LOCUS       sequence-323654-        8910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes codon-optimized HIV gagpol for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pHDM-Hgpm2
FEATURES             Location/Qualifiers
     source          1..8910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          114..589
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(5015..5031)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5016..5032)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5125..5519
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5853..5871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5939..6043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6044..6112,6113..6904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6262..6281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7075..7663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7564..7583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8327..8706
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8707..8910
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8861..8881
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     join(8907..8910,1..21)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
       61 agacaccggg accgatccag cctcccctcg aagctgatcc tgagaacttc agggtgagtc
      121 tatgggaccc ttgatgtttt ctttcccctt cttttctatg gttaagttca tgtcatagga
      181 aggggagaag taacagggta cacatattga ccaaatcagg gtaattttgc atttgtaatt
      241 ttaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
      301 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
      361 aaagaataac agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat
      421 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
      481 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
      541 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
      601 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa ttctagactg ccatgggcgc
      661 ccgcgcctcc gtgctgtccg gcggcgagct ggacaagtgg gagaagatcc gcctgcgccc
      721 cggcggcaag aagcagtaca agctgaagca catcgtgtgg gcctcccgcg agctggagcg
      781 cttcgccgtg aaccccggcc tgctggagac ctccgagggc tgccgccaga tcctgggcca
      841 gctgcagccc tccctgcaaa ccggctccga ggagctgcgc tccctgtaca acaccatcgc
      901 cgtgctgtac tgcgtgcacc agcgcatcga cgtgaaggac accaaggagg ccctggacaa
      961 gatcgaggag gagcagaaca agtccaagaa gaaggcccag caggccgccg ccgacaccgg
     1021 caacaactcc caggtgtccc agaactaccc catcgtgcag aacctgcagg gccagatggt
     1081 gcaccaggcc atctcccccc gcaccctgaa cgcctgggtg aaggtggtgg aggagaaggc
     1141 cttctccccc gaagtcatcc ccatgttctc cgccctgtcc gagggcgcca ccccccagga
     1201 cctgaacacc atgctgaaca ccgtgggcgg ccaccaggcc gccatgcaga tgctgaagga
     1261 gaccatcaac gaggaggccg ccgagtggga ccgcctgcac cccgtgcacg ccggccccat
     1321 cgcccccggc cagatgcgcg agccccgcgg ctccgacatc gccggcacca cctccaccct
     1381 gcaagagcag atcggctgga tgacccacaa cccccccatc cccgtgggcg agatctacaa
     1441 gcgctggatc atcctgggcc tgaacaagat cgtgcgcatg tactccccca cctccatcct
     1501 ggacatccgc cagggcccca aggagccctt ccgcgactac gtggaccgct tctacaagac
     1561 cctgcgcgcc gagcaggcct cccaggaggt aaagaactgg atgaccgaga ccctgctggt
     1621 gcagaacgcc aaccccgact gcaagaccat cctgaaggcc ctgggccccg gcgccaccct
     1681 ggaggagatg atgaccgcct gccagggcgt gggcggcccc ggccacaagg cccgcgtgct
     1741 ggccgaggcc atgtcccaag tcaccaaccc cgccaccatc atgatccaga agggcaactt
     1801 ccgcaaccag cgcaagaccg tgaagtgctt caactgcggc aaggagggcc acatcgccaa
     1861 gaactgccgc gccccccgca agaagggctg ctggaagtgc ggcaaggagg gccaccagat
     1921 gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt cccacaaggg
     1981 aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag aagagagctt
     2041 caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag acaaggaact
     2101 gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac aataaagatc
     2161 ggtggccagc tgaaggaggc cctgctggac accggcgccg acgacaccgt gctggaggag
     2221 atgaacctgc ccggccgctg gaagcccaag atgatcggcg gcatcggcgg cttcatcaaa
     2281 gtccgccagt acgaccagat cctgatcgag atctgcggcc acaaggccat cggcaccgtg
     2341 ctggtgggcc ccacccccgt gaacatcatc ggccgcaacc tgctgaccca gatcggctgc
     2401 accctgaact tccccatctc ccccatcgag accgtgcccg tgaagctgaa gcccggcatg
     2461 gacggcccca aagtcaagca gtggcccctg accgaggaga agatcaaggc cctggtggag
     2521 atctgcaccg agatggagaa ggagggcaag atctccaaga tcggccccga gaacccctac
     2581 aacacccccg tgttcgccat caagaagaag gactccacca agtggcgcaa gctggtggac
     2641 ttccgcgagc tgaacaagcg cacccaggac ttctgggagg tgcagctggg catcccccac
     2701 cccgccggcc tgaagcagaa gaagtccgtg accgtgctgg acgtgggcga cgcctacttc
     2761 tccgtgcccc tggacaagga cttccgcaag tacaccgcct tcaccatccc ctccatcaac
     2821 aacgagaccc ccggcatccg ctaccagtac aacgtgctgc cccagggctg gaagggctcc
     2881 cccgccatct tccagtgctc catgaccaag atcctggagc ccttccgcaa gcagaacccc
     2941 gacatcgtga tctaccagta catggacgac ctgtacgtgg gctccgacct ggagatcggc
     3001 cagcaccgca ccaagatcga ggagctgcgc cagcacctgc tgcgctgggg cttcaccacc
     3061 cccgacaaga agcaccagaa ggagcccccc ttcctgtgga tgggctacga gctgcacccc
     3121 gacaagtgga ccgtgcagcc catcgtgctg cccgagaagg actcctggac cgtgaacgac
     3181 atccagaagc tggtgggcaa gctgaactgg gcctcccaga tctacgccgg catcaaagtc
     3241 cgccagctgt gcaagctgct gcgcggcacc aaggccctga ccgaggtggt gcccctgacc
     3301 gaggaggccg agctggagct ggccgagaac cgcgagatcc tgaaggagcc cgtgcacggc
