LOCUS       sequence-323728-       11136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN072
FEATURES             Location/Qualifiers
     source          1..11136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..587
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4297..4317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4507..4527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4876..4909
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4955..6311
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4955..5298
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5299..6108
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5366..5385)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6114..6311
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6359..6392
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6460..6480)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6460..6478)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6499..6515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6499..6515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6512..6534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6523..6539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6547..6553,6554..6571,6572..6577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6592..6613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6730..6747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6901..7489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6981..7000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7660..8451,8452..8520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8283..8302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8521..8625)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8662..9165
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9206..9224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9262..9284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9384..9403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9424..9704
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9705..10379
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10482..10937)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10619..10640)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10831..10850
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11063..11085
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11077..11094
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11078..11094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11104..11123
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11104..11122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttggctgata atagcgtata aacaatgcat actttgtacg ttcaaaatac aatgcagtag
       61 atatatttat gcatattaca tataatacat atcacatagg aagcaacagg cgcgttggac
      121 ttttaatttt cgaggaccgc gaatccttac atcacaccca atcccccaca agtgatcccc
      181 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagacacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt
      481 aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt
      541 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa
      601 atgtctaagt tggaaaaatt caccaactgt tactctttgt ctaagacttt gagattcaag
      661 gccatcccag ttggtaagac ccaagaaaac atcgacaaca agagactatt agttgaagat
      721 gaaaagagag ctgaagacta caagggtgtc aagaaattgt tggacagata ctacttgtct
      781 tttatcaacg acgttttgca ttccatcaag ctaaagaact tgaataacta catctctttg
      841 ttcagaaaga agactagaac tgaaaaggaa aataaggaat tggaaaactt ggaaatcaac
      901 ttgagaaagg aaattgctaa ggctttcaag ggtaatgaag gttacaagtc tttattcaag
      961 aaagacatca ttgaaaccat tttgccagaa tttttggatg ataaggatga aattgctttg
     1021 gttaactctt tcaacggttt caccactgct ttcactggtt tcttcgacaa cagagaaaac
     1081 atgttctccg aggaagctaa atccacttct attgctttca gatgtatcaa cgaaaacttg
     1141 acccgttaca tctctaacat ggacattttt gaaaaggtcg acgccatctt tgacaagcac
     1201 gaagtccaag aaatcaagga aaagatctta aactccgact acgatgtcga agatttcttc
     1261 gaaggtgaat tcttcaactt tgttttaacc caagaaggta tcgatgtcta caacgccatt
     1321 atcggtggtt ttgtcactga atctggtgaa aagatcaagg gtttgaacga atacattaac
     1381 ttgtacaacc aaaagaccaa acaaaaattg ccaaagttca agccattgta caagcaagtt
     1441 ttgtctgaca gagaatcttt gtctttttac ggtgaagggt acacctctga cgaagaagtc
     1501 ttggaagtct tcagaaacac tttgaacaag aactctgaaa tcttctcctc catcaagaag
     1561 ttagaaaagt tgttcaagaa cttcgatgaa tactcttctg ctggtatctt cgttaagaac
     1621 ggtccagcca tctctaccat ttctaaggat atctttggtg aatggaacgt cattagagac
     1681 aaatggaacg ctgaatacga tgacatccat ttgaagaaaa aggctgttgt caccgaaaag
     1741 tacgaagacg acagaagaaa atccttcaag aagatcggtt ccttctcctt ggaacaatta
     1801 caagaatacg ccgatgccga tttgtccgtt gtcgaaaaat tgaaggaaat tattattcaa
     1861 aaggttgatg aaatttacaa agtttacggt tcctctgaaa agttattcga tgctgatttc
     1921 gtcttggaaa agtctttgaa gaagaacgac gctgttgtcg ctatcatgaa ggacttgttg
     1981 gactctgtca aatctttcga aaactatatc aaggccttct tcggtgaagg taaggaaact
     2041 aacagagatg aatccttcta cggtgacttt gtcttggctt acgatatttt gttgaaggtt
     2101 gaccacatct acgatgccat cagaaactac gttactcaaa agccatactc taaggacaaa
     2161 ttcaagttgt acttccaaaa cccacaattc atgggtggtt gggataagga caaggaaact
     2221 gactacagag ctaccatttt gagatacggt tccaagtact acttggccat catggacaag
     2281 aagtacgcca agtgtttgca aaagattgac aaggacgatg tcaacggtaa ctacgaaaag
     2341 attaactaca agttgttgcc aggtccaaac aagatgttgc caaaggtttt cttctccaaa
     2401 aagtggatgg cttactacaa cccatctgaa gacatccaaa agatctacaa gaacggtact
     2461 ttcaaaaagg gtgacatgtt caacttaaac gactgtcaca agttgatcga cttcttcaag
     2521 gactccatct ctagataccc aaaatggtcc aacgcttacg atttcaactt ctctgaaact
     2581 gaaaaataca aggatattgc tggtttctac cgtgaagtcg aggaacaagg ttataaggtt
     2641 tctttcgaat ccgcttctaa gaaagaagtt gacaaattag tcgaagaagg taagttgtac
     2701 atgttccaaa tctacaacaa agatttctcc gacaagtctc acggtactcc aaacttgcac
     2761 accatgtact tcaagttgct attcgatgaa aacaaccacg gtcaaatcag attgtctggt
     2821 ggtgctgaat tgttcatgag acgtgcttct ctaaagaagg aagaattagt cgtccaccca
     2881 gctaactctc caattgccaa caagaaccca gacaacccta agaagaccac cactttgtcc
     2941 tacgacgttt acaaggacaa gagattctcc gaagaccaat acgaattgca cattccaatt
     3001 gctatcaaca agtgtccaaa gaacatcttc aagatcaaca ctgaagtcag agttttgtta
     3061 aagcacgatg acaaccctta cgttattggt atcgaccgtg gtgaaagaaa tttgttgtac
     3121 attgttgttg ttgacggtaa gggtaacatc gttgaacaat actccttgaa cgaaatcatc
     3181 aacaacttca acggtattag aatcaagact gattaccact ctttgttgga taagaaggaa
     3241 aaggaacgtt ttgaagctcg tcaaaactgg acctctattg aaaacatcaa agaattgaag
     3301 gctggttaca tcagtcaagt tgtccacaag atctgtgaat tggtcgagaa gtacgatgcc
     3361 gttattgcct tggcagattt gaactctggt tttaagaact ctcgtgtcaa ggttgaaaag
     3421 caagtctacc aaaagttcga aaagatgtta atcgacaaat tgaactacat ggttgacaag
     3481 aaatccaacc catgtgctac cggtggtgct ttgaaaggtt accaaatcac caacaaattc
     3541 gaatctttca aatctatgtc cactcaaaac gggttcatct tctacattcc agcttggttg
     3601 acctccaaga tcgacccatc taccggtttc gttaacttgt tgaagaccaa gtacacttcc
     3661 attgctgatt ccaagaagtt catctcttct ttcgacagaa tcatgtacgt tccagaagaa
