcatctactc ccgagagaag aaccccaacg cgttcgaatg
     3181 catcgcccct gccaacattg aagctgtggc cgccaagaac aagcactgcc tgctggaggc
     3241 tgggatcggc tgcacaagag acttgatcaa gtccaacatc taccccatcg tgctcttcat
     3301 ccgggtgtgt gagaagaaca tcaagaggtt cagaaagctg ctgccccgac ctgagacgga
     3361 ggaggagttc ctgcgcgtgt gccggctgaa ggagaaggag ctggaggccc tgccgtgcct
     3421 gtacgccacg gtggaacctg acatgtgggg cagcgtagag gagctgctcc gcgttgtcaa
     3481 ggacaagatc ggcgaggagc agcgcaagac catctgggtg gacgaggacc agctgggatc
     3541 cggtgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc cgggccccat
     3601 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     3661 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     3721 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     3781 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     3841 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     3901 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     3961 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4021 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     4081 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4201 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4261 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgaat
     4321 ctagagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4381 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4441 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4501 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4561 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4621 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4681 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4741 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4801 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4861 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4921 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     4981 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5041 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5101 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5161 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5221 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     5281 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     5341 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5401 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     5461 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5521 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5581 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     5641 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5701 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5761 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     5821 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5881 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5941 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6001 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6061 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6121 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6181 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6241 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6361 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6421 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6481 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6541 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6601 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6661 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6721 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6781 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6841 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6901 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6961 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7021 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7081 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7141 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7201 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7261 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7321 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7381 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7441 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7501 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7561 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7621 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7681 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7741 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7801 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7861 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7921 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7981 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8041 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8101 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8161 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8221 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8281 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8341 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8401 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     8461 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     8521 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     8581 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     8641 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     8701 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8881 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8941 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9001 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9061 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9121 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9181 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9241 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9301 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9361 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ctgatcttca
     9421 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9481 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9541 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9601 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9661 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     9721 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9781 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9841 cttggaatgc tagttggagt aatgaatctc tggaacagat ttggaatcac acgacctgga
     9901 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9961 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    10021 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    10081 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    10141 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    10201 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    10261 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
    10321 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10381 aacaaattac aaaaattcaa aattttatcg acgcgtagga acagagaaac aggagaatat
    10441 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagtt
    10501 ggaacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
    10561 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
    10621 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
    10681 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctct atataagcag
    10741 agctcgttta g
//
