LOCUS       sequence-323717-       11103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN071
FEATURES             Location/Qualifiers
     source          1..11103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..587
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4474..4494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4843..4876
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4922..6278
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4922..5265
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5266..6075
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5333..5352)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6081..6278
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6326..6359
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6427..6447)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6427..6445)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6466..6482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6466..6482)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6479..6501)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6490..6506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6514..6520,6521..6538,6539..6544))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6559..6580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6697..6714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6868..7456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6948..6967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7627..8418,8419..8487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8250..8269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8488..8592)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8629..9132
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9173..9191
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9229..9251)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9351..9370
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9391..9671
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9672..10346
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10449..10904)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10586..10607)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10798..10817
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11030..11052
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11044..11061
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11045..11061
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11071..11090
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11071..11089
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttggctgata atagcgtata aacaatgcat actttgtacg ttcaaaatac aatgcagtag
       61 atatatttat gcatattaca tataatacat atcacatagg aagcaacagg cgcgttggac
      121 ttttaatttt cgaggaccgc gaatccttac atcacaccca atcccccaca agtgatcccc
      181 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagacacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt
      481 aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt
      541 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa
      601 atgtctaagt tggaaaaatt caccaactgt tactctttgt ctaagacttt gagattcaag
      661 gccatcccag ttggtaagac ccaagaaaac atcgacaaca agagactatt agttgaagat
      721 gaaaagagag ctgaagacta caagggtgtc aagaaattgt tggacagata ctacttgtct
      781 tttatcaacg acgttttgca ttccatcaag ctaaagaact tgaataacta catctctttg
      841 ttcagaaaga agactagaac tgaaaaggaa aataaggaat tggaaaactt ggaaatcaac
      901 ttgagaaagg aaattgctaa ggctttcaag ggtaatgaag gttacaagtc tttattcaag
      961 aaagacatca ttgaaaccat tttgccagaa tttttggatg ataaggatga aattgctttg
     1021 gttaactctt tcaacggttt caccactgct ttcactggtt tcttcgacaa cagagaaaac
     1081 atgttctccg aggaagctaa atccacttct attgctttca gatgtatcaa cgaaaacttg
     1141 acccgttaca tctctaacat ggacattttt gaaaaggtcg acgccatctt tgacaagcac
     1201 gaagtccaag aaatcaagga aaagatctta aactccgact acgatgtcga agatttcttc
     1261 gaaggtgaat tcttcaactt tgttttaacc caagaaggta tcgatgtcta caacgccatt
     1321 atcggtggtt ttgtcactga atctggtgaa aagatcaagg gtttgaacga atacattaac
     1381 ttgtacaacc aaaagaccaa acaaaaattg ccaaagttca agccattgta caagcaagtt
     1441 ttgtctgaca gagaatcttt gtctttttac ggtgaagggt acacctctga cgaagaagtc
     1501 ttggaagtct tcagaaacac tttgaacaag aactctgaaa tcttctcctc catcaagaag
     1561 ttagaaaagt tgttcaagaa cttcgatgaa tactcttctg ctggtatctt cgttaagaac
     1621 ggtccagcca tctctaccat ttctaaggat atctttggtg aatggaacgt cattagagac
     1681 aaatggaacg ctgaatacga tgacatccat ttgaagaaaa aggctgttgt caccgaaaag
     1741 tacgaagacg acagaagaaa atccttcaag aagatcggtt ccttctcctt ggaacaatta
     1801 caagaatacg ccgatgccga tttgtccgtt gtcgaaaaat tgaaggaaat tattattcaa
     1861 aaggttgatg aaatttacaa agtttacggt tcctctgaaa agttattcga tgctgatttc
     1921 gtcttggaaa agtctttgaa gaagaacgac gctgttgtcg ctatcatgaa ggacttgttg
     1981 gactctgtca aatctttcga aaactatatc aaggccttct tcggtgaagg taaggaaact
