LOCUS       sequence-323652-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for
            pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pTwist-WIV1-CoV Delta-18
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      252..388
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    540..611
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(616..662)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    680..728
                     /note="synthetic polyadenylation signal"
     enhancer        809..1112
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1114..1316
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1267..1287
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1450..1925
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5771..6359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5824..5844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6242..6253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6412..6533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6423..6442)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6477..6496
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6648..6694
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6724..6860
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6905..6976
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        7021..7217
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7068..7203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7231..8025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7285..7304)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7895..7914
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8036..8260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8456..9044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8536..8555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9790..9858,9859..10411,1..239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10008..10027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
       61 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
      121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
      181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
      241 cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact
      301 cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt
      361 gtcctcctta ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc
      421 tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg
      481 gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac
      541 atcttggacc attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc
      601 agctacctct caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
      661 tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg
      721 attttattat ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat
      781 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      841 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      901 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      961 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1021 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1081 acatctacgt attagtcatc gctattacca tgctgatgcg gttttggcag tacatcaatg
     1141 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1201 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1261 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     1321 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     1381 accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc ggatcctgag
     1441 aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta
     1501 agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa
     1561 ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt
     1621 atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc
     1681 ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat
     1741 ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct
     1801 aatagcagct acaatccagc taccattctg cttttatttt atggttggga taaggctgga
     1861 ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc
     1921 cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaaggcggcc
     1981 gccatgaaat tgctggtact ggtctttgcg acactcgtgt caagctatac catcgaaaag
     2041 tgtcttgact tcgatgatcg gacacccccg gccaataccc agttcctgtc aagccaccga
     2101 ggggtctatt acccggacga tattttcaga agcaatgtac tgcatttggt acaggaccac
     2161 tttctccctt ttgattccaa tgttactagg tttatcactt ttgggcttaa tttcgacaat
     2221 cctataattc ctttcaaaga tggcatctac ttcgctgcta ctgaaaagtc caacgttatt
     2281 agaggctggg tgtttggatc aacaatgaat aacaagtctc aatccgtcat cataatgaac
     2341 aatagtacca accttgtaat ccgggcttgc aattttgaat tgtgcgacaa tcctttcttt
