LOCUS       sequence-323608-        6494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of SomArchon-GFP under the Syn promoter, in 
            floxed/reversed (Cre-dependent) manner..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG, 
            Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa 
            E, Sabatini BL, Fu Z, Boyden ES, Han X
  TITLE     Population imaging of neural activity in awake behaving mice.
  JOURNAL   Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1. 
            Epub 2019 Oct 9.
  PUBMED    31597963
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1641-1"; journalName: "Nature"; date: "2019-10";
            volume: "574"; issue: "7778"; pages: "413-417"
COMMENT     Sequence Label: AAV-Syn-FLEX-rc [SomArchon-GFP]
FEATURES             Location/Qualifiers
     source          1..6494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   47..176
                     /label=AAV2 ITR
     promoter        279..726
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    742..775
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    838..871
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(1125..1835,1836..1838))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1125..1835,1836..1838))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1167..1188)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1516..1535
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1775..1796
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2702..2711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2722..2755)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2826..2859)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2892..3480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2945..2965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3363..3374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3521..3728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3898..4027
                     /label=AAV2 ITR
     rep_origin      complement(4264..4852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4344..4363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5023..5814,5815..5883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5646..5665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5884..5988)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6014..6469)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6151..6172)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6363..6382
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc
       61 gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc
      121 agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta
      181 gttaatgatt aacccgccat gctacttatc tacgtagcca tgctctagga agatcgtagg
      241 ttaattaagt gtctagactg cagagggccc tgcgtatgag tgcaagtggg ttttaggacc
      301 aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact ggacaagcac
      361 ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg aaacaggatg
      421 cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc gcccccgcct
      481 ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact cgccggtccc
      541 ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga
      601 ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg ctgcggcgcc
      661 ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga
      721 gcgcagtcga gaagctagcc cataacttcg tataaagtat cctatacgaa gttatatcaa
      781 aataggaaga ccaatgcttc accatcgacc cgaattgcca agcatcacca tcgacccata
      841 acttcgtata atgtatgcta tacgaagtta tactagaatt cttacacctc gttctcgtag
      901 cagaacttgt agaacctggt ggcctcaggg aagtcggtgg cgcagctgat gaagctgtcg
      961 atgctgctca tgctcctcat gtcgatcacg ccctcggtcc tggcaggcag aggggccaca
     1021 gggctaggca tgctgctcat ctccagctcc tcaggaggct tgctctgagg ggccatctcc
     1081 ttggtgttca ggataggctg gctctgggta cctccgctcc cacccttgta cagctcgtcc
     1141 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     1201 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     1261 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     1321 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     1381 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     1441 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     1501 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     1561 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     1621 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     1681 agcttgccgg tggtgcaaat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     1741 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     1801 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca cgttgatgtc gatctggtcc
     1861 agggggatgt actcgccctc gctggtgatc ctgctcttgg cggccgcctt gctcactgct
     1921 actaccgggc ggtcggcggc accggtatca gctccagctg aaggttcagg tgcttctgta
     1981 tcttgcagaa tggctcttga cctgagcagg ataaaaccga agcccacctt ggcggtgacg
     2041 tccagcacca tgaacagcaa agtttcgatt cccaggccca caactccggc accctcggtg
     2101 ccgataatcc agatgatagg gtaagcagtc cacaggacca gaaccagtgc ggtgaggatg
     2161 ttgaaggttg aggcaacctc tgggcctctc tccttagcgg ctgaccgcag agaagtggcc
     2221 aggacataca gaacgacgat catacagata gttgagaaca gccaccaaga gtatctggca
     2281 atggcagtat gtgacagtgt tcctatcagg cctgtgacaa tcatcagagc atcgacgcca
     2341 acgagagtgc cgatggtcac tctatcaact ttggccagga gtgccaggtg cagcagcaac
     2401 agtggtgtag tgaacagcca ctgtgcgtat cttgcgtagt agatgttcag catctctcca
     2461 cctactggca cctctgtgag gccaatacca aagaacattg acagataagc tgcactggca
     2521 ataccgctga ccaggattgt cactgcgtag tactcccgtg catccttgtc agtctctccc
     2581 cacgctctaa ccaggaagta gaaggttccg atcagcatca gcagtgtacc aattccgagc
     2641 cacagacttt caggtctacc atctccgagg aggtcataac cagcctgcag tgcgatagaa
     2701 accatggtgg cggatcctag tataacttcg tataggatac tttatacgaa gttatcattg
     2761 ggattcttcc tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc
     2821 gacccataac ttcgtatagc atacattata cgaagttatg tccctcgaag aggttcgcga
     2881 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2941 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3001 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3061 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3121 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3181 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3241 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3301 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3361 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3421 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3481 atcgataccg tcgacccggg cggccgctca taaagctcga ctgtgccttc tagttgccag
     3541 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3601 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3661 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3721 gctggggatg cggtccggac tgtacaattg cattcatttt atgtttcagg ttcaggggga
     3781 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     3841 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     3901 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3961 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4021 cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6001 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     6061 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6121 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6181 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6241 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6301 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6361 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6421 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     6481 ttcaggctgc gcaa
//