     2041 aacagagatg aatccttcta cggtgacttt gtcttggctt acgatatttt gttgaaggtt
     2101 gaccacatct acgatgccat cagaaactac gttactcaaa agccatactc taaggacaaa
     2161 ttcaagttgt acttccaaaa cccacaattc atgggtggtt gggataagga caaggaaact
     2221 gactacagag ctaccatttt gagatacggt tccaagtact acttggccat catggacaag
     2281 aagtacgcca agtgtttgca aaagattgac aaggacgatg tcaacggtaa ctacgaaaag
     2341 attaactaca agttgttgcc aggtccaaac aagatgttgc caaaggtttt cttctccaaa
     2401 aagtggatgg cttactacaa cccatctgaa gacatccaaa agatctacaa gaacggtact
     2461 ttcaaaaagg gtgacatgtt caacttaaac gactgtcaca agttgatcga cttcttcaag
     2521 gactccatct ctagataccc aaaatggtcc aacgcttacg atttcaactt ctctgaaact
     2581 gaaaaataca aggatattgc tggtttctac cgtgaagtcg aggaacaagg ttataaggtt
     2641 tctttcgaat ccgcttctaa gaaagaagtt gacaaattag tcgaagaagg taagttgtac
     2701 atgttccaaa tctacaacaa agatttctcc gacaagtctc acggtactcc aaacttgcac
     2761 accatgtact tcaagttgct attcgatgaa aacaaccacg gtcaaatcag attgtctggt
     2821 ggtgctgaat tgttcatgag acgtgcttct ctaaagaagg aagaattagt cgtccaccca
     2881 gctaactctc caattgccaa caagaaccca gacaacccta agaagaccac cactttgtcc
     2941 tacgacgttt acaaggacaa gagattctcc gaagaccaat acgaattgca cattccaatt
     3001 gctatcaaca agtgtccaaa gaacatcttc aagatcaaca ctgaagtcag agttttgtta
     3061 aagcacgatg acaaccctta cgttattggt atcgaccgtg gtgaaagaaa tttgttgtac
     3121 attgttgttg ttgacggtaa gggtaacatc gttgaacaat actccttgaa cgaaatcatc
     3181 aacaacttca acggtattag aatcaagact gattaccact ctttgttgga taagaaggaa
     3241 aaggaacgtt ttgaagctcg tcaaaactgg acctctattg aaaacatcaa agaattgaag
     3301 gctggttaca tcagtcaagt tgtccacaag atctgtgaat tggtcgagaa gtacgatgcc
     3361 gttattgcct tggcagattt gaactctggt tttaagaact ctcgtgtcaa ggttgaaaag
     3421 caagtctacc aaaagttcga aaagatgtta atcgacaaat tgaactacat ggttgacaag
     3481 aaatccaacc catgtgctac cggtggtgct ttgaaaggtt accaaatcac caacaaattc
     3541 gaatctttca aatctatgtc cactcaaaac gggttcatct tctacattcc agcttggttg
     3601 acctccaaga tcgacccatc taccggtttc gttaacttgt tgaagaccaa gtacacttcc
     3661 attgctgatt ccaagaagtt catctcttct ttcgacagaa tcatgtacgt tccagaagaa
     3721 gacttgttcg aattcgcctt ggactataag aacttctcca gaaccgatgc tgactacatt
     3781 aagaaatgga aattgtactc ctacggtaac agaatcagaa ttttcagaaa cccaaagaaa
     3841 aacaacgttt tcgattggga agaagtttgt ttgacttctg cctacaagga attattcaac
     3901 aaatacggta tcaactacca acaaggtgat atcagagctt tgttgtgtga acaatctgac
     3961 aaggctttct actcttcctt catggctttg atgtccttga tgttgcaaat gagaaactcc
     4021 atcactggta gaactgatgt cgacttcctc atttctccag ttaagaattc tgacggtatt
     4081 ttctacgact ctagaaatta cgaagctcaa gaaaacgcta ttttgccaaa gaacgctgat
     4141 gctaacggtg cttacaatat tgctagaaag gttttgtggg ctatcggtca attcaagaag
     4201 gctgaagacg aaaagctaga caaggtcaag attgctattt ctaacaagga atggttggaa
     4261 tacgctcaaa cctccgtcaa gcacggtagt tccaagcttg gcggcagcgg cggcagcatg
     4321 gaacgtgtca gaatgatcaa cgttcaaaga ttgttggaag ctgctgaatt cttggaaaga
     4381 agagaaagag aatgtgaaca cggttacgcc tcctctttcc catccatgcc atctccaaga
     4441 ggtagttcca agcttggcgg cagcggcggc agcccaaaga agaagagaaa ggtataaagg
     4501 agttaaaggc aaagttttct tttctagagc cgttcccaca aataattata cgtatatgct
     4561 tcttttcgtt tactatatat ctatatttac aagcctttat tcactgatgc aatttgtttc
     4621 caaatacttt tttggagatc tcataactag atatcatgat ggcgcaactt ggcgctatct
     4681 taattactct ggctgccagg cccgtgtaga gggccgcaag accttctgta cgccatatag
     4741 tctctaagaa cttgaacaag tttctagacc tattgccgcc tttcggatcg ctattgttgc
     4801 ggccgccagc tgaagcttcg tacgctgcag gtcgacgaat tctaccgttc gtataatgta
     4861 tgctatacga agttatagat ctgtttagct tgcctcgtcc ccgccgggtc acccggccag
     4921 cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg
     4981 atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat
     5041 acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga
     5101 gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag
     5161 aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat
     5221 cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg taaggaaaag
     5281 actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     5341 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
     5401 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     5461 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     5521 atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa aacagcattc
     5581 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     5641 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     5701 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     5761 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
     5821 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     5881 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     5941 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     6001 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     6061 gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat
