LOCUS       sequence-32362-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Hardin JW, Hu YX, McKay DB
  TITLE     Structure of the RNA binding domain of a DEAD-box helicase bound to 
            its ribosomal RNA target reveals a novel mode of recognition by an 
            RNA recognition motif.
  JOURNAL   J Mol Biol. 2010 Sep 17;402(2):412-27. doi: 
            10.1016/j.jmb.2010.07.040. Epub 2010 Jul 29.
  PUBMED    20673833
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.07.040"; journalName: "J Mol Biol"; date: 
            "2010-09-17- 17"; volume: "402"; issue: "2"; pages: "412-27"
COMMENT     Sequence Label: 23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      207..254
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(273..289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(273..289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(286..308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    297..313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(321..327,328..345,346..351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    366..387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(504..521)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(755..774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtatggct gttcgccatt taaagtgatg agtcagccgg ccatggtccc agcctcctcg
       61 ctggcggccg gtgggcaaca tgcttcggca tggcgaatgg gacctctaga ctgtgcatcg
      121 ggtcaggact gaaaggtagc agccctgggc agttttttcc tgtgcatcgg gtcaggacct
      181 tcgggtagca gccctgggca ggatccctag cataacccct tggggcctct aaacgggtct
      241 tgaggggttt tttgaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgg
//
