LOCUS       sequence-323715-        6546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   White EA, Walther J, Javanbakht H, Howley PM
  TITLE     Genus beta human papillomavirus E6 proteins vary in their effects on
            the transactivation of p53 target genes.
  JOURNAL   J Virol. 2014 Aug;88(15):8201-12. doi: 10.1128/JVI.01197-14. Epub 
            2014 May 21.
  PUBMED    24850740
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01197-14"; journalName: "J Virol"; date: "2014-08"; 
            volume: "88"; issue: "15"; pages: "8201-12"
COMMENT     Sequence Label: p7270 MSCV-N-HA-IRES-PURO Empty
FEATURES             Location/Qualifiers
     source          1..6546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..479
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     409..431
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     447..463
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             513..539
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    555..679
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(704..709,710..727,728..734)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    759..1326
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    776..1328
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(920..937)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1147..1166
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1340..1939
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1340..1359)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1836..1856
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2015..2529
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2691..2707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2715..2721,2722..2739,2740..2745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2760..2781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2898..2915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3069..3657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3149..3168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3828..4619,4620..4688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4451..4470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4689..4793)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4861..4879
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4917..4939)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5039..5058
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5252..5274
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5402..5421
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5621..6137
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6201..6542
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
ORIGIN
        1 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
       61 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
      121 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
      181 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
      241 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
      301 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
      361 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
      421 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
      481 gaattagatc tgccaccatg ctcgatggag gatacccata cgatgttcca gattacgctg
      541 ctcgaggtta tcaaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
      601 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
      661 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
      721 cggctcgtat aatgtgtgga ttttgagtta ggatcaattc cgcccccccc ccctaacgtt
      781 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
      841 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
      901 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
      961 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     1021 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     1081 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     1141 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     1201 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     1261 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     1321 tgataatatg gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     1381 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     1441 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     1501 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     1561 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     1621 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     1681 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     1741 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     1801 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     1861 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     1921 ccgcaagccc ggtgcctgat gcatcgatgt cgacctgcag ccaagcttat cgataaaata
     1981 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     2041 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
     2101 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
     2161 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
     2221 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     2281 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     2341 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     2401 tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     2461 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     2521 gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg taggagtcga
     2581 atattaagta atcctgactc aattagccac tgttttgaat ccacatactc caatactcct
     2641 gaaatagttc attatggaca gcgcagaaag agctggggag aattgtgaaa ttgttatccg
     2701 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2761 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2821 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2881 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2941 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4141 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4441 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4741 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4801 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4861 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4921 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4981 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5041 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5101 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     5161 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5221 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcg
     5281 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     5341 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     5401 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     5461 cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata tcagtggtcc
     5521 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     5581 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     5641 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     5701 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     5761 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     5821 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     5881 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     5941 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     6001 cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc
     6061 atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt gactgcccac
     6121 ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc cagggaccac
     6181 cgaccccccc gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg
     6241 tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct gtactagtta gctaactagc
     6301 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
     6361 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
     6421 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     6481 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
     6541 tgcagc
//
