LOCUS       sequence-323643-        9331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a
            C-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae 
            LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE
  TITLE     An essential receptor for adeno-associated virus infection.
  JOURNAL   Nature. 2016 Feb 4;530(7588):108-12. doi: 10.1038/nature16465. Epub 
            2016 Jan 27.
  PUBMED    26814968
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16465"; journalName: "Nature"; date: "2016-02-4- 4"; 
            volume: "530"; issue: "7588"; pages: "108-12"
COMMENT     Sequence Label: pLenti-CMV-Puro-miniAAVR-FLAG
FEATURES             Location/Qualifiers
     source          1..9331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4528..4551
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4581..5169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4634..4654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5052..5063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5183..5682
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5218..5241)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5615..5634
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5703..6302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5703..5722)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6199..6219
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6440..6673
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6745..6866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6782..6801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6836..6855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6906..7041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7061..7080)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7062..7080)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7090..7107)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7090..7106)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7099..7121)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7248..7703
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7335..7354)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7545..7566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7729..7833
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7834..7902,7903..8694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8052..8071)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8865..9331,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatac catggagaag aggctgggag tcaagccaaa tcctgcttcc
     3181 tggattttat caggatatta ttggcagaca tctgcgaagt ggttgagaag cctgtacctg
     3241 ttttatactt gcttttgctt cagcgttctg tggttgtcaa cagatgccag tgaggtatct
     3301 gctggagaga gtgtccagat aaccctgcct aagaatgaag ttcaattaaa tgcatatgtt
     3361 ctccaagaac cacctaaagg agaaacctac acctacgact ggcagctgat tactcatcct
     3421 agagactaca gtggagaaat ggaagggaaa cattcccaga tcctcaaact atcgaagctc
     3481 actccaggcc tgtatgaatt caaagtgatt gtagagggtc aaaatgccca tggggaaggc
     3541 tatgtgaacg tgacagtcaa gccagagccc cgtaagaatc ggccccccat tgctattgtg
     3601 tcacctcagt tccaggagat ctctttgcca accacttcta cagtcattga tggcagtcaa
     3661 agcactgatg atgataaaat cgttcagtac cattgggaag aacttaaggg gcctctaaga
     3721 gaagagaaga tttctgaaga tacagccata ttaaaactaa gtaaactcgt ccctgggaac
     3781 tacactttca gcttgactgt agtagactct gatggagcta ccaactctac tactgcaaac
     3841 ctgacagtga acaaagctgt ggattacccc cctgtggcca acgcaggccc caaccaagtg
     3901 atcaccctgc cccaaaactc catcaccctc tttgggaacc agagcactga tgatcatggc
     3961 atcaccagct atgagtggtc actcagccca agcagcaaag ggaaagtggt ggagatgcag
     4021 ggtgttagaa caccaacctt acagctctct gcgatgcaag aaggagacta cacttaccag
     4081 ctcacagtga ctgacacaat aggacagcag gccactgctc aagtgactgt tattgtgcaa
     4141 cctgaaaaca ataagtgtga gtggagcgtg ttatatgtta tcattgctac ctttgtcatt
     4201 gttgttgcct tgggaatcct gtcttggact gtgatctgtt gttgtaagag gcaaaaagga
     4261 aaacccaaga ggaaaagcaa gtacaagatc ctggatgcca cggatcagga aagcctggag
     4321 ctgaagccaa cctcccgagc aggcatcaaa cagaaaggcc ttttgctaag tagcagcctg
     4381 atgcactccg agtcagagct ggacagcgat gatgccatct ttacatggcc agaccgagag
     4441 aagggcaaac tcctgcatgg tcagaatggc tctgtaccca acgggcagac ccctctgaag
     4501 gccaggagcc cgcgggagga gatcctggat tacaaggatg acgacgataa gtaaatccag
     4561 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4981 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5161 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     5221 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     5281 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     5341 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     5401 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     5461 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     5521 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     5581 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     5641 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     5701 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5761 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5821 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5881 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5941 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6001 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6061 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6121 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6181 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6241 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6301 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc
     6361 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6421 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
     6481 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6541 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6601 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6661 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6721 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6841 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6901 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6961 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7021 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     7081 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7201 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     7261 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7321 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7381 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7441 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7501 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7561 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7621 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7681 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7801 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7861 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7921 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7981 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8041 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8101 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8161 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8221 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8281 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8341 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8401 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8461 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8521 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8581 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8641 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8701 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8761 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8821 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8881 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8941 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9001 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9061 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9121 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9181 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9241 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9301 tatccggtaa gcggcagggt cggaacagga g
//
