LOCUS       sequence-323632-       10795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length human ACE2 protein, along with TagBFP reporter
            and Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell
            JF, Neelamegham S
  TITLE     Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan 
            elaboration.
  JOURNAL   Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.
  PUBMED    33103998
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-26"; volume: "9. pii: 61552. doi"; pages: " 
            10.7554/eLife.61552"
COMMENT     Sequence Label: ACE2
FEATURES             Location/Qualifiers
     source          1..10795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        59..362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        363..566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3009..3065
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3093..3689
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3093..3112)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3589..3609
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3708..3761
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3762..4463
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4523..4543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4941..4952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5404..5421)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5410..5634
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5660..5680)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5660..5678)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5699..5715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5699..5715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5712..5734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5723..5739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5747..5753,5754..5771,5772..5777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5792..5813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5930..5947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6101..6689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6181..6200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6860..7651,7652..7720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7483..7502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7721..7825)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7851..8306)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7988..8009)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8200..8219
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8433..8455
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8447..8464
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8448..8464
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8474..8493
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8474..8492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        8808..9006
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9024..9204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9251..9376
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9875..10108
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10293..10337
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10635..10752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggccctac gcgtcaccgg gtcttcgaga agacacgttt ggatcctatg gagttccgcg
       61 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      121 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      181 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      241 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      301 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      361 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      421 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      481 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      541 ggtgggaggt ctatataagc agagctgaat tcgccaccat gtcaagctct tcctggctcc
      601 ttctcagcct tgttgctgta actgctgctc agtccaccat tgaggaacag gccaagacat
      661 ttttggacaa gtttaaccac gaagccgaag acctgttcta tcaaagttca cttgcttctt
      721 ggaattataa caccaatatt actgaagaga atgtccaaaa catgaataat gctggggaca
      781 aatggtctgc ctttttaaag gaacagtcca cacttgccca aatgtatcca ctacaagaaa
      841 ttcagaatct cacagtcaag cttcagctgc aggctcttca gcaaaatggg tcttcagtgc
      901 tctcagaaga caagagcaaa cggttgaaca caattctaaa tacaatgagc accatctaca
      961 gtactggaaa agtttgtaac ccagataatc cacaagaatg cttattactt gaaccaggtt
     1021 tgaatgaaat aatggcaaac agtttagact acaatgagag gctctgggct tgggaaagct
     1081 ggagatctga ggtcggcaag cagctgaggc cattatatga agagtatgtg gtcttgaaaa
     1141 atgagatggc aagagcaaat cattatgagg actatgggga ttattggaga ggagactatg
     1201 aagtaaatgg ggtagatggc tatgactaca gccgcggcca gttgattgaa gatgtggaac
     1261 atacctttga agagattaaa ccattatatg aacatcttca tgcctatgtg agggcaaagt
     1321 tgatgaatgc ctatccttcc tatatcagtc caattggatg cctccctgct catttgcttg
     1381 gtgatatgtg gggtagattt tggacaaatc tgtactcttt gacagttccc tttggacaga
     1441 aaccaaacat agatgttact gatgcaatgg tggaccaggc ctgggatgca cagagaatat
     1501 tcaaggaggc cgagaagttc tttgtatctg ttggtcttcc taatatgact caaggattct
     1561 gggaaaattc catgctaacg gacccaggaa atgttcagaa agcagtctgc catcccacag
     1621 cttgggacct ggggaagggc gacttcagga tccttatgtg cacaaaggtg acaatggacg
     1681 acttcctgac agctcatcat gagatggggc atatccagta tgatatggca tatgctgcac
     1741 aaccttttct gctaagaaat ggagctaatg aaggattcca tgaagctgtt ggggaaatca
     1801 tgtcactttc tgcagccaca cctaagcatt taaaatccat tggtcttctg tcacccgatt
     1861 ttcaagaaga caatgaaaca gaaataaact tcctgctcaa acaagcactc acgattgttg
     1921 ggactctgcc atttacttac atgttagaga agtggaggtg gatggtcttt aaaggggaaa
     1981 ttcccaaaga ccagtggatg aaaaagtggt gggagatgaa gcgagagata gttggggtgg
     2041 tggaacctgt gccccatgat gaaacatact gtgaccccgc atctctgttc catgtttcta
     2101 atgattactc attcattcga tattacacaa ggacccttta ccaattccag tttcaagaag
     2161 cactttgtca agcagctaaa catgaaggcc ctctgcacaa atgtgacatc tcaaactcta
     2221 cagaagctgg acagaaactg ttcaatatgc tgaggcttgg aaaatcagaa ccctggaccc
     2281 tagcattgga aaatgttgta ggagcaaaga acatgaatgt aaggccactg ctcaactact
     2341 ttgagccctt atttacctgg ctgaaagacc agaacaaaaa ttcttttgtg ggatggagta
     2401 ccgactggag tccatatgca gaccaaagca tcaaagtgag gataagccta aaatcagctc
     2461 ttggagataa agcatatgaa tggaacgaca atgaaatgta cctgttccga tcatctgttg
     2521 catatgctat gaggcagtac tttttaaaag taaaaaatca gatgattctt tttggggagg
     2581 aggatgtgcg agtggctaat ttgaaaccaa gaatctcctt taatttcttt gtcactgcac
     2641 ctaaaaatgt gtctgatatc attcctagaa ctgaagttga aaaggccatc aggatgtccc
     2701 ggagccgtat caatgatgct ttccgtctga atgacaacag cctagagttt ctggggatac
     2761 agccaacact tggacctcct aaccagcccc ctgtttccat atggctgatt gtttttggag
     2821 ttgtgatggg agtgatagtg gttggcattg tcatcctgat cttcactggg atcagagatc
     2881 ggaagaagaa aaataaagca agaagtggag aaaatcctta tgcctccatc gatattagca
     2941 aaggagaaaa taatccagga ttccaaaaca ctgatgatgt tcagacctcc tttgctagcg
     3001 gcagcggcgc caccaacttc agcctgctga agcaggccgg cgacgtggag gagaaccccg
     3061 gccccgttaa cctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc
     3121 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     3181 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     3241 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     3301 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     3361 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     3421 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     3481 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3541 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3601 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3661 cctggtgcat gacccgcaag cccggtgccg gcggcgggtc cggaggagag ggcagaggaa