     2401 gttgtgctca agagcaacaa tacacaaatt cctagctaca tctttaataa tgccttcaac
     2461 tgtactttcg agtatgtgag caaagacttt aatcttgacc tcggcgagaa gcccggaaac
     2521 ttcaaagatt tgcgcgaatt tgtgtttaga aacaaagacg gcttcctgca cgtctactcc
     2581 ggctatcaac caatcagcgc ggccagcggg ttgccaactg gctttaatgc gctcaagcct
     2641 attttcaagc tcccgctggg aatcaacatt actaatttcc gcaccctgct caccgctttt
     2701 ccccctcgcc ctgactattg gggcacaagc gccgcagctt attttgtcgg ttacttgaaa
     2761 cccactacct tcatgttgaa gtacgacgaa aacggcacta ttacagatgc agtagactgt
     2821 agccaaaatc cactggcgga gctgaaatgc agtgtcaagt cattcgagat cgacaaaggc
     2881 atctaccaga cttcaaattt tcgcgtcgca ccatccaaag aagtagtgcg ctttccaaat
     2941 atcaccaatc tgtgtccttt tggtgaagtg tttaacgcca ccacattccc cagtgtttac
     3001 gcctgggaga gaaagaggat cagcaactgt gtggctgact attcagtgct gtataatagc
     3061 acctctttta gtacattcaa atgttacggg gtgtcagcca ccaagctcaa cgacctgtgt
     3121 tttagcaacg tatacgcaga tagtttcgtc gtcaaaggag atgacgttag acagatcgct
     3181 cccggccaaa caggggttat tgctgactac aactataagc tgcccgatga ttttactggc
     3241 tgtgtgctgg cttggaacac acgcaacata gacgccacgc agacgggcaa ttacaactac
     3301 aagtaccgtt ccctgagaca cggcaaactg agaccctttg aaagggacat ctccaatgtg
     3361 cctttttctc ctgacggcaa gccctgtacc ccacccgctt ttaattgtta ctggccgctg
     3421 aatgattatg gtttctatat aactaacggc ataggctatc agccataccg ggttgttgtg
     3481 ctcagtttcg agttgcttaa cgctccagca actgtctgtg gcccaaaact cagtaccgat
     3541 ttgataaaga atcaatgcgt gaactttaat tttaacggat tgacagggac aggcgttttg
     3601 actcctagtt ccaaacggtt ccaacccttt caacaattcg ggcgcgatgt ctctgatttc
     3661 accgattctg taagagatcc taagacctct gaaatacttg acatcagtcc ttgttctttc
     3721 ggcggtgtat ctgtaatcac accgggcact aacacgagca gtgaagtagc cgtactgtat
     3781 caggatgtta attgcactga cgtcccggta gcaatacacg cagatcaact gacgccatct
     3841 tggcgagttc actctaccgg caataacgtc tttcaaactc aagcaggatg cttgattggg
     3901 gcggagcacg tagataccag ttatgagtgc gatattccga taggcgcggg catctgtgcc
     3961 agttatcaca ccgtcagtag tctccggtct actagccaga aaagtattgt cgcatacaca
     4021 atgtcactcg gtgccgatag ttctatcgcc tactccaaca acaccattgc aatccccact
     4081 aatttctcca tctccatcac caccgaagtg atgcctgtca gcatggctaa aaccagcgtt
     4141 gactgcaata tgtatatttg cggcgattca acggagtgcg cgaatttgct ccttcaatat
     4201 ggctcatttt gcacacaact gaaccgggct ctctccggaa tcgcggtgga acaagacagg
     4261 aacactcgtg aagtgttcgc gcaagtcaag caaatgtata agactccgac ccttaaagat
     4321 ttcggcgggt ttaatttctc ccaaattctc ccggacccct tgaagcctac caagcgcagc
     4381 tttatcgaag atttgttgtt caacaaagtt acccttgccg acgccggttt tatgaagcaa
     4441 tacggggaat gtcttggaga tataaatgcg cgtgatctta tttgtgccca aaaattcaac
     4501 ggcttgacag tgctgccccc actcctgacg gacgatatga tagccgcata cactgcggcg
     4561 ttggtgagcg gcaccgccac cgctggctgg actttcgggg caggagccgc tttgcaaatt
     4621 ccctttgcga tgcaaatggc gtataggttt aacgggatag gcgtaacaca aaatgttctc
     4681 tacgaaaatc aaaagcaaat cgctaatcaa tttaacaagg ccatttccca aattcaggag
     4741 agtctcacaa caacatctac tgctctggga aaactgcaag acgttgtgaa tcaaaacgct
     4801 caagcactca atacactcgt taagcaactg tcctctaact tcggggccat atcttctgtg
     4861 ctgaatgata ttctgtcacg cctcgataaa gttgaggctg aagtccagat agacagactg
     4921 attacgggca ggctgcaaag cctgcaaacc tatgtgacgc agcagctcat tcgagctgct
     4981 gaaattcgcg cttcagcgaa cctcgcagcc accaaaatgt ctgaatgtgt tttggggcaa
     5041 agcaaacggg tcgatttctg tggtaaagga taccatttga tgtcttttcc ccaagcagct
     5101 ccacacggcg ttgtctttct gcatgtgacc tatgtcccca gccaagaacg gaatttcaca
     5161 acggctcccg ccatctgtca cgaagggaag gcgtattttc ctcgggaagg ggtgtttgtt
     5221 ttcaatggga cctcatggtt tattacgcag cgcaatttct ttagccccca aataataacc
     5281 acagacaata catttgtcag tggtagttgc gatgtagtca ttggaataat caataatacg
     5341 gtgtacgatc ctttgcagcc agaattggat agcttcaaag aagaacttga caaatacttt
     5401 aagaaccaca caagtccaga cgtggacttg ggagatataa gcgggataaa cgcgagcgtt
     5461 gtcaatattc aaaaggaaat cgacaggctg aatgaagtag ctaagaatct gaatgaatcc
     5521 ctcattgatt tgcaagaact cgggaagtat gagcaatata tcaagtggcc gtggtatgtt
     5581 tggctgggtt ttatagccgg tctgattgca atcgtcatgg tgacaattct gttgtgttgc
     5641 atgacttcat gctgtagttg cctcaaaggc gcatgtagct gtggcagttg ttgtaaataa
     5701 aggatcctct agaagttgtc tcctcctgca ctgactgact gatacaatcg atttctggat
     5761 ccgcaggcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5821 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5881 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5941 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6001 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6061 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6121 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6181 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6241 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6301 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6361 tgtctagctt gactgactga gatacagcgt accttcagct cacagacatg ataagataca
