LOCUS       sequence-323614-        7048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn 
            promoter, in floxed/reversed (Cre-dependent) manner..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, 
            Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, 
            Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y,
            Tye KM, Roska B, Cardin JA, Boyden ES
  TITLE     Noninvasive optical inhibition with a red-shifted microbial 
            rhodopsin.
  JOURNAL   Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014
            Jul 6.
  PUBMED    24997763
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3752"; 
            journalName: "Nat Neurosci"; date: "2014-08"; volume: "17"; issue: 
            "8"; pages: "1123-9"
COMMENT     Sequence Label: pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2]
FEATURES             Location/Qualifiers
     source          1..7048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2090)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2281..2300
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2459..2588
                     /label=AAV2 ITR
     promoter        2691..3138
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    3154..3187
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3250..3283
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5688..5721)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5792..5825)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5858..6446
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5911..5931)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6329..6340)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6487..6694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6864..6993
                     /label=AAV2 ITR
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa gtgtctagac tgcagagggc cctgcgtatg agtgcaagtg
     2701 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca
     2761 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
     2821 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
     2881 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
     2941 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
     3001 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
     3061 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
     3121 tcgtgcctga gagcgcagtc gagaagaatt cccataactt cgtataaagt atcctatacg
     3181 aagttatatc aaaataggaa gaccaatgct tcaccatcga cccgaattgc caagcatcac
     3241 catcgaccca taacttcgta taatgtatgc tatacgaagt tatactagct agctcattac
     3301 acttcattct cgtagcagaa cttatacagc tcatccatgc cgtacagaaa cagatggtgg
     3361 cgtccttcag acctctcata ctgttccaca atggtgtagt cttcattgtg ggatgtgata
     3421 tccagcttgg tgtccacata gtaatagccg ggcagctgca ctggtttctt ggccatgtag
     3481 atggttttga actccaccag gtagtggccg ccgtctttca gcttcagggc ctggtggatt
     3541 tcgcccttca gcactccgtc tctaggatac agccgttcag tgctggcctc ccaccccatt
     3601 gttttcttct gcatcacggg cccatcgggt ggaaaattgg tgccgcgcat tttgactttg
     3661 tagatcagag tgccatcctg cagagaggag tcctgtgtca cggtcaccag gcctccatct
     3721 tcaaaattca tcactctctc ccacttaaag ccctctggga aagacagttt cttgtaatca
     3781 ggaatgtcag cgggatgctt gacataggcc ttacttccgt acataaactg aggagacagg
     3841 atatcccaag cgaaaggcag tggtcctccc ttggtcactt tcagcttggc agtctgtgtg
     3901 ccctcgtaag gcctcccttc gccctctcct tcaatctcaa attcgtgccc attcatgctg
     3961 ccctccatgc gcaccttaaa cctcatgaac tctttgatca cagccatatt gttatcctcg
     4021 cttgaggcgg ttccactaga ccctgagccg gtacttccag tcccatggcc caggaacaga
     4081 tggtgtctcc cctcgctccg ttcatactgc tccacaatag tgtagtcctc gttgtgtgaa
     4141 gtgatatcca gttttgtgtc cacgtaatag tagcctggca gctgcacagg tttcttggcc
     4201 atgtagattg tcttgaactc caccaggtaa tgtcccccat ctttcagctt cagagcctgg
     4261 tggatttctc ctttcagcac cccgtcgcgt ggatacagcc tttcggtgga ggcctcccat
     4321 cccatagttt tcttctgcat cacaggtcca tcagggggaa aatttgtccc tctcattttc
     4381 accttgtaga tcagggtccc atcctgcagg gagctgtcct gggtcacagt caccaggcct
     4441 ccgtcctcga agttcatcac tctttcccac ttaaacccct cggggaagct cagtttctta
     4501 taatcgggaa tgtcagctgg atgtttcaca taggccttgg agccgtacat aaactgtggg
     4561 ctcaggatgt cccaagcgaa tggcagaggc ccgccctttg tcactttcag cttggctgtc
     4621 tgggtgccct cgtagggccg tccctctcct tctccctcaa tttcgaactc gtggccgttc
     4681 atgcttcctt ccattctcac cttaaaccgc atgaactctt tgatcacttc ctcgccctta
     4741 ctcactgccc ctgcaggtgc aacattgatg tctatttgat ccagagggat atactcccct
     4801 tcagaagtaa ttctggattt tactaccggt cgatcgtcgg ctggagcagc tcctccggac
     4861 ccgattccca ttccactccc agacactgtg ccttcattgg cagccaccca tctcagcagc
     4921 agaaaggcga acacgtattt agccaggatg tccagtccgg agtatcccca gctggtcact
     4981 cccacactca gcagggccac tccctcagag cccagagcga acaggatagg gtatcccagc
     5041 cacagcacca cggtcagaat cctcagagtg ccaaagattt cactggtccc agcagcctca
     5101 gcatcagctg gccactgcac cagcagcaca tacagcacgg ccacaaagaa agcacaagaa
     5161 attccgtaga acacccagcg cagcagatgg gagctagtga tcagggcagc ggccagtcct
     5221 gtcacgcaca tgccaatgtc cattgtgatg gcggtgaaca ggctggcaat atcggtgtcg
     5281 gccagcagtc ccagagccag caggatcatg ggagtggaga aagtccatgt cagataccgg
     5341 ccccatgggc tcagcacctc ctgtccggcc agagcgtgtc caggtggcat ctgcaggaag
     5401 cccacagtca gcccactggc cagtccagcg taactagaaa tagacaccag tggcaccagc
     5461 attgtggcca cccagatcag cttagctcta ggggattcca gatccctccc catggccaca
     5521 aacagcagga tgaccactcc ggccagagca atgttcaccc agatggagct attcagcagg
     5581 aagttggact ggatctcctg cagcacgtcg ctctgtgtgg cctgcagcac ggtagtggct
     5641 gtggtgctca cggcggtcat ggtggcggcg cggcctaggt acctagtata acttcgtata
     5701 ggatacttta tacgaagtta tcattgggat tcttcctatt ttgatccaag catcaccatc
     5761 gaccctctag tccagatctc accatcgacc cataacttcg tatagcatac attatacgaa
     5821 gttatgtccc tcgaagaggt tcgcgaagct tatcgataat caacctctgg attacaaaat
     5881 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5941 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6001 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6061 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6121 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6181 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6241 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6301 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6361 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6421 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cccgggcggc cgctcataaa
     6481 gctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6541 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6601 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6661 aggattggga agacaatagc aggcatgctg gggatgcggt ccggactgta caattgcatt
     6721 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
     6781 tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca
     6841 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     6901 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     6961 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg
     7021 gggagaggcg gtttgcgtat tgggcgct
//
