LOCUS       sequence-323641-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses soluble , dimeric Fc-His fusion protein for the human ACE2
            extracellular domain, along with dTomato reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell
            JF, Neelamegham S
  TITLE     Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan 
            elaboration.
  JOURNAL   Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.
  PUBMED    33103998
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-26"; volume: "9. pii: 61552. doi"; pages: " 
            10.7554/eLife.61552"
COMMENT     Sequence Label: ACE2-Fc
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2463..2766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2767..2970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5924..5941
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5957..6013
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6020..6721
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6153..6171)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6421..6440
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6615..6638
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6728..6748
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6749..6766
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             7279..7459
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7485..7502)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7491..7715
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7761..8189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7848..7867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8058..8079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8198..8218)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8203..8532
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8383..8518
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8445..8464
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8580..8627
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8646..9020
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9150..9271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9187..9206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9241..9260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9320..9336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9320..9336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9333..9355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9344..9360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9368..9374,9375..9392,9393..9398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9413..9434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9551..9568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9722..10310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9802..9821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10481..11272,11273..11341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11104..11123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11342..11446)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2461 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2521 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2581 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2641 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2701 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2761 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2821 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2881 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2941 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     3001 cgccaccatg attcctgcca gatttgccgg ggtgctgctt gctctggccc tcattttgcc
     3061 agggaccctt tgtaccggtc agtccaccat tgaggaacag gccaagacat ttttggacaa
     3121 gtttaaccac gaagccgaag acctgttcta tcaaagttca cttgcttctt ggaattataa
     3181 caccaatatt actgaagaga atgtccaaaa catgaataat gctggggaca aatggtctgc
     3241 ctttttaaag gaacagtcca cacttgccca aatgtatcca ctacaagaaa ttcagaatct
     3301 cacagtcaag cttcagctgc aggctcttca gcaaaatggg tcttcagtgc tctcagaaga
     3361 caagagcaaa cggttgaaca caattctaaa tacaatgagc accatctaca gtactggaaa
     3421 agtttgtaac ccagataatc cacaagaatg cttattactt gaaccaggtt tgaatgaaat
     3481 aatggcaaac agtttagact acaatgagag gctctgggct tgggaaagct ggagatctga
     3541 ggtcggcaag cagctgaggc cattatatga agagtatgtg gtcttgaaaa atgagatggc
     3601 aagagcaaat cattatgagg actatgggga ttattggaga ggagactatg aagtaaatgg
     3661 ggtagatggc tatgactaca gccgcggcca gttgattgaa gatgtggaac atacctttga
     3721 agagattaaa ccattatatg aacatcttca tgcctatgtg agggcaaagt tgatgaatgc
     3781 ctatccttcc tatatcagtc caattggatg cctccctgct catttgcttg gtgatatgtg
     3841 gggtagattt tggacaaatc tgtactcttt gacagttccc tttggacaga aaccaaacat
     3901 agatgttact gatgcaatgg tggaccaggc ctgggatgca cagagaatat tcaaggaggc
     3961 cgagaagttc tttgtatctg ttggtcttcc taatatgact caaggattct gggaaaattc
     4021 catgctaacg gacccaggaa atgttcagaa agcagtctgc catcccacag cttgggacct
     4081 ggggaagggc gacttcagga tccttatgtg cacaaaggtg acaatggacg acttcctgac
     4141 agctcatcat gagatggggc atatccagta tgatatggca tatgctgcac aaccttttct
     4201 gctaagaaat ggagctaatg aaggattcca tgaagctgtt ggggaaatca tgtcactttc
     4261 tgcagccaca cctaagcatt taaaatccat tggtcttctg tcacccgatt ttcaagaaga
     4321 caatgaaaca gaaataaact tcctgctcaa acaagcactc acgattgttg ggactctgcc
     4381 atttacttac atgttagaga agtggaggtg gatggtcttt aaaggggaaa ttcccaaaga
     4441 ccagtggatg aaaaagtggt gggagatgaa gcgagagata gttggggtgg tggaacctgt
     4501 gccccatgat gaaacatact gtgaccccgc atctctgttc catgtttcta atgattactc
     4561 attcattcga tattacacaa ggacccttta ccaattccag tttcaagaag cactttgtca
     4621 agcagctaaa catgaaggcc ctctgcacaa atgtgacatc tcaaactcta cagaagctgg
     4681 acagaaactg ttcaatatgc tgaggcttgg aaaatcagaa ccctggaccc tagcattgga
     4741 aaatgttgta ggagcaaaga acatgaatgt aaggccactg ctcaactact ttgagccctt
     4801 atttacctgg ctgaaagacc agaacaagaa ttcttttgtg ggatggagta ccgactggag
     4861 tccatatgca gaccaaagca tcaaagtgag gataagccta aaatcagctc ttggagataa
     4921 agcatatgaa tggaacgaca atgaaatgta cctgttccga tcatctgttg catatgctat
     4981 gaggcagtac tttttaaaag taaaaaatca gatgattctt tttggggagg aggatgtgcg
     5041 agtggctaat ttgaaaccaa gaatctcctt taatttcttt gtcactgcac ctaaaaatgt
     5101 gtctgatatc attcctagaa ctgaagttga aaaggccatc aggatgtccc ggagccgtat
     5161 caatgatgct ttccgtctga atgacaacag cctagagttt ctggggatac agccaacact
     5221 tggacctcct aaccagcccc ctgtttcctc tagaacatgc ccaccgtgcc cagcacctga
     5281 actcgcgggg gcaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat
     5341 ctcccggacc cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt
     5401 caagttcaac tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga
     5461 ggagcagtac aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg
     5521 gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga
     5581 gaaaaccatc tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc
     5641 atcccgggat gagctgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta
     5701 tcccagcgac atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac
     5761 cacgcctccc gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga
     5821 caagagcagg tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca
     5881 caaccactac acgcagaaga gcctctccct gtctccgggt aaacaccatc atcatcatca
     5941 tttcgaaggc agcggcgcca ccaacttcag cctgctgaag caggccggcg acgtggagga
     6001 gaaccccggc cccgttaaca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg
     6061 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     6121 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     6181 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     6241 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     6301 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     6361 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     6421 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     6481 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     6541 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     6601 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     6661 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
     6721 gtaagtcgaa aacctgtact tccagggcca tcaccatcac catcactgac tcgagaccta
     6781 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6841 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6901 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6961 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     7021 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     7081 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     7141 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     7201 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     7261 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7321 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7381 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7441 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     7501 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7561 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7621 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7681 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7741 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7801 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7861 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7921 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7981 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8041 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8101 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8161 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     8221 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8281 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8341 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8461 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8521 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     8581 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     8641 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     8701 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     8761 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     8821 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     8881 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     8941 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     9001 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     9061 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9121 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9301 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11461 aagtgccacc tgacgtc
//