     6421 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6481 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     6541 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     6601 agtaaaacct ctacaaatgt ggtattggcc catctctatc ggtatcgtag cataacccct
     6661 tggggcctct aaacgggtct tgaggggttt tttgtgcccc tcgggccgga ttgctatcta
     6721 ccggcattgg cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata
     6781 tgccagattc agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct
     6841 taccagaaat ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc
     6901 aagacattcc tttaatggtc ttttctggac accactaggg gtcagaagta gttcatcaaa
     6961 ctttcttccc tccctaatct cattggttac cttgggctat cgaaacttaa ttaagcgatc
     7021 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7081 ctccgcccag ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag
     7141 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7201 gcctaggctt ttgcaaagga ggtagccaac atgattgaac aagatggatt gcacgcaggt
     7261 tctcccgccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7321 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7381 accgacctgt ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg
     7441 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7501 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7561 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7621 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7681 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7741 ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccacggcgat
     7801 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7861 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7921 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7981 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctagtatgt aagccctgtg
     8041 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8101 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8161 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8221 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg ttaattaacc agtcaagtca
     8281 gctacttggc gagatcgact tgtctgggtt tcgactacgc tcagaattgc gtcagtcaag
     8341 ttcgatctgg tccttgctat tgcacccgtt ctccgattac gagtttcatt taaatcatgt
     8401 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8461 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8521 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8581 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8641 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8701 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8761 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8821 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8881 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8941 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9001 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9061 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9121 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9181 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9241 ctatctcagc gatctgtcta tttcgttcat ccatagttgc atttaaattt ccgaactctc
     9301 caaggccctc gtcggaaaat cttcaaacct ttcgtccgat ccatcttgca ggctacctct
     9361 cgaacgaact atcgcaagtc tcttggccgg ccttgcgcct tggctattgc ttggcagcgc
     9421 ctatcgccag gtattactcc aatcccgaat atccgagatc gggatcaccc gagagaagtt
     9481 caacctacat cctcaatccc gatctatccg agatccgagg aatatcgaaa tcggggcgcg
     9541 cctggtgtac cgagaacgat cctctcagtg cgagtctcga cgatccatat cgttgcttgg
     9601 cagtcagcca gtcggaatcc agcttgggac ccaggaagtc caatcgtcag atattgtact
     9661 caagcctggt cacggcagcg taccgatctg tttaaaccta gatattgata gtctgatcgg
     9721 tcaacgtata atcgagtcct agcttttgca aacatctatc aagagacagg atcagcagga
     9781 ggctttcgca tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9841 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9901 ttgggtgcgc gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9961 tttcgccccg aagaacgctt tccaatgatg agcactttta aagttctgct atgtggcgcg
    10021 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    10081 aatgacttgg ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta
    10141 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    10201 acaacgattg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    10261 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    10321 accacgatgc ctgtagcaat ggcaacaacc ttgcgtaaac tattaactgg cgaactactt
    10381 actctagctt cccggcaaca gttgatagac t
//
