LOCUS       sequence-323653-        8336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids 
            for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pHDM-SARS-CoV-2 Delta-18
FEATURES             Location/Qualifiers
     source          1..8336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4214..4230)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4215..4231)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4324..4718
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5052..5070)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5138..5242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5243..5311,5312..6103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5461..5480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6274..6862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6763..6782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7526..7905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7906..8109
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8060..8080
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8106..8130
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(8223..8336,1..362)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 tgcatttgta attttaaaaa atgctttctt cttttaatat acttttttgt ttatcttatt
       61 tctaatactt tccctaatct ctttctttca gggcaataat gatacaatgt atcatgcctc
      121 tttgcaccat tctaaagaat aacagtgata atttctgggt taaggcaata gcaatatttc
      181 tgcatataaa tatttctgca tataaattgt aactgatgta agaggtttca tattgctaat
      241 agcagctaca atccagctac cattctgctt ttattttatg gttgggataa ggctggatta
      301 ttctgagtcc aagctaggcc cttttgctaa tcatgttcat acctcttatc ttcctcccac
      361 agctcctggg caacgtgctg gtctgtgtgc tggcccatca ctttggcaaa gaattccgcg
      421 ggcggccgcc atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa
      481 cctgaccacc agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta
      541 ctaccccgac aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc
      601 tttcttcagc aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa
      661 gagattcgac aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa
      721 gtccaacatc atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct
      781 gctgatcgtg aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa
      841 cgaccccttc ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt
      901 ccgggtgtac agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat
      961 ggacctggaa ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat
     1021 cgacggctac ttcaaaatct acagcaagca cacccctatc aacctcgtgc gggatctgcc
     1081 tcagggcttc tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg
     1141 gtttcagaca ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg
     1201 atggacagct ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct
     1261 gaagtacaac gagaacggca ccatcaccga cgccgtggat tgtgctctgg accctctgag
     1321 cgagacaaag tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa
     1381 cttccgggtg cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc
     1441 cttcggcgag gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg
     1501 gatcagcaat tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt
     1561 caagtgctac ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc
     1621 cgacagcttc gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa
     1681 gatcgccgac tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa
     1741 cagcaacaac ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg
     1801 gaagtccaat ctgaagccct tcgagcggga catcagcacc gaaatctatc aggccggcag
     1861 caccccttgc aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt
     1921 tcagcccaca aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact
     1981 gctgcatgcc cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa
     2041 atgcgtgaac ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa
     2101 gaagttcctg ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag
     2161 agatccccag acactggaaa tcctggacat caccccatgc agcttcggcg gagtgtctgt
     2221 gatcacccct ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg
     2281 taccgaagtg cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc
     2341 caccggcagc aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa
     2401 caatagctac gagtgcgaca tccccatcgg cgctggcatc tgtgccagct accagacaca
     2461 gacaaacagc cccagacggg ctagaagcgt ggccagccag agcatcattg cctacacaat
     2521 gtctctgggc gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa
     2581 cttcaccatc agcgtgacca ccgagatcct gcctgtgtcc atgaccaaga ccagcgtgga
     2641 ctgcaccatg tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg
     2701 cagcttctgc acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa
     2761 cacccaagag gtgttcgccc aagtgaagca aatctacaag acccctccta tcaaggactt
     2821 cggcggcttc aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt
     2881 catcgaggac ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta
     2941 tggcgattgt ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg
     3001 actgacagtg ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct
     3061 gctggccggc acaatcacaa gcggctggac atttggagct ggcgccgctc tgcagatccc
     3121 ctttgctatg cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta
     3181 cgagaaccag aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag
     3241 cctgagcagc acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca
     3301 ggcactgaac accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct
     3361 gaacgatatc ctgagcagac tggacaaggt ggaagccgag gtgcagatcg acagactgat
     3421 caccggaagg ctgcagtccc tgcagaccta cgttacccag cagctgatca gagccgccga
     3481 gattagagcc tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag
     3541 caagagagtg gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc
     3601 tcacggcgtg gtgtttctgc acgtgacata cgtgcccgct caagagaaga atttcaccac
     3661 cgctccagcc atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc
     3721 caacggcacc cattggttcg tgacccagcg gaacttctac gagccccaga tcatcaccac
     3781 cgacaacacc ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt
     3841 gtacgaccct ctgcagcccg agctggacag cttcaaagag gaactggata agtactttaa
     3901 gaaccacaca agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt
     3961 gaacatccag aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct
     4021 gatcgacctg caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg
     4081 gctgggcttt atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat
     4141 gaccagctgc tgtagctgcc tgaagggctg ttgctcttgc ggctcttgct gcaaataaag
     4201 gatccaagct tatcgatacc gtcgacctcg agggcccaga tctaattcac cccaccagtg
     4261 caggctgcct atcagaaagt ggtggctggt gtggctaatg ccctggccca caagtatcac
     4321 taagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc taagtccaac
     4381 tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct aataaaaaac
     4441 atttattttc attgcaatga tgtatttaaa ttatttctga atattttact aaaaagggaa
     4501 tgtgggaggt cagtgcattt aaaacataaa gaaatgaaga gctagttcaa accttgggaa
     4561 aatacactat atcttaaact ccatgaaaga aggtgaggct gcaaacagct aatgcacatt
     4621 ggcaacagcc cctgatgcct atgccttatt catccctcag aaaaggattc aagtagaggc
     4681 ttgatttgga ggttaaagtt ttgctatgct gtattttaca ttacttattg ttttagctgt
     4741 cctcatgaat gtcttttcac tacccatttg cttatcctgc atctctcagc cttgactcca
     4801 ctcagttctc ttgcttagag ataccacctt tcccctgaag tgttccttcc atgttttacg
     4861 gcgagatggt ttctcctcgc ctggccactc agccttagtt gtctctgttg tcttatagag
     4921 gtctacttga agaaggaaaa acagggggca tggtttgact gtcctgtgag cccttcttcc
     4981 ctgcctcccc cactcacagt gacccggaat ccctcgacat ggcagtctag atcattcttg
     5041 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5101 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5161 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5221 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5281 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5341 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5401 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5461 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5521 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5581 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5641 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5701 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5761 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5821 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5881 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5941 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6001 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6061 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6121 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6181 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6541 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6601 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6661 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6721 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6781 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6841 aggggggcgg agcctatgga aaaacgccag caacggagat gcgccgcgtg cggctgctgg
     6901 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
     6961 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc
     7021 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac
     7081 aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc
     7141 ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag
     7201 cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     7261 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     7321 tcgcgtcggg gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     7381 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     7441 catgagcttg gcccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     7501 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     7561 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     7621 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     7681 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     7741 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     7801 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     7861 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     7921 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     7981 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     8041 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     8101 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     8161 ctccatagaa gacaccggga ccgatccagc ctcccctcga agctgatcct gagaacttca
     8221 gggtgagtct atgggaccct tgatgttttc tttccccttc ttttctatgg ttaagttcat
     8281 gtcataggaa ggggagaagt aacagggtac acatattgac caaatcaggg taattt
//