     3721 gtctcctaac atgcggtgac gtggaggaga atcctggccc aatgagcgag ctgattaagg
     3781 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
     3841 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     3901 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
     3961 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
     4021 gcttcacatg ggagagagtc accacatacg aggacggggg cgtgctgacc gctacccagg
     4081 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
     4141 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
     4201 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
     4261 ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc
     4321 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
     4381 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
     4441 aactggggca caagcttaat tgagcggccg ctcaacctct ggattacaaa atttgtgaaa
     4501 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4561 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4621 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4681 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4741 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4801 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4861 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4921 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4981 tgccggctct gcggcctctt ccgcgtctac gccttcgccc tcagacgagt cggatctccc
     5041 tttgggccgc ctccccgcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5101 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagtc
     5161 aagatctgct ttttgcctgt actgggtctc tctggttaga ccagagtctc tctggttaga
     5221 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5281 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5341 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtttaa acccgctgat
     5401 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5461 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5521 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5581 gggaggattg ggaagtcaat agcaggcatg ctggggatgc ggtgggctct atgggcggcc
     5641 gttaattaag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     5701 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5761 ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5821 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5881 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5941 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6001 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6061 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6121 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6181 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6241 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6301 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6361 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6421 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6481 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6541 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6601 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6661 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6721 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6781 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6841 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6901 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6961 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7021 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7081 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7141 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7201 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7261 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7321 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7381 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7441 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7501 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7561 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7621 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7681 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7741 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7801 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     7861 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     7921 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7981 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8041 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     8101 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     8161 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     8221 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     8281 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     8341 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     8401 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     8461 cagtgagcgc gcgtaatacg actcactata gggcgaattg actagtacat aacttacggt
     8521 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8581 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8641 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     8701 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     8761 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     8821 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     8881 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     8941 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     9001 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9061 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9121 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9181 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     9241 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     9301 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     9361 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     9421 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     9481 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     9541 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     9601 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     9661 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     9721 ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     9781 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9841 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9901 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9961 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    10021 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    10081 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    10141 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    10201 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    10261 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10321 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10381 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10441 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10501 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10561 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
    10621 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10681 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10741 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtac
//