     3361 gtgtactacg acccctccaa ggacctgatc gccgagatcc agaagcaggg ccagggccag
     3421 tggacctacc agatctacca ggagcccttc aagaacctga agaccggcaa atacgcccgc
     3481 atgaagggcg cccacaccaa cgacgtgaag cagctgaccg aggccgtgca gaagatcgcc
     3541 accgagtcca tcgtgatctg gggcaagact cccaagttca agctgcccat ccagaaggag
     3601 acctgggagg cctggtggac cgagtactgg caggccacct ggatccccga gtgggagttc
     3661 gtgaacaccc cccccctggt gaagctgtgg taccagctgg agaaggagcc catcatcggc
     3721 gccgagacct tctacgtgga cggcgccgcc aaccgcgaga ccaagctggg caaggccggc
     3781 tacgtgaccg accgcggccg ccagaaggtg gtgcccctga ccgacaccac caaccagaag
     3841 accgagctgc aggccatcca cctggccctg caagactccg gcctggaggt gaacatcgtg
     3901 accgactccc agtatgcatt gggcatcatc caggcccagc ccgacaagtc cgagtccgag
     3961 ctggtgtccc agatcatcga gcagctgatc aagaaggaga aggtgtacct ggcctgggtg
     4021 cccgcccaca agggcatcgg cggcaacgag caggtggaca agctggtgtc cgccggcatc
     4081 cgcaaggtgc tgttcctgga cggcatcgac aaggcccagg aggagcacga gaagtaccac
     4141 tccaactggc gcgccatggc ctccgacttc aacctgcccc ccgtggtggc caaggagatc
     4201 gtggcctcct gcgacaagtg ccagctgaag ggcgaggcca tgcacggcca ggtggactgc
     4261 tcccccggca tctggcagct ggactgcacc cacctggagg gcaaggtgat cctggtggcc
     4321 gtgcacgtgg cctccggcta catcgaggcc gaggtgatcc ccgccgagac cggccaggag
     4381 accgcctact tcctgctgaa gctggccggc cgctggcccg tgaagaccgt gcacaccgac
     4441 aacggctcca acttcacctc caccaccgtg aaggccgcct gctggtgggc cggcatcaag
     4501 caggagttcg gcatccccta caacccccag tcccagggcg tgatcgagtc catgaacaag
     4561 gagctgaaga agatcatcgg ccaagtccgc gaccaggccg agcacctgaa gaccgccgtg
     4621 cagatggccg tgttcatcca caacttcaag cgcaagggcg gcatcggcgg ctactccgcc
     4681 ggcgagcgca tcgtggacat catcgccacc gacatccaga ccaaggagct gcagaagcag
     4741 atcaccaaga tccagaactt ccgcgtgtac taccgcgact cccgcgaccc cgtgtggaag
     4801 ggccccgcca agctgctgtg gaagggcgag ggcgccgtgg tgatccagga caactccgac
     4861 atcaaggtgg tgccccgccg caaggccaag atcatccgcg actacggcaa gcagatggcc
     4921 ggcgacgact gcgtggcctc ccgccaggac gaggactaac acatggaaaa gattagtaaa
     4981 acaccatagg ccgctctaga ggatccaagc ttatcgatac cgtcgacctc gagggcccag
     5041 atctaattca ccccaccagt gcaggctgcc tatcagaaag tggtggctgg tgtggctaat
     5101 gccctggccc acaagtatca ctaagctcgc tttcttgctg tccaatttct attaaaggtt
     5161 cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct
     5221 ggattctgcc taataaaaaa catttatttt cattgcaatg atgtatttaa attatttctg
     5281 aatattttac taaaaaggga atgtgggagg tcagtgcatt taaaacataa agaaatgaag
     5341 agctagttca aaccttggga aaatacacta tatcttaaac tccatgaaag aaggtgaggc
     5401 tgcaaacagc taatgcacat tggcaacagc ccctgatgcc tatgccttat tcatccctca
     5461 gaaaaggatt caagtagagg cttgatttgg aggttaaagt tttgctatgc tgtattttac
     5521 attacttatt gttttagctg tcctcatgaa tgtcttttca ctacccattt gcttatcctg
     5581 catctctcag ccttgactcc actcagttct cttgcttaga gataccacct ttcccctgaa
     5641 gtgttccttc catgttttac ggcgagatgg tttctcctcg cctggccact cagccttagt
     5701 tgtctctgtt gtcttataga ggtctacttg aagaaggaaa aacagggggc atggtttgac
     5761 tgtcctgtga gcccttcttc cctgcctccc ccactcacag tgacccggaa tccctcgaca
     5821 tggcagtcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     5881 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6061 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6121 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6181 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6241 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6301 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6361 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6421 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6481 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6541 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6601 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6661 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6721 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6781 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6841 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6901 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacggaga
     7681 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     7741 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     7801 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     7861 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     7921 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     7981 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     8041 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     8101 aatggggaag gccatccagc ctcgcgtcgg ggagcttttt gcaaaagcct aggcctccaa
     8161 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     8221 aaataaaaaa aattagtcag ccatgagctt ggcccattgc atacgttgta tccatatcat
     8281 aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg
     8341 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     8401 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     8461 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     8521 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     8581 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     8641 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     8701 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     8761 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     8821 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     8881 gtacggtggg aggtctatat aagcagagct
//
