LOCUS       sequence-323606-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of K41R CARD domain variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, 
            Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers 
            CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, 
            Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper 
            MA, Starita LM, Rawlings DJ, Snow AL, James RG
  TITLE     Multiplexed Functional Assessment of Genetic Variants in CARD11.
  JOURNAL   Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015.
  PUBMED    33202260
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 
            10.1016/j.ajhg.2020.10.015."
COMMENT     Sequence Label: pRRL-MND-hCard11 K41R-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1248..1301
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1302..1304,1305..1307,1308..2021)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1302..1304,1305..1307,1308..2018)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1347..1368)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1608..1627)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1955..1976
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2035..2623
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2088..2108)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2506..2517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2710..2943
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3015..3136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3052..3071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3106..3125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3176..3311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3331..3350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3332..3350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3360..3377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3360..3376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3369..3391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3518..3973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3605..3624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3815..3836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3999..4103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4104..4172,4173..4964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4322..4341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5135..5723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5624..5643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5877..5894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6011..6032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6047..6052,6053..6070,6071..6077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6085..6101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6090..6112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6109..6125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6109..6125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6144..6164
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6146..6164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6192..6418
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6419..6599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6646..6771
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7263..7496
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7681..7725
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7992..8109
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     8444..8468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggtgcagcgg ccccgtcacg ctgcactaca aggtcaacca cgaagggtac cggaagctgg
       61 tgaaggacat ggaggacggc ctgatcacat cgggggactc gttctacatc cggctgaacc
      121 tgaacatctc cagccagctg gacgcctgca ccatgtccct gaagtgtgac gatgttgtgc
      181 acgtccgtga caccatgtac caggacaggc acgagtggct gtgcgcgcgg gtcgaccctt
      241 tcacagacca tgacctggat atgggcacca tacccagcta cagccgagcc cagcagctcc
      301 tcctggtgaa actgcagcgc ctgatgcacc gaggcagccg ggaggaggta gacggcaccc
      361 accacaccct gcgggcactc cggaacaccc tgcagccaga agaagcgctt tcaacaagcg
      421 acccccgggt cagcccccgt ctctcgcgag caagcttcct ttttggccag ctccttcagt
      481 tcgtcagcag gtccgagaac aagtataagc ggatgaacag caacgagcgg gtccgcatca
      541 tctcggggag tccgctaggg agcctggccc ggtcctcgct ggacgccacc aagctcttga
      601 ctgagaagca ggaagagctg gaccctgaga gcgagctggg caagaacctc agcctcatcc
      661 cctacagcct ggtacgcgcc ttctactgcg agcgccgccg gcccgtgctc ttcacaccca
      721 ccgtgctggc caagacgctg gtgcagaggc tgctcaactc gggaggtgcc atggagttca
      781 ccatctgcaa gtcagatatc gtcacaagag atgagttcct cagaaggcag aagacggaga
      841 ccatcatcta ctcccgagag aagaacccca acgcgttcga atgcatcgcc cctgccaaca
      901 ttgaagctgt ggccgccaag aataagcact gcctgctgga ggctgggatc ggctgcacaa
      961 gagacttgat caagtccaac atctacccca tcgtgctctt catccgggtg tgtgagaaga
     1021 acatcaagag gttcagaaag ctgctgcccc gacctgagac ggaggaggag ttcctgcgcg
     1081 tgtgccggct gaaggagaag gagctggagg ccctgccgtg cctgtacgcc acggtggaac
     1141 ctgacatgtg gggcagcgta gaggagctgc tccgcgttgt caaggacaag atcggcgagg
     1201 agcagcgcaa gaccatctgg gtggacgagg accagctggg atccggtgag ggcagaggaa
     1261 gtcttctaac atgcggtgac gtggaggaga atccgggccc catggtgagc aagggcgagg
     1321 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1381 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1441 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1501 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1561 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1621 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1681 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1741 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1801 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1861 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1921 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1981 ccgccgggat cactctcggc atggacgagc tgtacaagtg aatctagagt cgacaatcaa
     2041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2401 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     2461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg
