LOCUS       sequence-32369-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Kelker MS, Page R, Peti W
  TITLE     Crystal structures of protein phosphatase-1 bound to nodularin-R and
            tautomycin: a novel scaffold for structure-based drug design of 
            serine/threonine phosphatase inhibitors.
  JOURNAL   J Mol Biol. 2009 Jan 9. 385(1):11-21.
  PUBMED    18992256
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Jan 9. 385(1):11-21."
COMMENT     Sequence Label: PP1 7-300
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacttctttg ggctttgtag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc tttcatttct tggctttggc agagttgcgg ggtggggtga tgggtcggcc
      121 tccagggttc aggccactga actgcccgta cttgcccttg ttcttgtcgg ccggcttaag
      181 gatctggaag gagcacatga gggtctcgtc cacgctcatc atggctccgg cgttgtcgaa
      241 ctcgccacag tagttggggg ctgagaaaag tgtcaccagc tgccgcttgg caaagaactc
      301 atagccgtcc tccaccacct ggtgcgcccg gcagatgagg tccaggtcat gcttgtgcag
      361 gaacttggcc accacctccg cgccgaaggt gaaggagacg ccgcggtcgt tctcgcccca
      421 gccctgcacg tccttgtcgg ggtcagacca cagcaggtca cacagcaggc cctggtcggg
      481 cacgtccgtg ggccgcatga tgcgccggat ctgctccatg gactgcaggt cgggggagag
      541 gccgccatgg cagcagaata tcttctcgtc cacaatggcc gcgatgggca ggcagttgaa
      601 gcagtcggtg aacgtcttcc acagcttgat gttgtagcgt ctcttgcact cgtcgtagaa
      661 gccgtagatg cggttgatgc tggcgcactc gtggttcccg cgcagcagga agaagtt
//