     3721 gacttgttcg aattcgcctt ggactataag aacttctcca gaaccgatgc tgactacatt
     3781 aagaaatgga aattgtactc ctacggtaac agaatcagaa ttttcagaaa cccaaagaaa
     3841 aacaacgttt tcgattggga agaagtttgt ttgacttctg cctacaagga attattcaac
     3901 aaatacggta tcaactacca acaaggtgat atcagagctt tgttgtgtga acaatctgac
     3961 aaggctttct actcttcctt catggctttg atgtccttga tgttgcaaat gagaaactcc
     4021 atcactggta gaactgatgt cgacttcctc atttctccag ttaagaattc tgacggtatt
     4081 ttctacgact ctagaaatta cgaagctcaa gaaaacgcta ttttgccaaa gaacgctgat
     4141 gctaacggtg cttacaatat tgctagaaag gttttgtggg ctatcggtca attcaagaag
     4201 gctgaagacg aaaagctaga caaggtcaag attgctattt ctaacaagga atggttggaa
     4261 tacgctcaaa cctccgtcaa gcactccaga gctgatccaa agaagaagag aaaggtaggt
     4321 agttccaagc ttggcggcag cggcggcagc atggaacgtg tcagaatgat caacgttcaa
     4381 agattgttgg aagctgctga attcttggaa agaagagaaa gagaatgtga acacggttac
     4441 gcctcctctt tcccatccat gccatctcca agaggtagtt ccaagcttgg cggcagcggc
     4501 ggcagcccaa agaagaagag aaaggtataa aggagttaaa ggcaaagttt tcttttctag
     4561 agccgttccc acaaataatt atacgtatat gcttcttttc gtttactata tatctatatt
     4621 tacaagcctt tattcactga tgcaatttgt ttccaaatac ttttttggag atctcataac
     4681 tagatatcat gatggcgcaa cttggcgcta tcttaattac tctggctgcc aggcccgtgt
     4741 agagggccgc aagaccttct gtacgccata tagtctctaa gaacttgaac aagtttctag
     4801 acctattgcc gcctttcgga tcgctattgt tgcggccgcc agctgaagct tcgtacgctg
     4861 caggtcgacg aattctaccg ttcgtataat gtatgctata cgaagttata gatctgttta
     4921 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     4981 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     5041 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     5101 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     5161 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     5221 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     5281 atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat
     5341 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     5401 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     5461 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     5521 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     5581 ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca
     5641 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     5701 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     5761 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     5821 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     5881 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     5941 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     6001 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     6061 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca
     6121 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     6181 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     6241 cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg
     6301 tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctcat
     6361 aacttcgtat aatgtatgct atacgaacgg tagaattcga tatcagatcc actagtggcc
     6421 tatgcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     6481 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6661 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7321 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7621 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7681 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7741 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7801 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7861 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7921 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7981 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8041 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8101 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8161 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8221 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8281 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8341 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8401 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8461 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8521 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8581 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8641 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
     8701 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
     8761 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
     8821 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     8881 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     8941 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     9001 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     9061 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     9121 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat
     9181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     9241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     9301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     9361 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag
     9421 atcacgacat tactatatat ataatatagg aagcatttaa tagaacagca tcgtaatata
     9481 tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt tattgaaaaa
     9541 tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc cgattaagaa
     9601 ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
     9661 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
     9721 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
     9781 aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga
     9841 gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
     9901 gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt
     9961 tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat gagtcgtggc
    10021 aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact
    10081 gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt
    10141 cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa
    10201 ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg ccagaaaatg
    10261 ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga
    10321 caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg
    10381 ttattactga gtagtattta tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg
    10441 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
    10501 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
    10561 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
    10621 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
    10681 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
    10741 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
    10801 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
    10861 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
    10921 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10981 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    11041 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
    11101 gcgtaatacg actcactata gggcgaattg ggtacc
//