     2641 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     2701 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     2761 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     2821 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     2881 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2941 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3001 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3061 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3121 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3181 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3241 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3301 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     3361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3481 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     3541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3961 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4081 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4141 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4201 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4261 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4321 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4381 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4441 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4501 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4561 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4621 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5281 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5881 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5941 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6001 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6061 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6121 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     6181 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     6241 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     6301 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     6361 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     6421 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6481 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6541 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6601 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6661 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6721 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     6781 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     6841 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     6901 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     6961 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7021 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     7081 aacaaaagta agaccaccgc acagcaagcg gccctgatct tcagacctgg aggaggagat
     7141 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7201 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7261 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     7321 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     7381 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     7441 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     7501 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     7561 agtaatgaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     7621 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     7681 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     7741 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     7801 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     7861 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     7921 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     7981 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     8041 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     8101 caaaatttta tcgacgcgta ggaacagaga aacaggagaa tatgggccaa acaggatatc
     8161 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gttggaacag cagaatatgg
     8221 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     8281 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     8341 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     8401 cttctgttcg cgcgcttctg ctccccgagc tctatataag cagagctcgt ttagtgaacc
     8461 gtcagatcgc ctggagacgc catccacgct gttttgactt ccatagaagg atctcgacgc
     8521 cgccaccatg ccaggaggag ggccagagat ggatgactac atggagacgc tgaaggatga
     8581 agaggacgcc ttgtgggaga atgtggagtg taaccggcac atgctcagcc gctatatcaa
     8641 ccctgccagg ctcacgccct acctgcgtca gtgtaaggtc attgatgagc aggatgaaga
     8701 tgaagtgctt aatgccccta tgctgccatc caagatcaac cgagcaggcc ggctgttgga
     8761 cattctacat accaaggggc aaaggggcta tgtggtcttc ttggagagcc tagaatttta
     8821 ttacccagaa ctgtacaaac tggtgactgg gaaagagccc actcggagat tctccaccat
     8881 tgtggtggag gaaggccacg agggcctcac gcacttcctg atgaacgagg tcatcaagct
     8941 gcagcagcag atgaaggcca aggacctgca acgctgcgag ctgctggcca ggttgcggca
     9001 gctggaggat gagaagaagc agatgacgct gacgcgcgtg gagctgctaa ccttccagga
     9061 gcggtactac aagatgaagg aagagcggga cagctacaat gacgagctgg tcaaggtgaa
     9121 ggacgacaac tacaacttag ccatgcgcta cgcacagctc agtgaggaga agaacatggc
     9181 ggtcatgagg agccgagacc tccaactcga gatcgatcag ctaaagcacc ggttgaataa
     9241 gatggaggag gaatgtaagc tggagagaaa tcagtctcta aaactgaaga atgacattga
     9301 aaatcggccc aagaaggagc aggttctgga actggagcgg gagaatgaaa tgctgaagac
     9361 caaaaaccag gagctgcagt ccatcatcca ggccgggaag cgcagcctgc cagactcaga
     9421 caaggccatc ctggacatct tggaacacga ccgcaaggag gccctggagg acaggcagga
     9481 gctggtcaac aggatctaca acctgcagga ggaggcccgc caggcagagg agctgcgaga
     9541 caagtacctg gaggagaagg aggacctgga gctcaagtgc tcgaccctgg gaaaggactg
     9601 tgaaatgtac aagcaccgca tgaacacggt catgctgcag ctggaggagg tggagcggga
     9661 gcgggaccag gccttccact cccgagatga agctcagaca cagtactcgc agtgcttaat
     9721 cgaaaaggac aagtacagga agcagatccg cgagctggag gagaagaacg atgagatgag
     9781 gatcgagatg gtgcggcggg aggcctgcat cgtcaacctg gagagcaagc tgcggcgcct
     9841 ctccaaggac agcaacaacc tgaaccagag tctgcccagg aacctgccag taaccatcat
     9901 ctctcaggac tttggggatg ccagccccag gaccaatggt caagaagctg acgattcttc
     9961 cacctcggag gagtcacctg aagacagcaa gtacttcctg ccctaccatc cgccccagcg
    10021 caggatgaac ctgaagggca tccagctgca gagagccaaa tcccccatca gcctgaagcg
    10081 aacatcagat tttcaagcca aggggcacga ggaagaaggc acggatgcca gccctagctc
    10141 ctgcggatct ctgcccatca ccaactcctt caccaagatg cagccccccc ggagccgcag
    10201 cagcatcatg tcaatcaccg ccgagccccc gggaaacgac tccatcgtca gacgctacaa
    10261 ggaggacgcg ccccatcgca gcacagtcga agaagacaat gacagcggcg ggtttgacgc
    10321 cttagatctg gatgatgaca gtcacgaacg ctactccttc ggaccctcct ccatccactc
    10381 ctcctcctcc tcccaccaat ccgagggcct ggatgcctac gacctggagc aggtcaacct
    10441 catgttcagg aagttctctc tggaaagacc cttccggcct tcggtcacct ctgtggggca
    10501 cgtgcggggc ccagggccct cggtgcagca cacgacgctg aatggcgaca gcctcacctc
    10561 ccagctcacc ctgctggggg gcaacgcgcg agggagcttc gtgcactcgg tcaagcctgg
    10621 ctctctggcc gagaaagccg gcctccgtga gggccaccag ctgctgctgc tagaaggctg
    10681 catccgaggc gagaggcaga gtgtcccgtt ggacacatgc accaaagagg aagcccactg
    10741 gaccatccag a
//
