LOCUS       sequence-323681-         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Venus fused to the N-terminus of the Bcl-2 family protein, 
            Bik with mutation in BH3 domain to disrupt binding anti-apoptotic 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, 
            Fang Q, Andrews DW
  TITLE     Efficacy and specificity of inhibitors of BCL-2 family protein 
            interactions assessed by affinity measurements in live cells.
  JOURNAL   Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. 
            Epub 2022 Apr 20.
  PUBMED    35442739
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-04-22"; volume: "8(16):eabm7375. doi"; pages: " 
            10.1126/sciadv.abm7375. Epub 2022 Apr 20"
COMMENT     Sequence Label: Venus-Bik-4E-pEGFP-C1
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctgaag taagacccct ctccagagac atcttgatgg agaccctcct gtatgagcag
       61 ctcctggaac ccccgaccat ggaggttctt ggcatgactg actctgaaga ggacctggac
      121 cctatggagg acttcgattc tttggaatgc atggagggca gtgacgcaga ggccctgcgg
      181 gaggcctgcg aaggggacga ggaggacgtg agcctcaggg ccccgcgcct ggcccagctc
      241 tccgaggtgg ccatgcacag cctgggtctg gctttcatct acgaccagac tgaggacatc
      301 agggatgttc ttagaagttt catggacggt ttcaccacac ttaaggagaa cataatgagg
      361 ttctggagat ccccgaaccc cgggtcctgg gtgtcctgcg aacaggtgct gctggcgctg
      421 ctgctgctgc tggcgctgct gctgccgctg ctcagcgggg gcctgcacct gctgctcaag
      481 tga
//
