LOCUS       sequence-323636-       11282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses soluble, dimeric Fc-His fusion protein for the original 
            SARS-CoV2 Spike S1 region, along with dTomato reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell
            JF, Neelamegham S
  TITLE     Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan 
            elaboration.
  JOURNAL   Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.
  PUBMED    33103998
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-26"; volume: "9. pii: 61552. doi"; pages: " 
            10.7554/eLife.61552"
COMMENT     Sequence Label: S1-Fc
FEATURES             Location/Qualifiers
     source          1..11282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3332..3349
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3365..3421
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3428..4129
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3561..3579)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3829..3848
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4023..4046
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4136..4156
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4157..4174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             4687..4867
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4893..4910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4899..5123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5169..5597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5256..5275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5466..5487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5606..5626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5611..5940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5791..5926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5853..5872
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5988..6035
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6054..6428
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6558..6679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6595..6614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6649..6668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6728..6744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6728..6744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6741..6763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6752..6768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6776..6782,6783..6800,6801..6806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6821..6842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6959..6976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7130..7718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7210..7229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7889..8680,8681..8749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8512..8531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8750..8854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8929..8948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9120..9499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9501..9699
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9717..9897
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9944..10069
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10562..10795
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10980..11024
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caggaattcg attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      601 cgccgccacc atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa
      661 cctgaccaca cggacacagc tgccccctgc ctacaccaac agcttcacaa ggggcgtgta
      721 ctaccccgac aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc
      781 tttcttttct aacgtgacct ggttccacgc tatccacgtg tccggcacca acggaacaaa
      841 gaggttcgac aacccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa
      901 gtctaacatc atcagaggct ggatctttgg aaccacactg gacagcaaga cacagtccct
      961 gctgatcgtg aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa
     1021 cgatccattc ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt
     1081 tcgcgtgtac tcctctgcca acaactgtac atttgagtac gtgtcccagc ccttcctgat
     1141 ggacctggag ggcaagcagg gaaacttcaa gaacctgcgg gagttcgtgt ttaagaacat
     1201 cgatggctac tttaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc
     1261 acagggcttc tctgccctgg agccactggt ggatctgccc atcggaatca acatcaccag
     1321 gtttcagaca ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg
     1381 atggaccgct ggagctgctg cctactacgt gggctacctg cagccccgga ccttcctgct
     1441 gaagtacaac gagaacggaa ccatcacaga cgctgtggat tgcgccctgg accccctgtc
     1501 tgagaccaag tgtacactga agagctttac cgtggagaag ggcatctacc agacaagcaa
     1561 cttccgggtg cagcctaccg agtccatcgt gcgctttccc aacatcacaa acctgtgccc
     1621 ttttggagag gtgttcaacg ctacccgctt cgcctccgtg tacgcttgga accggaagcg
     1681 catctccaac tgcgtggccg actactctgt gctgtacaac agcgccagct tcagcacctt
     1741 caagtgctac ggcgtgagcc caacaaagct gaacgacctg tgctttacca acgtgtacgc
     1801 tgattccttc gtgatcaggg gagacgaggt gcgccagatc gctcccggcc agacaggaaa
     1861 gatcgctgac tacaactaca agctgcctga cgatttcacc ggctgcgtga tcgcctggaa
     1921 ctctaacaac ctggatagca aagtgggcgg aaactacaac tacctgtaca ggctgtttag
     1981 aaagtctaac ctgaagccat tcgagcggga catctccaca gagatctacc aggctggctc
     2041 taccccatgc aacggagtgg agggcttcaa ctgttacttc cctctgcaga gctacggatt
     2101 ccagccaaca aacggcgtgg gataccagcc ctaccgcgtg gtggtgctgt cttttgagct
     2161 gctgcacgct cctgctacag tgtgcggacc aaagaagagc accaacctgg tgaagaacaa
     2221 gtgcgtgaac ttcaacttta acggactgac cggcacagga gtgctgaccg agtctaacaa
     2281 gaagttcctg ccttttcagc agttcggccg ggacatcgcc gataccacag acgctgtgcg
     2341 cgaccctcag accctggaga tcctggatat cacaccatgc tccttcggcg gagtgtctgt
     2401 gatcacacca ggaaccaaca caagcaacca ggtggccgtg ctgtaccagg acgtgaactg
     2461 taccgaggtg cccgtggcta tccacgccga tcagctgacc cctacatgga gggtgtactc
     2521 taccggcagc aacgtgttcc agacaagagc cggctgtctg atcggagctg agcacgtgaa
     2581 caacagctac gagtgcgaca tccctatcgg cgccggaatc tgtgcttcct accagaccca
     2641 gacaaactcc ccaaggtcta gaacatgccc accgtgccca gcacctgaac tcgcgggggc
     2701 accgtcagtc ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc
     2761 tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg
     2821 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa
     2881 cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa
     2941 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
     3001 caaagccaaa gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggatga
     3061 gctgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
     3121 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
     3181 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
     3241 gcagcagggg aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac
     3301 gcagaagagc ctctccctgt ctccgggtaa acaccatcat catcatcatt tcgaaggcag
     3361 cggcgccacc aacttcagcc tgctgaagca ggccggcgac gtggaggaga accccggccc
     3421 cgttaacatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
     3481 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3541 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     3601 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     3661 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3721 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     3781 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     3841 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     3901 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     3961 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     4021 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     4081 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt aagtcgaaaa
     4141 cctgtacttc cagggccatc accatcacca tcactgactc gagacctaga aaaacatgga
     4201 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4261 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4321 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4381 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4441 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     4501 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     4561 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     4621 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     4681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4861 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4921 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4981 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5041 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5101 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5161 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5221 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5281 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5341 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5401 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5461 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5521 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5581 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5761 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     5881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5941 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     6001 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     6061 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     6121 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     6181 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     6241 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     6301 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     6361 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     6421 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6481 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6541 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6601 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6661 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6721 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6901 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8821 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8881 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     8941 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9001 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9061 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     9121 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     9181 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     9241 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     9301 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     9361 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     9421 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     9481 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     9541 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     9601 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     9661 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
     9721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     9901 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     9961 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
    10021 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
    10081 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
    10141 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
    10201 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
    10261 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
    10321 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
    10381 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
    10441 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    10501 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    10561 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    10621 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    10681 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    10741 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    10801 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    10861 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    10921 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    10981 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    11041 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    11101 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    11161 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
    11221 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcccccgggc
    11281 tg
//
