LOCUS       sequence-323602-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of R72Q CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 R72Q-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3542..3595
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3596..3598,3599..3601,3602..4315)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3596..3598,3599..3601,3602..4312)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3641..3662)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3902..3921)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4249..4270
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4329..4917
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4382..4402)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4800..4811)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5004..5237
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5309..5430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5346..5365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5400..5419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5470..5605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5625..5644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5626..5644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5654..5671)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5654..5670)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5663..5685)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5812..6267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5899..5918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6109..6130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6293..6397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6398..6466,6467..7258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6616..6635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7429..8017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7918..7937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8171..8188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8305..8326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8341..8346,8347..8364,8365..8371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8379..8395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8384..8406
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8403..8419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8403..8419
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8438..8458
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8440..8458
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8486..8712
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8713..8893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8940..9065
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9557..9790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9975..10019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10286..10403
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     join(10738..10751,1..11)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cttccataga aggatctcga
       61 cgccgccacc atgccaggag gagggccaga gatggatgac tacatggaga cgctgaagga
      121 tgaagaggac gccttgtggg agaatgtgga gtgtaaccgg cacatgctca gccgctatat
      181 caaccctgcc aagctcacgc cctacctgcg tcagtgtaag gtcattgatg agcaggatga
      241 agatgaagtg cttaatgccc ctatgctgcc atccaagatc aaccaggcag gccggctgtt
      301 ggacattcta cataccaagg ggcaaagggg ctatgtggtc ttcttggaga gcctagaatt
      361 ttattaccca gaactgtaca aactggtgac tgggaaagag cccactcgga gattctccac
      421 cattgtggtg gaggaaggcc acgagggcct cacgcacttc ctgatgaacg aggtcatcaa
      481 gctgcagcag cagatgaagg ccaaggacct gcaacgctgc gagctgctgg ccaggttgcg
      541 gcagctggag gatgagaaga agcagatgac gctgacgcgc gtggagctgc taaccttcca
      601 ggagcggtac tacaagatga aggaagagcg ggacagctac aatgacgagc tggtcaaggt
      661 gaaggacgac aactacaact tagccatgcg ctacgcacag ctcagtgagg agaagaacat
      721 ggcggtcatg aggagccgag acctccaact cgagatcgat cagctaaagc accggttgaa
      781 taagatggag gaggaatgta agctggagag aaatcagtct ctaaaactga agaatgacat
      841 tgaaaatcgg cccaagaagg agcaggttct ggaactggag cgggagaatg aaatgctgaa
      901 gaccaaaaac caggagctgc agtccatcat ccaggccggg aagcgcagcc tgccagactc
      961 agacaaggcc atcctggaca tcttggaaca cgaccgcaag gaggccctgg aggacaggca
     1021 ggagctggtc aacaggatct acaacctgca ggaggaggcc cgccaggcag aggagctgcg
     1081 agacaagtac ctggaggaga aggaggacct ggagctcaag tgctcgaccc tgggaaagga
     1141 ctgtgaaatg tacaagcacc gcatgaacac ggtcatgctg cagctggagg aggtggagcg
     1201 ggagcgggac caggccttcc actcccgaga tgaagctcag acacagtact cgcagtgctt
     1261 aatcgaaaag gacaagtaca ggaagcagat ccgcgagctg gaggagaaga acgatgagat
     1321 gaggatcgag atggtgcggc gggaggcctg catcgtcaac ctggagagca agctgcggcg
     1381 cctctccaag gacagcaaca acctggacca gagtctgccc aggaacctgc cagtaaccat
     1441 catctctcag gactttgggg atgccagccc caggaccaat ggtcaagaag ctgacgattc
     1501 ttccacctcg gaggagtcac ctgaagacag caagtacttc ctgccctacc atccgcccca
     1561 gcgcaggatg aacctgaagg gcatccagct gcagagagcc aaatccccca tcagcctgaa
     1621 gcgaacatca gattttcaag ccaaggggca cgaggaagaa ggcacggatg ccagccctag
     1681 ctcctgcgga tctctgccca tcaccaactc cttcaccaag atgcagcccc cccggagccg
     1741 cagcagcatc atgtcaatca ccgccgagcc cccgggaaac gactccatcg tcagacgcta
     1801 caaggaggac gcgccccatc gcagcacagt cgaagaagac aatgacagcg gcgggtttga
     1861 cgccttagat ctggatgatg acagtcacga acgctactcc ttcggaccct cctccatcca
     1921 ctcctcctcc tcctcccacc aatccgaggg cctggatgcc tacgacctgg agcaggtcaa
     1981 cctcatgttc aggaagttct ctctggaaag acccttccgg ccttcggtca cctctgtggg
     2041 gcacgtgcgg ggcccagggc cctcggtgca gcacacgacg ctgaatggcg acagcctcac
     2101 ctcccagctc accctgctgg ggggcaacgc gcgagggagc ttcgtgcact cggtcaagcc
     2161 tggctctctg gccgagaaag ccggcctccg tgagggccac cagctgctgc tgctagaagg
     2221 ctgcatccga ggcgagaggc agagtgtccc gttggacaca tgcaccaaag aggaagccca
     2281 ctggaccatc cagaggtgca gcggccccgt cacgctgcac tacaaggtca accacgaagg
     2341 gtaccggaag ctggtgaagg acatggagga cggcctgatc acatcggggg actcgttcta
     2401 catccggctg aacctgaaca tctccagcca gctggacgcc tgcaccatgt ccctgaagtg
     2461 tgacgatgtt gtgcacgtcc gtgacaccat gtaccaggac aggcacgagt ggctgtgcgc
     2521 gcgggtcgac cctttcacag accatgacct ggatatgggc accataccca gctacagccg
     2581 agcccagcag ctcctcctgg tgaaactgca gcgcctgatg caccgaggca gccgggagga
     2641 ggtagacggc acccaccaca ccctgcgggc actccggaac accctgcagc cagaagaagc
     2701 gctttcaaca agcgaccccc gggtcagccc ccgtctctcg cgagcaagct tcctttttgg
     2761 ccagctcctt cagttcgtca gcaggtccga gaacaagtat aagcggatga acagcaacga
     2821 gcgggtccgc atcatctcgg ggagtccgct agggagcctg gcccggtcct cgctggacgc
     2881 caccaagctc ttgactgaga agcaggaaga gctggaccct gagagcgagc tgggcaagaa
     2941 cctcagcctc atcccctaca gcctggtacg cgccttctac tgcgagcgcc gccggcccgt
     3001 gctcttcaca cccaccgtgc tggccaagac gctggtgcag aggctgctca actcgggagg
     3061 tgccatggag ttcaccatct gcaagtcaga tatcgtcaca agagatgagt tcctcagaag
     3121 gcagaagacg gagaccatca tctactcccg agagaagaac cccaacgcgt tcgaatgcat
