LOCUS       sequence-323685-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Venus fused to the N-terminus of the Bcl-2 family protein, 
            Puma with mutation in BH3 domain to disrupt binding anti-apoptotic 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, 
            Fang Q, Andrews DW
  TITLE     Efficacy and specificity of inhibitors of BCL-2 family protein 
            interactions assessed by affinity measurements in live cells.
  JOURNAL   Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. 
            Epub 2022 Apr 20.
  PUBMED    35442739
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-04-22"; volume: "8(16):eabm7375. doi"; pages: " 
            10.1126/sciadv.abm7375. Epub 2022 Apr 20"
COMMENT     Sequence Label: Venus-Puma-4E-pEGFP-C1
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcacgcg cacgtcagga aggcagttca cctgaaccag tggaaggact ggcccgggat
       61 gggccgcgtc catttccgct tgggcgcctg gttccgtcag ccgtctcttg cggactgtgc
      121 gagccgggtc tggctgccgc accggctgca cccaccttac tgcccgccgc ttacctttgc
      181 gcgcctacgg ctcccccagc agtaacggcc gccctgggag ggagccgttg gccgggcggc
      241 cctcgtagcc gtccccgggg tccacgcccg gatggtccac agccaagtct gtcactggct
      301 gaacaacatc ttgaatctcc cgtgccatca gcaccaggag ctctggctgg tggcccgaca
      361 caagcagcac cgggggttcg gggcgaagaa gaacagtggg cccgggaaga aggtgcgcag
      421 gaacgtcgcg aggccgatga tgagaacgcc cagtatgagc gtcgccgcca ggaggagcag
      481 cagcgtcatc gcccctctcc ttggcgcgta ctttacaacc tgattatggg cttactgccc
      541 ttaccgcgtg gccaccgggc tcccgagatg gaacccaatt aa
//