     6121 ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata
     6181 ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat
     6241 catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc
     6301 gccatccagt gtcgaaaacg agctcataac ttcgtataat gtatgctata cgaacggtag
     6361 aattcgatat cagatccact agtggcctat gcggccgcca ccgcggtgga gctccagctt
     6421 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     6481 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6541 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6601 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6661 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6721 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7201 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7321 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7441 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7501 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7561 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7621 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7681 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7741 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7801 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7861 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7921 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7981 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8041 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8101 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8161 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8221 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8281 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8341 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8401 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8461 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8521 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8581 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
     8641 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
     8701 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
     8761 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
     8821 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
     8881 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
     8941 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
     9001 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
     9061 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
     9121 aggcaagcga tccgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9181 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9241 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9301 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     9361 gcagattgta ctgagagtgc accatagatc acgacattac tatatatata atataggaag
     9421 catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc cagatggcag
     9481 tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat cttgatccgg
     9541 agcttttctt tttttgccga ttaagaatta attcggtcga aaaaagaaaa ggagagggcc
     9601 aagagggagg gcattggtga ctattgagca cgtgagtata cgtgattaag cacacaaagg
     9661 cagcttggag tatgtctgtt attaatttca caggtagttc tggtccattg gtgaaagttt
     9721 gcggcttgca gagcacagag gccgcagaat gtgctctaga ttccgatgct gacttgctgg
     9781 gtattatatg tgtgcccaat agaaagagaa caattgaccc ggttattgca aggaaaattt
     9841 caagtcttgt aaaagcatat aaaaatagtt caggcactcc gaaatacttg gttggcgtgt
     9901 ttcgtaatca acctaaggag gatgttttgg ctctggtcaa tgattacggc attgatatcg
     9961 tccaactgca tggagatgag tcgtggcaag aataccaaga gttcctcggt ttgccagtta
    10021 ttaaaagact cgtatttcca aaagactgca acatactact cagtgcagct tcacagaaac
    10081 ctcattcgtt tattcccttg tttgattcag aagcaggtgg gacaggtgaa cttttggatt
    10141 ggaactcgat ttctgactgg gttggaaggc aagagagccc cgaaagctta cattttatgt
    10201 tagctggtgg actgacgcca gaaaatgttg gtgatgcgct tagattaaat ggcgttattg
    10261 gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa agactctaac aaaatagcaa
    10321 atttcgtcaa aaatgctaag aaataggtta ttactgagta gtatttattt aagtattgtt
    10381 tgtgcacttg ccgatctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10441 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
    10501 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
    10561 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    10621 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    10681 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    10741 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    10801 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
    10861 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
    10921 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10981 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    11041 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
    11101 acc
//