     3181 cgcccctgcc aacattgaag ctgtggccgc caagaacaag cactgcctgc tggaggctgg
     3241 gatcggctgc acaagagact tgatcaagtc caacatctac cccatcgtgc tcttcatccg
     3301 ggtgtgtgag aagaacatca agaggttcag aaagctgctg ccccgacctg agacggagga
     3361 ggagttcctg cgcgtgtgcc ggctgaagga gaaggagctg gaggccctgc cgtgcctgta
     3421 cgccacggtg gaacctgaca tgtggggcag cgtagaggag ctgctccgcg ttgtcaagga
     3481 caagatcggc gaggagcagc gcaagaccat ctgggtggac gaggaccagc tgggatccgg
     3541 tgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatccgg gccccatggt
     3601 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3661 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3721 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3781 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3841 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3901 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3961 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4021 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     4081 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4141 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4201 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4261 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgaatcta
     4321 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4381 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4441 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4501 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4561 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4621 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4681 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4741 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4801 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4861 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4921 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4981 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5041 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5101 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5161 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5221 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     5281 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     5341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5401 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5521 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5581 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     5641 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5701 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5761 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     5821 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5881 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5941 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6001 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6061 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6121 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6181 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6241 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     6301 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6361 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6421 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6481 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6541 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6601 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6661 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6721 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6781 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6841 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6901 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6961 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7021 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7081 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7141 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7201 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7261 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7321 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7561 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8041 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8101 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8161 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8221 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8281 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8341 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8401 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     8461 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     8521 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     8581 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     8641 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     8701 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8881 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8941 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     9001 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     9061 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     9121 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     9181 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     9241 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     9301 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     9361 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccctg atcttcagac
     9421 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9481 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9541 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9601 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9661 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9721 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9781 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     9841 ggaatgctag ttggagtaat gaatctctgg aacagatttg gaatcacacg acctggatgg
     9901 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     9961 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10021 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    10081 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    10141 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    10201 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    10261 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
    10321 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10381 aaattacaaa aattcaaaat tttatcgacg cgtaggaaca gagaaacagg agaatatggg
    10441 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga
    10501 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
    10561 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag
    10621 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
    10681 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctctata taagcagagc
    10741 tcgtttagtg a
//
